Evaluation of antiulcerogenic potential of antioxidant α-tocopherol in pylorus-ligated albino rats
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Shobha V. Huilgol
und
V. Hemanth Kumar
Abstract
Background: Oxidative free radicals and lipid peroxidation mediate gastric injury. α-Tocopherol is a redox agent with biological and antioxidant property, hence, may provide ulcer protection.
Methods: Pylorus-ligated Shay rats (n=6) were used as the experimental gastric ulcer animal model. The rats were divided into three groups. Group I received saline (5 mL/kg), Group II α-tocopherol (12.5 mg/kg), and Group III omeprazole (3.6 mg/kg), orally daily for 5 days prior to ulcerogenic challenge. Nineteen hours after the challenge, the rats were sacrificed and their stomachs isolated and studied for degree of gastric injury. Formed gastric juice was collected for measurement of volume, titrimetric estimation of free and total acidity, and total acid output by the conventional methods. The ulcer index and total acid outputs were calculated.
Results: α-Tocopherol exerted significant (p<0.05) antiulcer activity (the ulcer index was reduced to 7.4±1.0 from the control value of 19.8±4.1). α-Tocopherol also significantly reduced free and total acidity, gastric juice volume, and total acid output (p<0.01). The results were analyzed by ANOVA and Scheffe’s multiple comparison test.
Conclusions: The study demonstrates that α-tocopherol has significant antiulcer activity. It, perhaps, acts by decreasing hydrochloric acid output.
The authors are thankful to Ranbaxy Co. Ltd for providing gift samples of the drugs α-tocopherol and omeprazole.
Conflict of interest statement
Authors’ conflict of interest disclosure: The authors stated that there are no conflicts of interest regarding the publication of this article. Research funding played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
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- Masthead
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- The relationship between oxidative stress and exercise
- Original articles
- Testosterone promotes glucose intolerance, lipid disorder and oxidative stress in type 1 diabetic rats
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- Hepatoprotective role of kaempferol during alcohol- and ΔPUFA-induced oxidative stress
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- Comparison of the penetration and passage of Streptococcus mutans and Aggregatibacter actinomycetemcomitans through membranes loaded with tetracycline, amoxicillin, and chlorhexidine: an in vitro study
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