Anti-anaemic effect of ethanol leaf extract of Cnidosculus aconitifolius on cyclophosphomide-induced anaemia in rats

Jamila Abdulhamid Atata; Taiwo Oluwaseun Ayoola; Abdullateef Abiodun Ajadi; Sani Adamu; Aishat O. Olatunji; Khalid Talha Biobaku

doi:10.1515/jcim-2020-0006

Article

Anti-anaemic effect of ethanol leaf extract of Cnidosculus aconitifolius on cyclophosphomide-induced anaemia in rats

Jamila Abdulhamid Atata , Taiwo Oluwaseun Ayoola , Abdullateef Abiodun Ajadi , Sani Adamu , Aishat O. Olatunji and Khalid Talha Biobaku

Published/Copyright: May 15, 2020

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From the journal Journal of Complementary and Integrative Medicine Volume 18 Issue 1

Abstract

Background

The folkloric claim that Cnidoscolus aconitifolius (Chaya) could ameliorate anaemia requires scientific revalidation in anaemic models.

Objective

The aim of this study is to investigate the anti-anaemic effect of Cnidoscolus aconitifolius on cyclophosphamide-induced anaemia in rats.

Methods

The leaves of Cnidoscolus aconitifolius were collected, dried and extracted with ethanol. Twenty five Wistar Albino rats weighing 120–180 kg were used. Anaemia was induced in groups 2, 3, 4 and 5 with a single injection of cyclophosphamide, while group 1 served as a negative control without anemia and was treated with 0.5 mL of normal saline, Group 2 rats were treated with 100 mg/kg body weight of Cnidoscolus aconitifolius. Group 3 rats were treated with 500 mg/kg body weight of Cnidoscolus aconitifolius. Group 4 served as the positive control rats and was treated with 0.5 mL of normal saline. Group 5 which served as the standard control rats and were treated with 5 mg/kg body weight of standard drug Chemiron. The treated lasted for two weeks during which blood samples were collected from each rat for haematological analysis.

Results

The result shows that hematological parameters were elevated in groups 2, 3 and 5 rats treated with 100 mg/kg and 500 mg/kg body weight of ethanol extract of Cnidoscolus aconitifolius and chemiron respectively.

Conclusions

Ethanol leaf extract of Cnidoscolus aconitifolius at a graded dose of 500 mg/kg body weight had higher ameliorative effect on the haematological parameters of cyclophosphamide-induced anemia in rats.

Keywords: anaemia; Cnidoscolus aconitifolius; cyclophosphamide; haematinic; rat

Corresponding author: Jamila Abdulhamid Atata, Department of Veterinary Pathology, University of Ilorin, Ilorin, Nigeria, Phone: +234 8061671252, E-mail: atata.aj@unilorin.edu.ng

Acknowledgments

The authors are grateful to the Mr. Moshood Bolaji of the Clinical Pathology Laboratory at the Faculty of Veterinary Medicine, University of Ilorin, Kwara state, Nigeria, for his technical support.

Research funding: None declared.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
Employment or leadership: None declared.
Honorarium: None declared.

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Received: 2019-03-13

Accepted: 2020-02-10

Published Online: 2020-05-15

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Articles in the same Issue

https://doi.org/10.1515/jcim-2020-0006

Keywords for this article

anaemia; Cnidoscolus aconitifolius; cyclophosphamide; haematinic; rat