Modulatory effect of some citrus (Citrus limon, Citrus reticulata, Citrus maxima) peels on monoamine oxidase, phosphodiesterase-5 and angiotensin-1 converting enzyme activities in rat heart homogenate
Abstract
Background
Citrus peels have been reported useful in folk medicine for the management of cardiovascular diseases, but there is dearth of information on the possible mechanisms for their therapeutic action. The aim of this study was to investigate the effect of methanolic extracts from some citrus [lime (Citrus limon), tangerine (Citrus reticulata), shaddock (Citrus maxima)] peels on some enzymes relevant to the management of cardiovascular diseases [monoamine oxidase (MAO), phosphodiesterase-5 (PDE-5) and angiotensin-1-converting enzyme (ACE)].
Methods
Effect of methanolic extracts of lime, tangerine and shaddock peels on MAO, PDE-5 and ACE were carried out using standard methods. In addition, the ability of the extracts to prevent oxidative damage in rat heart homogenates was also investigated. Finally, the total polyphenol content of extracts was determined.
Results
The results revealed that methanolic extracts of lime, tangerine and shaddock peels inhibited MAO, PDE-5, ACE and pro-oxidants induced lipid peroxidation in rat heart homogenate in a concentration-dependent manner.
Conclusions
Findings in this study revealed citrus peel methanolic extracts as natural inhibitor of enzymes (MAO, PDE-5 and ACE) implicated in cardiovascular diseases. Therefore, citrus peels could help in the management of cardiovascular diseases possibly through inhibition of these enzymes.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
References
[1] Ogihara T, Matsuzaki M, Matsuoka H, Shimamoto K, Shimada K, Rakugi H, et al. The combination therapy of hypertension to prevent cardiovascular events (COPE) trial: rationale and design. Hyper Res. 2005;28:331–8.10.1291/hypres.28.331Suche in Google Scholar
[2] Hammoud RA, Vaccari CS, Nagamia SH, Khan BV. Regulation of the renin-angiotensin system in coronary atherosclerosis: a review of the literature. Vasc Health Risk Manag. 2007;3:937–45.Suche in Google Scholar
[3] Balasuriya BWN, Rupasinghe HPV. Plant flavonoids as angiotensin converting enzyme inhibitors in regulation of hypertension. Funct Foods Health Dis. 2011;5:172–88.10.31989/ffhd.v1i5.132Suche in Google Scholar
[4] Balarini CM, Leal MA, Gomes IBS, Pereira TMC, Gava AL, Meyrelles SS, et al. Sildenafil restores endothelial function in the apolipoprotein E knockout mouse. J Transl Med. 2013;11:3.10.1186/1479-5876-11-3Suche in Google Scholar
[5] Lugnier C. Cyclic nucleotide phosphodiesterase (PDE) superfamily: a new target for the development of specific therapeutic agents. Pharmacol Ther. 2006;109:366–98.10.1016/j.pharmthera.2005.07.003Suche in Google Scholar
[6] Takimoto E, Kass DA. Role of oxidative stress in cardiac hypertrophy and remodeling. Hypertension. 2007;49:241–8.10.1161/01.HYP.0000254415.31362.a7Suche in Google Scholar
[7] Di Lisa F, Kaludercic N, Carpi A, Menabo R, Giorgio M. Mitochondrial pathways for ROS formation and myocardial injury: the relevance of p66 (Shc) and monoamine oxidase. Basic Res Cardiol. 2009;104:131–9.10.1007/s00395-009-0008-4Suche in Google Scholar
[8] Belitz HD, Grosch W. Fruits and fruit products. In: Hadziyev D, editor. Food chemistry. Berlin, Heidelberg: Springer Verlag, 1999:748–99.10.1007/978-3-662-07281-3_19Suche in Google Scholar
[9] Amic D, Davidovic-Amic D, Beslo D, Trinajstic N. Structure-related scavenging activity relationship of flavonoids. Croatia Chem Acta. 2003;76:55–61.Suche in Google Scholar
[10] Singleton VL, Orthofor R, Lamuela-Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteau reagent. Methods Enzymol. 1999;299:152–78.10.1016/S0076-6879(99)99017-1Suche in Google Scholar
[11] Meda A, Lamien CE, Romito M, Millogo J, Nacoulma OG. Determination of the total phenolic, flavonoid and proline contents in Burkina Faso honey, as well as their radical scavenging activity. Food Chem. 2005;91:571–7.10.1016/j.foodchem.2004.10.006Suche in Google Scholar
[12] Cushman DW, Cheung HS. Spectrophotometric assay and properties of the angiotensin-1-converting enzyme of rabbit lung. Biochem Pharmacol. 1971;20:1637–48.10.1016/0006-2952(71)90292-9Suche in Google Scholar
[13] Oboh G, Adebayo AA, Ademosun AO, Boligon AA. In-vitro inhibition of phosphodiesterase-5 and arginase activities in rat penile tissue by two Nigerian herbs (Hunteria umbellata and Anogeissus leiocarpus). J Basic Clin Physiol Pharmacol. 2017;28:393–401.10.1515/jbcpp-2016-0143Suche in Google Scholar
[14] Nwanna EE, Adebayo AA, Oboh G, Ogunsuyi OB, Ademosun AO. Modulatory effects of alkaloid extract from Gongronema latifolium (utazi) and Lasianthera Africana (editan) on activities of enzymes relevant to neurodegeneration. J Dietary Supplements. 2018. DOI: 10.1080/19390211.2018.1426075Suche in Google Scholar
[15] Turski W, Turska E, Grossbel M. Modification of spectrophotometric method of determination of monoamine-oxidase. Enzyme. 1973;14:211–20.10.1159/000459482Suche in Google Scholar
[16] Shodehinde SA, Oyeleye SI, Olasehinde TA, Adebayo AA, Oboh G, Boligon AA. Lasianthera africana leaves inhibit α-amylase, α-glucosidase, angiotensin-1-converting enzyme activities and Fe2+-induced oxidative damage in pancreas and kidney homogenates. Orient Pharm Exp Med. 2017;17:41–9.10.1007/s13596-017-0256-zSuche in Google Scholar
[17] Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Annals Biochem. 1979;95:351–8.10.1016/0003-2697(79)90738-3Suche in Google Scholar
[18] Zhang ZL, Lia QL, Lia BG, Zhangb Y, Gaob XP, Lia CQ. Three angiotensin converting enzyme inhibitors from Rabdosia coetsa. Phytomed. 2008;15:386–8.10.1016/j.phymed.2007.09.013Suche in Google Scholar
[19] Umamaheswari M, Ajith MP, Asokkumar K. In vitro angiotensin converting enzyme inhibitory and antioxidant activities of seed extract of Apium graveolens Linn. Annals Biol Res. 2012;3:1274–82.Suche in Google Scholar
[20] Coates D. The angiotensin converting enzyme (ACE). Int J Biochem Cell Biol. 2003;35:769–73.10.1016/S1357-2725(02)00309-6Suche in Google Scholar
[21] Je JY, Park PJ, Kim EK, Ahn CB. Antioxidant and angiotensin I converting enzyme inhibitory activity of Bambusae caulis in Liquamen. Food Chem. 2009;13:932–5.10.1016/j.foodchem.2008.08.022Suche in Google Scholar
[22] Campos MRS, Guerrero LAC, Ancona DAB. Angiotensin-I converting enzyme inhibitory and antioxidant activities of peptide fractions extracted by ultrafiltration of cowpea Vigna unguiculata hydrolysates. J Sci Food Agric. 2010;90:2512–8.10.1002/jsfa.4114Suche in Google Scholar PubMed
[23] Oboh G, Ademosun AO. Shaddock peels (Citrus maxima) phenolic extracts inhibit α-amylase, α-glucosidase and angiotensin-i-converting enzyme activities: a nutraceutical approach to diabetes management. Diab Met Synd Clin Res Rev. 2011;5:148–52.10.1016/j.dsx.2012.02.008Suche in Google Scholar PubMed
[24] Pinto MD, Ranilla LG, Apostolidis E, Lajolo FM, Genovese MI, Shetty K. Evaluation of antihyperglycemia and antihypertension potential of native peruvian fruits using in vitro models. J Med Food. 2009;12:278–91.10.1089/jmf.2008.0113Suche in Google Scholar PubMed
[25] Ademiluyi AO, Oboh G. In vitro anti-diabetes and antihypertension potential of phenolic extracts of selected underutilized tropical legumes. J Basic Clin Physiol Pharmacol. 2012;23:17–25.10.1515/jbcpp-2011-0005Suche in Google Scholar
[26] Murphy MP. How mitochondria produce reactive oxygen species. Biochem J. 2009;417:1–13.10.1042/BJ20081386Suche in Google Scholar PubMed PubMed Central
[27] Turrens JF. Mitochondrial formation of reactive oxygen species. J Physiol. 2003;552:335–44.10.1113/jphysiol.2003.049478Suche in Google Scholar PubMed PubMed Central
[28] Villeneuve C, Guilbeau-Frugier C, Sicard P, Lairez O, Ordener C, Duparc T, et al. p53-PGC-1alpha pathway mediates oxidative mitochondrial damage and cardiomyocyte necrosis induced by monoamine oxidase-A upregulation: role in chronic left ventricular dysfunction in mice. Antioxid Redox Signal. 2013;18:5–18.10.1089/ars.2011.4373Suche in Google Scholar PubMed PubMed Central
[29] Youdim MB, Edmondson D, Tipton KF. The therapeutic potential of monoamine oxidase inhibitors. Nat Rev Neurosci. 2006;7:295–309.10.1038/nrn1883Suche in Google Scholar PubMed
[30] Lue TF. Erectile dysfunction. N Engl J Med. 2000;342:1802–13.10.1056/NEJM200006153422407Suche in Google Scholar PubMed
[31] Oboh G, Adebayo AA, Ademosun AO. Phenolic-rich extracts of Eurycoma longifolia and Cylicodiscus gabunensis inhibit enzymes responsible for the development of erectile dysfunction and are antioxidants. J Basic Clin Physiol Pharmacol. 2018. DOI: 10.1515/jbcpp-2017-0160Suche in Google Scholar PubMed
[32] Oboh G, Adebayo AA, Ademosun AO. Erection-stimulating, anti-diabetic and antioxidant properties of Hunteria umbellata and Cylicodiscus gabunensis water extractable phytochemicals. J Complement Integr Med. 2017. DOI: 10.1515/jcim-2016-0164Suche in Google Scholar PubMed
[33] Abdelwahab SI, Mohamed AH, Mohamed OY, Oall M, Taha MME, Mohan S, et al. Erectogenic effects of Clerodendron capitatum: involvement of phosphodiesterase type-5 inhibition. Evidence- Based Compl Alt Med. 2012;Article ID 137386: 6.10.1155/2012/137386Suche in Google Scholar PubMed PubMed Central
[34] Chan EC, Pannangpetch P, Woodman OL. Relaxation to flavones and flavonols in rat isolated thoracic aorta: mechanism of action and structure-activity relationships. J Cardiovasc Pharmacol. 2000;35:326–33.10.1097/00005344-200002000-00023Suche in Google Scholar PubMed
[35] Ko WC, Shih CM, Lai YH, Chen JH, Huang HL. Inhibitory effects of flavonoids on phosphodiesterase isozymes from guinea pig and their structure-activity relationships. Biochem Pharmacol. 2004;68:2087–94.10.1016/j.bcp.2004.06.030Suche in Google Scholar PubMed
[36] Ademosun AO, Oboh G, Passamonti S, Tramer F, Ziberna L, Boligon AA, et al. Inhibition of metalloproteinase and proteasome activities in colon cancer cells by citrus peel extracts. J Basic Clin Physiol Pharmacol. 2015;26:471–7.10.1515/jbcpp-2013-0127Suche in Google Scholar PubMed
[37] Dell’ Agli M, Bush PA, Dorey FJ. Polyphenols in orange peels (citrus species) enhances relaxation corpus cavernosum tissue: implications to erectile physiology and dysfunction. Can J Pharmacol. 2006;73:1714–26.Suche in Google Scholar
[38] Moon KH. Increased nitroxidative stress promotes mitochondrial dysfunction in alcoholic and nonalcoholic fatty liver diseases. Oxidative Med Cell Longevity. 2013;2013.10.1155/2013/781050Suche in Google Scholar PubMed PubMed Central
[39] Kaisserlian CE, Razzouq N, Astier A, Paul M. Sodium nitroprusside stability at 1 μg/mL in aqueous solutions. Eur J Hosp PharmSci. 2005;11:88–90.Suche in Google Scholar
[40] Calcerrada P, Peluffo G, Radi R. Nitric oxide-derived oxidants with a focus on peroxynitrite: molecular targets, cellular responses and therapeutic implications. Curr Pharm Des. 2011;17:3905–32.10.2174/138161211798357719Suche in Google Scholar PubMed
[41] Wagner C, Fachinetto R, Dalla Corte CL, Brito VB, Severo D, Dias GDOC, et al. Quercitrin, a glycoside form of quercetin, prevents lipid peroxidation in vitro. Brain Res. 2006;1107:192–8.10.1016/j.brainres.2006.05.084Suche in Google Scholar PubMed
[42] Chu Y, Sun J, Wu X, Liu RH. Antioxidant and antiproliferative activity of common vegetables. J Agric Food Chem. 2002;50:6910–6.10.1021/jf020665fSuche in Google Scholar PubMed
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Artikel in diesem Heft
- Pre-clinical Studies
- Terminalia Sericea aqueous leaf extract protects growing wistar rats against fructose-induced fatty liver disease
- Bioactive potential of Indian stinging plants leaf extract against pathogenic fungi
- In vitro anticholinesterase, antimonoamine oxidase and antioxidant properties of alkaloid extracts from kola nuts (Cola acuminata and Cola nitida)
- Neuroprotective Effects of the Anthocleista Schweinfurthii Gilg. (Loganiaceae) Stem Bark Extract in Postmenopause-Like Model of Ovariectomized Wistar Rats
- Antioxidant activity of carob seeds and chemical composition of their bean gum by– products
- Modulatory effect of some citrus (Citrus limon, Citrus reticulata, Citrus maxima) peels on monoamine oxidase, phosphodiesterase-5 and angiotensin-1 converting enzyme activities in rat heart homogenate
- Clinical Studies
- Ameliorative potential of acupressure on gestational diabetes mellitus: A randomized controlled trial
- Complementary medicine for laboring women: a qualitative study of the effects of reflexology
- Decreased anxiety after Dawood fasting in the pre-elderly and elderly
- Inhibitory effects of Tiliacora triandra (Colebr.) Diels on cholesterol absorption
- Evaluation of antipyretic activity of Belladonna and Pyrogenium ultrahigh dilutions in induced fever model
- Short Communication
- Effect of integrated yoga on anti-psychotic induced side effects and cognitive functions in patients suffering from schizophrenia
- Effect of mud pack to eyes on psychological variables in healthy volunteers: a pilot randomized controlled trial
- Review
- Ethnomedicinal, phytochemical and pharmacological profile of a mangrove plant Ceriops Decandra GriffDin Hou.
- Biological markers for the effects of yoga as a complementary and alternative medicine