The Immunoreactive Protein was Produced During Absorption of Glycinin or its Hydrolysate in IPEC-J2
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Yuan Zhao
Abstract
The allergens absorbed in immunoreactive form by the gut epithelium might induce the occurrence of allergy. The purpose of this study was to investigate the absorption and intracellular accumulation of the intact or hydrolyzed glycinin in the porcine intestinal epithelial cells (IPEC-J2). The IPEC-J2 cells were incubated by 0, 0.25, 0.5 and 1.0 mg/mL glycinin or its hydrolysate for 2, 4, 8 or 12 h. The amounts of immunoreactive glycinin were measured by enzyme-linked immunosorbent assay. The intact and hydrolyzed glycinin fragments of epithelial absorption were identified by immunoblotting and mass spectrometry (MS). We found that glycinin or its hydrolysate is expensively absorbed with the increase of dose and time. The 35 kD or 22 kD protein with glycinin-specific epitopes was detected in the intracellular extracts and basolateral solutions. The results indicate that the glycinin or its hydrolysate could be absorbed; meanwhile, the 35kD or 22kD protein was correspondingly produced during absorption.
Funding statement: This work was financially supported by the National Natural Science Foundation of China (No. 31572439 and No.31572415), the Natural Science Foundation of Jilin Province of China (No. 20160101348JC), and “Twelfth Five-year Plan” for Sci & Tech Research Program of Jilin Education Department of P.R. China（No.2015198）
Conflict of Interest: The authors declare no conflict of interest.
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Articles in the same Issue
- Research articles
- Effect of Inulin DP on Various Properties of Sugar-Free Dark Chocolates Containing Lactobacillus paracasei and Lactobacillus acidophilus
- Comparative Studies on Physicochemical Properties of Bovine Serum Albumin–Glucose and Galactose Conjugates Formed by Glycation Combined with Ultrasonic Pretreatment
- Simplified Physical Upgrading of High-Acid Adlay Bran Ethanol Extracts by Supercritical CO2
- Moisture Transport Mechanism and Drying Kinetic of Fresh Harvested Red Onion Bulbs under Dehumidified Air
- Dynamic Water Mobility in Sea Cucumber (Stichopus japonicas) During Drying Process Assessed by LF-NMR and MRI in situ
- Effect of Addition of Antioxidant Flaxseed Polypeptide on the Rheological Properties of Native Maize Starch
- Assessment of Ferrous Glycinate Liposome Absorption Using in Situ Single-Pass Perfusion Model
- The Immunoreactive Protein was Produced During Absorption of Glycinin or its Hydrolysate in IPEC-J2
- Microencapsulation of Bioactive Compounds from Blackberry Pomace (Rubus fruticosus) by Spray Drying Technique
- Shorter communications
- Flow Analysis for a Flow Channel Instrument to Evaluate Viscosities of Non-Newtonian Viscoelastic Liquid Foods
