Startseite Lebenswissenschaften Altered population activity and local tuning heterogeneity in auditory cortex of Cacna2d3-deficient mice
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Altered population activity and local tuning heterogeneity in auditory cortex of Cacna2d3-deficient mice

  • Simon L. Wadle , Tatjana T.X. Schmitt , Jutta Engel , Simone Kurt und Jan J. Hirtz ORCID logo EMAIL logo
Veröffentlicht/Copyright: 8. November 2022
Biological Chemistry
Aus der Zeitschrift Biological Chemistry Band 404 Heft 6

Abstract

The α2δ3 auxiliary subunit of voltage-activated calcium channels is required for normal synaptic transmission and precise temporal processing of sounds in the auditory brainstem. In mice its loss additionally leads to an inability to distinguish amplitude-modulated tones. Furthermore, loss of function of α2δ3 has been associated with autism spectrum disorder in humans. To investigate possible alterations of network activity in the higher-order auditory system in α2δ3 knockout mice, we analyzed neuronal activity patterns and topography of frequency tuning within networks of the auditory cortex (AC) using two-photon Ca2+ imaging. Compared to wild-type mice we found distinct subfield-specific alterations in the primary auditory cortex, expressed in overall lower correlations between the network activity patterns in response to different sounds as well as lower reliability of these patterns upon repetitions of the same sound. Higher AC subfields did not display these alterations but showed a higher amount of well-tuned neurons along with lower local heterogeneity of the neurons’ frequency tuning. Our results provide new insight into AC network activity alterations in an autism spectrum disorder-associated mouse model.

Keywords: auditory processing deficits; autism spectrum disorder; GCaMP7f; in vivo Ca2+ imaging; voltage-gated calcium channels
Corresponding author: Jan J. Hirtz, Physiology of Neuronal Networks, Department of Biology, University of Kaiserslautern, Erwin-Schrödinger-Straße 13, D-67663 Kaiserslautern, Germany, E-mail:

Funding source: Deutsche Forschungsgemeinschaft

Award Identifier / Grant number: PP1608 (En 294/5-2 to JE, KU 1972/5-2 to SK) and SFB 894 (A8 to JE)

Funding source: BioComp Research Initiative

Acknowledgments

We thank Matthias Göttsche (Stocksee, Germany) for permitting the use of the recordings of the Blasius’s Horseshoe Bat. We thank Kerstin Fischer (Saarland University) and Kornelia Ociepka (University of Kaiserslautern) for excellent technical assistance.

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: This work was supported by Deutsche Forschungsgemeinschaft (DFG) PP1608 (En 294/5-2 to JE, KU 1972/5-2 to SK) and DFG SFB 894 (A8 to JE). We thank the BioComp Research Initiative for funding.

  3. Conflict of interest statement: The authors declare no conflicts of interest regarding this article.

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Supplementary Material

The online version of this article offers supplementary material (https://doi.org/10.1515/hsz-2022-0269).

Received: 2022-09-01
Accepted: 2022-10-24
Published Online: 2022-11-08
Published in Print: 2023-05-25

© 2022 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/hsz-2022-0269
Schlagwörter für diesen Artikel
auditory processing deficits; autism spectrum disorder; GCaMP7f; in vivo Ca2+ imaging; voltage-gated calcium channels

Artikel in diesem Heft

  1. Frontmatter
  2. Reviews
  3. Unravelling the genetic links between Parkinson’s disease and lung cancer
  4. Small-molecule metabolites in SARS-CoV-2 treatment: a comprehensive review
  5. Research Articles/Short Communications
  6. Molecular Medicine
  7. Dynamic regulation of eEF1A1 acetylation affects colorectal carcinogenesis
  8. New lipophilic organic nitrates: candidates for chronic skin disease therapy
  9. Cell Biology and Signaling
  10. Altered population activity and local tuning heterogeneity in auditory cortex of Cacna2d3-deficient mice
  11. Inhibition of miR-143-3p alleviates myocardial ischemia reperfusion injury via limiting mitochondria-mediated apoptosis
  12. Proteolysis
  13. The Mycobacterium tuberculosis prolyl dipeptidyl peptidase cleaves the N-terminal peptide of the immunoprotein CXCL-10
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