Startseite Hepatoprotective role of kaempferol during alcohol- and ΔPUFA-induced oxidative stress
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Hepatoprotective role of kaempferol during alcohol- and ΔPUFA-induced oxidative stress

  • Garima Shakya , Selva Manjini , Muddasarul Hoda und Rukkumani Rajagopalan EMAIL logo
Veröffentlicht/Copyright: 24. Juli 2013

Abstract

Background: The present study aimed to analyze the effect of kaempferol on oxidative stress induced by alcohol and thermally oxidized polyunsaturated fatty acid (ΔPUFA) in male albino Wistar rats.

Methods: The rats were divided into four groups. The animals in group 1 served as the normal group (standard diet), group 2 served as the hepatotoxic group (alcohol+ΔPUFA), group 3 served as the treated group (alcohol+ΔPUFA+kaempferol), and group 4 served as kaempferol control. The levels of marker enzyme γ-glutamyl transferase (GGT), lipid peroxidation markers [thiobarbituric acid reactive substances (TBARS) and lipid hydroperoxides (LH)], enzymatic antioxidants (catalase, superoxide dismutase, and glutathione peroxidase), and nonenzymatic antioxidants (reduced glutathione, vitamin E, and vitamin C) were analyzed in liver to evaluate the effects of kaempferol.

Results: The levels of GGT, TBARS, and LH were significantly increased in liver of the alcohol+ΔPUFA group and were found to be reduced on treatment with kaempferol. The levels of both enzymatic and nonenzymatic antioxidants were decreased in liver of the alcohol+ΔPUFA group and were found to be restored on treatment with kaempferol.

Conclusions: From the results obtained, we conclude that kaempferol protects the liver against alcohol- and ΔPUFA- induced oxidative stress.


Corresponding author: Dr. Rukkumani Rajagopalan, Assistant Professor, Department of Biochemistry and Molecular Biology, School of Life Sciences, Pondicherry University, Puducherry 605014, India, Phone: +91 413-2654537, +91 9677847337, E-mail: ;

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Received: 2013-4-19
Accepted: 2013-7-1
Published Online: 2013-07-24
Published in Print: 2014-02-01

©2014 by Walter de Gruyter Berlin Boston

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