Inhibitory effects of Tiliacora triandra (Colebr.) Diels on cholesterol absorption

Acharaporn Duangjai; Surasak Saokaew

doi:10.1515/jcim-2017-0169

Article

Inhibitory effects of Tiliacora triandra (Colebr.) Diels on cholesterol absorption

Acharaporn Duangjai and Surasak Saokaew

Published/Copyright: October 12, 2018

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From the journal Journal of Complementary and Integrative Medicine Volume 16 Issue 1

Abstract

Background

Natural supplements and herbal medicines have been attracted to use for managing elevated cholesterol levels. Tiliacora triandra (Colebr.) Diels (TT) or Yanang (in Thai) is commonly used as an ingredient in various types of Thai foods. In this study, we investigated the effect of methanolic TT leaf extract on cholesterol absorption by measuring the uptake and the efflux of cholesterol and cholesterol micellar solubility. In addition, we tested the effect of TT leaf extract on pancreatic lipase activity.

Methods

The uptake and efflux of cholesterol was determined by quantification of radioactivity in differentiated Caco-2 cells after treatment with radioactive cholesterol. Cholesterol mixed micelles were prepared for cholesterol uptake, efflux and solubility studies. The pancreatic lipase activity was determined using 4-methylumbelliferyl oleate as a substrate.

Results

Our finding showed that TT extract decreased the uptake of cholesterol by approximately 48% but did not affect the efflux of cholesterol. TT inhibited pancreatic lipase activity with the IC₅₀ at 273.5 μg/mL and also decreased cholesterol micellar solubility.

Conclusions

These findings suggest that TT leaf extract seems to be a potential candidate as cholesterol-lowering agents.

Keywords: Caco-2 cells; cholesterol uptake; micelles solubility; pancreatic lipase, T. Triandra; Yanang

Acknowledgments

We gratefully acknowledge the School of Medical Sciences, University of Phayao and the Faculty of Pharmacy, Naresuan University for providing place to do the research. We also thank to Mr. Winai Saokaew and Mrs. Prayoon Saokaew for providing Tiliacora triandra leaves.

Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis and interpretation of data; in the writing of the report or in the decision to submit the report for publication.

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Received: 2017-12-07

Accepted: 2018-08-20

Published Online: 2018-10-12

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Articles in the same Issue

https://doi.org/10.1515/jcim-2017-0169

Keywords for this article

Caco-2 cells; cholesterol uptake; micelles solubility; pancreatic lipase, T. Triandra; Yanang