Neurobehavioural evaluation of Lophira alata (Ochnaceae) stem bark extract in mice
-
Loretta O. Iniaghe
,
Igbe Ighodaro
Abstract
Background: Stem bark and leaves of Lophira alata (Family: Ochnaceae) have been used traditionally for their anti-psychotic, anti-convulsant and anxiolytic properties. Since no existing data was found on the neurobehavioural properties, this study was carried out to evaluate some neurobehavioural properties of the aqueous extract of the stem bark of L. alata in animal models.
Methods: The oral mean lethal dose (LD50) of the extract was estimated, and preliminary phytochemical screening was conducted. Lophira alata extract (200, 400 and 800 mg/kg, p.o.) was investigated for antidepressant effect using the forced swim and tail suspension tests, and the anxiolytic potential was assessed using the stair case and hole board tests. Pentylenetetrazole-induced convulsion test was used to investigate the anticonvulsant potential of the extract.
Results: The LD50 was estimated to be >5000 mg/kg. Oral administration of L. alata extract produced a significant (p<0.05) non-dose-dependent decrease in the period of immobility in both the forced swim and tail suspension tests. While a significant decrease (p<0.05) in episodes of grooming was recorded in the staircase test, the number of head dips was not significantly reduced (p>0.05) in the hole board test. In the pentylenetetrazole-induced convulsion, a non-dose-dependent increase in onset of tonic-clonic seizures and protection from death was recorded.
Conclusions: The results obtained suggest that the aqueous stem bark extract of L. alata possesses neurobehavioural properties which may account for its use in ethnomedicine.
Acknowledgments
The authors are grateful to Dr. M.A.O Akanmu of Obafemi Awolowo University, Ile-Ife, for his professional insight, guidance and kind assistance through the design and execution of this study.
Author contributions: The study was designed by LOI and MGL, carried out by LOI, II, TPT and ITM. The manuscript was prepared by LOI, MGL and II. All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
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©2015 by De Gruyter
Artikel in diesem Heft
- Frontmatter
- Behavior and Neuroprotection
- Neurobehavioural evaluation of Lophira alata (Ochnaceae) stem bark extract in mice
- Cardiovascular Function
- Altered cardiovascular autonomic function in young normotensive offspring of hypertensive parents – Is obesity an additional risk factor?
- Atheromic and lymphoplasmacytic effects of Mangifera indica methanolic leaf extract on the heart of chinchilla rabbits
- Cardiovascular-Pulmonary Interactions
- Prevalence of cardiovascular risk factors and management practices of acute coronary syndrome in a tertiary care hospital
- Oxidative Stress
- Effect of homopterocarpin, an isoflavonoid from Pterocarpuserinaceus, on indices of liver injury and oxidative stress in acetaminophen-provoked hepatotoxicity
- Metabolism
- Activation of insulin signaling and energy sensing network by AICAR, an AMPK activator in insulin resistant rat tissues
- Mature coconut water exhibits antidiabetic and antithrombotic potential via L-arginine-nitric oxide pathway in alloxan induced diabetic rats
- Methanolic extract of Moringa oleifera leaves improves glucose tolerance, glycogen synthesis and lipid metabolism in alloxan-induced diabetic rats
- Morphological changes in the pancreas and glucose reduction of the aqueous extract of Costus afer leaf on alloxan-induced diabetic rats
- Immune Response
- Immunomodulatory activity of methanolic leaf extract of Moringa oleifera in Wistar albino rats
- Immune response modulatory effect of Emilia sonchifolia (L.) DC: an in vivo experimental study
- Inflammation
- Thymoquinone ameliorates NLRP3-mediated inflammation in the pancreas of albino Wistar rats fed ethanol and high-fat diet
- Miscellaneous
- Perceptions and practices of self-medication in healthcare and nonhealthcare university students in South India
Artikel in diesem Heft
- Frontmatter
- Behavior and Neuroprotection
- Neurobehavioural evaluation of Lophira alata (Ochnaceae) stem bark extract in mice
- Cardiovascular Function
- Altered cardiovascular autonomic function in young normotensive offspring of hypertensive parents – Is obesity an additional risk factor?
- Atheromic and lymphoplasmacytic effects of Mangifera indica methanolic leaf extract on the heart of chinchilla rabbits
- Cardiovascular-Pulmonary Interactions
- Prevalence of cardiovascular risk factors and management practices of acute coronary syndrome in a tertiary care hospital
- Oxidative Stress
- Effect of homopterocarpin, an isoflavonoid from Pterocarpuserinaceus, on indices of liver injury and oxidative stress in acetaminophen-provoked hepatotoxicity
- Metabolism
- Activation of insulin signaling and energy sensing network by AICAR, an AMPK activator in insulin resistant rat tissues
- Mature coconut water exhibits antidiabetic and antithrombotic potential via L-arginine-nitric oxide pathway in alloxan induced diabetic rats
- Methanolic extract of Moringa oleifera leaves improves glucose tolerance, glycogen synthesis and lipid metabolism in alloxan-induced diabetic rats
- Morphological changes in the pancreas and glucose reduction of the aqueous extract of Costus afer leaf on alloxan-induced diabetic rats
- Immune Response
- Immunomodulatory activity of methanolic leaf extract of Moringa oleifera in Wistar albino rats
- Immune response modulatory effect of Emilia sonchifolia (L.) DC: an in vivo experimental study
- Inflammation
- Thymoquinone ameliorates NLRP3-mediated inflammation in the pancreas of albino Wistar rats fed ethanol and high-fat diet
- Miscellaneous
- Perceptions and practices of self-medication in healthcare and nonhealthcare university students in South India
