Histopathological and biochemical assessments of Costus afer stem on alloxan-induced diabetic rats
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Anthoneth Ndidi Ezejiofor
Abstract
Background:
In continuation of our study of the biological activity of the aqueous extract of Costus afer stem used in tradomedicare in Nigeria, this work has investigated the biochemical, nephroprotective, hepatoprotective and protective effects of aqueous extract of C. afer stem on alloxan-induced hyperglycaemic albino Wistar rats.
Methods:
Thirty male albino Wistar rats (150−200 g) were weight-matched into six groups of five rats each. Groups 1 and 2 were the normal (non-induced) and toxic (alloxan-induced) controls, respectively. Groups 3−6 were induced and treated with 1, 2 and 3 g/kg of the aqueous extract of C. afer stem and glibenclamide (5 mg/kg), respectively. Food and fluid intake, body weight, absolute and relative weight of organs (liver, pancreas, and kidneys) and biochemical parameters were measured.
Results:
There was a significant reduction in alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total bilirubin triglyceride, creatinine, urea and electrolyte when compared with toxic control group. The liver marker enzymes were restored in all the treated groups. The aqueous stem extract possessed hypoglycaemic effect and reversed the histopathological damage in alloxan-induced diabetic rats.
Conclusions:
This study suggests that aqueous stem extract of C. afer possesses both hypoglycaemic and organ protective properties.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
References
1. Jimoh A, Tanko Y, Mohammed A. Anti-diabetic effect of methanolic leaf of cissus cornifolia on alloxan induced hyperglycaemic wistar rats. Ann Biol Res 2013;4:46–54.Suche in Google Scholar
2. Babitha S, Banji D, Banji OJ. Antioxidant and hepatoprotective effects of flower extract of Millingtonia hortensis Linn. on carbon tetrachloride induced hepatotoxicity. J Pharm Bioallied Sci 2012;4:307–12.10.4103/0975-7406.103258Suche in Google Scholar
3. Kidd PM. Glutathione (Monograph). In: Czap K, Miller AL, Head KA, et al., editors. Alternative medicine review monographs volume one. Dover, ID: Thorne Research, Inc., 2002: 184–92.Suche in Google Scholar
4. Iwu MM. Handbook of African medicinal plants [M]. Boca Raton, FL, USA: CRC Press, 1993:464.Suche in Google Scholar
5. Oliver-Bever BE. Medicinal plants in tropical West Africa. Cambridge, UK: Cambridge University Press, 1986.10.1017/CBO9780511753114Suche in Google Scholar
6. Ezejiofor AN, Orish CN, Orisakwe OE. Effect of aqueous leaves extract of Costus afer Ker Gawl (Zingiberaceae) on the liver and kidney of male albino Wistar rat. Ancient Sci Life 2013;33:4–9.10.4103/0257-7941.134554Suche in Google Scholar
7. Nandhakumar J, Sethumathi PP, Malini A, Sengottuvelu S, Duralsamy R, Karthikeyan D, et al. Antidiabetic activity of methanol leaf extract of costus pictus D. Don in alloxan induced diabetic rats. J Health Sci 2007;53:655–63.10.1248/jhs.53.655Suche in Google Scholar
8. Jayasri MA, Gunasekaran S, Radha A, Mathew TL. Antidiabetic effect of Costus pictus leaves in normal and streptozotocin-induced diabetic rats. J Diabetes Metab 2008;16:117–22.10.1159/000497662Suche in Google Scholar
9. Udem SC, Ezeasor CK. The acute and subchronic toxicity studies of aqueous leaf and stem bark extract of Costus afer ker (zingiberaceae) in mice. Comp Clin Pathol 2010;19: 75–80.10.1007/s00580-009-0906-8Suche in Google Scholar
10. Reyes BA, Bautista ND, Tanquilut NC, Anunciado RV, Leung AB, Sanchez GC, et al. Anti-diabetic potentials of Momordica charantia and Andrographis paniculata and their effects on estrous cyclicity of alloxan-induced diabetic rats. J Ethnopharmacol 2006;105:196–200.10.1016/j.jep.2005.10.018Suche in Google Scholar
11. Mahmoudzadeh-Sagheb H, Heidari Z, Shahraki M, Moudi B. A stereological study of effects of aqueous extract of Tamarindus indica seeds on pancreatic islets in streptozotocin-induced diabetic rats. Pak J Pharm Sci 2010;23:427–34.Suche in Google Scholar
12. Abdel-Barry JA, Abdel-Hassan IA, Al-Hakeim MHH. Hypoglycaemic and antihyperglycaemic effects of Trigonella foenum-graecum leaf in normal and alloxan induced diabetic rats. J Ethnopharmacol 1997;58:149–55.10.1016/S0378-8741(97)00101-3Suche in Google Scholar
13. Nannipieri M, Gonzales C, Baldi S, Posadas R, Williams K, Haffner SM, et al. Liver enzymes, metabolic syndrome, and incident diabetes: the Mexico City diabetes study. Diabetes Care 2005;28:1757–62.10.2337/diacare.28.7.1757Suche in Google Scholar PubMed
14. Villegas R, Xiang YB, Elasy T, Cai Q, Xu W, Li H, et al. Liver enzymes, type 2 diabetes, and metabolic syndrome in middle-aged, urban Chinese men. Metab Syndr Relat Disord 2011;9:305–11.10.1089/met.2011.0016Suche in Google Scholar PubMed PubMed Central
15. Najmi AK, Pillai KK, Pal SN, Aqil M. Free radical scavenging and hepatoprotective activity of jigrine against galactosamine induced hepatotoxicity in rats. J Ethnopharmacol 2005;97: 521–5.10.1016/j.jep.2004.12.016Suche in Google Scholar PubMed
16. Rajesh V, Perumal P, Sundarrajan T. Antidiabetic activity of methanol extract of Smilax zeylanica Linn in streptozotocin induced diabetic rats. Intern J Pharmacol 2010;6. Available at: https://ispub.com/IJEN/6/1/10507.10.5580/1d39Suche in Google Scholar
17. Felig P, Mark E, Ohman JL, Lahil CF. Plasma amino acids level in diabetic ketoacidosis. Diabetes 1970;19:727–8.10.2337/diab.19.10.727Suche in Google Scholar
18. Larson RA. The antioxidants of higher plants. Phytochemistry 1988;27:969–78.10.1016/0031-9422(88)80254-1Suche in Google Scholar
©2017 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- Editorial
- The double face of light effects: circadian adjustment or disruption
- Review
- Artificial light-at-night – a novel lifestyle risk factor for metabolic disorder and cancer morbidity
- Behavior and Neuroprotection
- Anti-stress effects of a GSK-3β inhibitor, AR-A014418, in immobilization stress of variable duration in mice
- Cardiovascular Function
- Addition of omega-3 fatty acid and coenzyme Q10 to statin therapy in patients with combined dyslipidemia
- Oxidative Stress
- Protective effect of Moringa oleifera oil against HgCl2-induced hepato- and nephro-toxicity in rats
- Effects of peripherally and centrally applied ghrelin on the oxidative stress induced by renin angiotensin system in a rat model of renovascular hypertension
- Investigation of the role of α-lipoic acid on fatty acids profile, some minerals (zinc, copper, iron) and antioxidant activity against aluminum-induced oxidative stress in the liver of male rats
- Metabolism
- The choice of freely preferred cadence by trained nonprofessional cyclists may not be characterized by mechanical efficiency
- Immune Response
- Ascorbic acid does not modulate potassium currents in cultured human lymphocytes
- Phytotherapy
- Anti-inflammatory activity of Elaeagnus angustifolia fruit extract on rat paw edema
- Histopathological and biochemical assessments of Costus afer stem on alloxan-induced diabetic rats
- In vitro inhibition of phosphodiesterase-5 and arginase activities from rat penile tissue by two Nigerian herbs (Hunteria umbellata and Anogeissus leiocarpus)
- Antioxidant and antiproliferative potentials of methanol extract of Xylopia aethiopica (Dunal) A. Rich in PC-3 and LNCaP cells
Artikel in diesem Heft
- Frontmatter
- Editorial
- The double face of light effects: circadian adjustment or disruption
- Review
- Artificial light-at-night – a novel lifestyle risk factor for metabolic disorder and cancer morbidity
- Behavior and Neuroprotection
- Anti-stress effects of a GSK-3β inhibitor, AR-A014418, in immobilization stress of variable duration in mice
- Cardiovascular Function
- Addition of omega-3 fatty acid and coenzyme Q10 to statin therapy in patients with combined dyslipidemia
- Oxidative Stress
- Protective effect of Moringa oleifera oil against HgCl2-induced hepato- and nephro-toxicity in rats
- Effects of peripherally and centrally applied ghrelin on the oxidative stress induced by renin angiotensin system in a rat model of renovascular hypertension
- Investigation of the role of α-lipoic acid on fatty acids profile, some minerals (zinc, copper, iron) and antioxidant activity against aluminum-induced oxidative stress in the liver of male rats
- Metabolism
- The choice of freely preferred cadence by trained nonprofessional cyclists may not be characterized by mechanical efficiency
- Immune Response
- Ascorbic acid does not modulate potassium currents in cultured human lymphocytes
- Phytotherapy
- Anti-inflammatory activity of Elaeagnus angustifolia fruit extract on rat paw edema
- Histopathological and biochemical assessments of Costus afer stem on alloxan-induced diabetic rats
- In vitro inhibition of phosphodiesterase-5 and arginase activities from rat penile tissue by two Nigerian herbs (Hunteria umbellata and Anogeissus leiocarpus)
- Antioxidant and antiproliferative potentials of methanol extract of Xylopia aethiopica (Dunal) A. Rich in PC-3 and LNCaP cells
