Comparative Studies on Physicochemical Properties of Bovine Serum Albumin–Glucose and Galactose Conjugates Formed by Glycation Combined with Ultrasonic Pretreatment
Abstract
The effects of ultrasonication on the physicochemical properties of bovine serum albumin (BSA)–glucose and the galactose conjugates formed by glycation was investigated. Fourier transform ion cyclotron resonance mass spectra analysis showed that the conjugates had a higher molecular weight than the native and ultrasonicated BSA. Ultrasonicated BSA had significantly higher emulsifying and foaming properties than native BSA. The browning intensity, surface hydrophobicity, emulsifying property and foaming capacity of the conjugates were substantially improved while the free amino groups, intrinsic fluorescence emission and foaming stability were decreased compared to native and ultrasonicated BSA. The results of this study indicate that ultrasonication is an efficient technique to improve the physicochemical properties of proteins. The glycation between ultrasonicated BSA and monosaccharide can effectively improve the physicochemical properties of BSA, and the glycation rate order is galactose \gt glucose. It also shows the critical role of monosaccharide conformational changes in improving the glycation and physicochemical properties of proteins.
Funding statement: China Scholarship Council, (Grant / Award Number: ‘201608360115’) Chinese National Natural Science Foundation, (Grant / Award Number: ‘ 31460395’)
Acknowledgements
This work was supported by funds from Chinese National Natural Science Foundation (No. 31460395), China Agriculture Research System (CARS-45) and China Scholar council (201608360115).
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Articles in the same Issue
- Research articles
- Effect of Inulin DP on Various Properties of Sugar-Free Dark Chocolates Containing Lactobacillus paracasei and Lactobacillus acidophilus
- Comparative Studies on Physicochemical Properties of Bovine Serum Albumin–Glucose and Galactose Conjugates Formed by Glycation Combined with Ultrasonic Pretreatment
- Simplified Physical Upgrading of High-Acid Adlay Bran Ethanol Extracts by Supercritical CO2
- Moisture Transport Mechanism and Drying Kinetic of Fresh Harvested Red Onion Bulbs under Dehumidified Air
- Dynamic Water Mobility in Sea Cucumber (Stichopus japonicas) During Drying Process Assessed by LF-NMR and MRI in situ
- Effect of Addition of Antioxidant Flaxseed Polypeptide on the Rheological Properties of Native Maize Starch
- Assessment of Ferrous Glycinate Liposome Absorption Using in Situ Single-Pass Perfusion Model
- The Immunoreactive Protein was Produced During Absorption of Glycinin or its Hydrolysate in IPEC-J2
- Microencapsulation of Bioactive Compounds from Blackberry Pomace (Rubus fruticosus) by Spray Drying Technique
- Shorter communications
- Flow Analysis for a Flow Channel Instrument to Evaluate Viscosities of Non-Newtonian Viscoelastic Liquid Foods