Ascorbic acid does not modulate potassium currents in cultured human lymphocytes
-
Saravanakumar Snekalatha
and
Praghalathan Kanthakumar
Abstract
Background:
Ascorbic acid (AA) is known to modulate lymphocyte function, but the mechanism of action is not clearly understood. As voltage-gated potassium currents play an important role in lymphocyte function, the effect of AA on voltage-gated potassium currents was studied.
Methods:
Peripheral blood mononuclear cells were cultured in the presence of increasing concentrations of AA (0, 0.125, 0.25, 0.5, and 1 mM). Potassium currents in resting lymphocytes were studied by whole cell patch clamp technique using a depolarizing protocol. Lymphocyte function was assessed by measuring interleukin-2 (IL-2) secretion after mitogenic stimulation by ELISA.
Results:
The mean current density of potassium currents recorded from cells cultured for 48 h in the presence of 0.125 mM AA was not significantly different from that of cells cultured in the absence of AA. There was about 50% inhibition of IL-2 secretion in cell cultures with 0.125 mM AA when compared to controls without AA. At higher concentrations of AA, the IL-2 secretion decreased further.
Conclusions:
The results of the study indicate that the inhibition of lymphocyte function by AA in vitro may not be due to inhibition of potassium currents in the concentration tested.
Acknowledgments
The authors are thankful to Christian Medical College Fluid Research Grant for funding this project. Thanks to Dr. Abitha Sukumaran and Mr. Soosai Manickam for their help and support.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s)played no role in thestudy design; in thecollection, analysis, and interpretationof data; in the writing of the report; or in the decision tosubmit the report for publication.
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©2017 Walter de Gruyter GmbH, Berlin/Boston
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Articles in the same Issue
- Frontmatter
- Editorial
- The double face of light effects: circadian adjustment or disruption
- Review
- Artificial light-at-night – a novel lifestyle risk factor for metabolic disorder and cancer morbidity
- Behavior and Neuroprotection
- Anti-stress effects of a GSK-3β inhibitor, AR-A014418, in immobilization stress of variable duration in mice
- Cardiovascular Function
- Addition of omega-3 fatty acid and coenzyme Q10 to statin therapy in patients with combined dyslipidemia
- Oxidative Stress
- Protective effect of Moringa oleifera oil against HgCl2-induced hepato- and nephro-toxicity in rats
- Effects of peripherally and centrally applied ghrelin on the oxidative stress induced by renin angiotensin system in a rat model of renovascular hypertension
- Investigation of the role of α-lipoic acid on fatty acids profile, some minerals (zinc, copper, iron) and antioxidant activity against aluminum-induced oxidative stress in the liver of male rats
- Metabolism
- The choice of freely preferred cadence by trained nonprofessional cyclists may not be characterized by mechanical efficiency
- Immune Response
- Ascorbic acid does not modulate potassium currents in cultured human lymphocytes
- Phytotherapy
- Anti-inflammatory activity of Elaeagnus angustifolia fruit extract on rat paw edema
- Histopathological and biochemical assessments of Costus afer stem on alloxan-induced diabetic rats
- In vitro inhibition of phosphodiesterase-5 and arginase activities from rat penile tissue by two Nigerian herbs (Hunteria umbellata and Anogeissus leiocarpus)
- Antioxidant and antiproliferative potentials of methanol extract of Xylopia aethiopica (Dunal) A. Rich in PC-3 and LNCaP cells
