Gentiana kurroo Royle attenuates the metabolic aberrations in diabetic rats; Swertiamarin, swertisin and lupeol being the possible bioactive principles
-
Khalid Ghazanfar
,
Seema Akbar
und
Akbar Masood
Abstract
Background
Gentiana kuroo Royle is a medicinally important plant of north-western Himalayas used for various ailments. In the present study, the plant extracts were investigated for the antidiabetic effects in streptozotocin-induced diabetic rats.
Methods
The impact of the extracts on serum glucose levels of diabetic rats was compared with reference drug – glibenclamide-treated diabetic rats. Streptozotocin injection was used to induce diabetes in fasted rats. Various biochemical, physiological and histopathological parameters in diabetic rats were observed for assessing the antidiabetic activity.
Results
The serum glucose concentrations in diabetic rats were significantly lowered by the extracts (methanolic and hydroethanolic at the doses of 250 and 500 mg/kg body weight). Several related biochemical parameters like creatinine, low-density lipoproteins, triglycerides, cholesterol, alkaline phosphatase, serum glutamate oxaloacetate transaminase and serum glutamate pyruvate transaminase were likewise decreased by the concentrates. The extracts also showed reduction in feed and water consumption of diabetic rats when compared with the diabetic control. The extracts were found to demonstrate regenerative/protective effect on β-cells of pancreas in diabetic rats. The methanolic and hydroethanolic extracts also exhibited hypoglycaemic effect in normal glucose-fed rats (oral glucose tolerance tests). LC-MS characterization of this extract showed the presence of these compounds – Swertiamarin, swertisin, lupeol, etc.
Conclusions
The current study demonstrated the counter diabetic capability of G. kuroo Royle being powerful in hyperglycaemia and can viably ensure against other metabolic deviations created by diabetes in rats. The possible bioactive principles responsible for the antidiabetic activity of G. kurroo Royle are Swertiamarin, swertisin and lupeol.
Acknowledgments
Authors are thankful to the Director General, Central Council for Research in Unani Medicine, New Delhi, India for financial assistance in the form of JRF and providing necessary infrastructure to carry out the work. The authors are also thankful to DST, GoI, New Delhi for funding the project entitled “Establishment of National Facility for Non Clinical Safety Evaluation of Unani Drugs". We also pay gratitude to Mr Showkat Ahmad Teli, Mr. Bashir Ahmad Bhat, Mr Ashiq Ahmad Bhat and Mr. Shafiq Ahmad Khan for their valuable contribution in sample collection, animal handling and the maintenance of animal house.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
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Artikel in diesem Heft
- Review
- Cornus mas: a review on traditional uses and pharmacological properties
- Preclinical Studies
- Anti-inflammatory and immunomodulatory activities of Inula cappa roots (Compositae)
- Effects of water extract of Curcuma longa (L.) roots on immunity and telomerase function
- Garcinia kola seeds may prevent cognitive and motor dysfunctions in a type 1 diabetes mellitus rat model partly by mitigating neuroinflammation
- Changes in some biochemical parameters of alloxanized rats administered with varying concentrations of quail egg solution
- Ameliorative potential of Blighia sapida K.D. Koenig bark against pancreatic β-cell dysfunction in alloxan-induced diabetic rats
- Comparative evaluation of hypoglycemic and hypolipidemic activity of various extract of Anogeissus latifolia bark in streptozotocin-induced diabetic rats
- Gentiana kurroo Royle attenuates the metabolic aberrations in diabetic rats; Swertiamarin, swertisin and lupeol being the possible bioactive principles
- Biochemical and electrocardiographic studies on the beneficial effects of gallic acid in cyclophosphamide-induced cardiorenal dysfunction
- Clinical Studies
- Development and validation of a yoga module for Parkinson disease
- Case Report
- Return to work program efficacy with Self-Regulation Therapy (SRT®): Case study with complex trauma and concurrent disorders
