Advanced glycation endproducts and polysialylation affect the turnover of the neural cell adhesion molecule (NCAM) and the receptor for advanced glycation endproducts (RAGE)
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Franziska Frank
Abstract
The balance between protein synthesis and degradation regulates the amount of expressed proteins. This protein turnover is usually quantified as the protein half-life time. Several studies suggest that protein degradation decreases with age and leads to increased deposits of damaged and non-functional proteins. Glycation is an age-dependent, non-enzymatic process leading to posttranslational modifications, so-called advanced glycation endproducts (AGE), which usually damage proteins and lead to protein aggregation. AGE are formed by the Maillard reaction, where carbonyls of carbohydrates or metabolites react with amino groups of proteins. In this study, we quantified the half-life time of two important receptors of the immunoglobulin superfamily, the neural cell adhesion molecule (NCAM) and the receptor for advanced glycation end products (RAGE) before and after glycation. We found, that in two rat PC12 cell lines glycation leads to increased turnover, meaning that glycated, AGE-modified proteins are degraded faster than non-glycated proteins. NCAM is the most prominent carrier of a unique enzymatic posttranslational modification, the polysialylation. Using two PC12 cell lines (a non-polysialylated and a polysialylated one), we could additionally demonstrate, that polysialylation of NCAM has an impact on its turnover and that it significantly increases its half-life time.
Acknowledgement
This work was supported by the Deutsche Forschungsgemeinschaft, Funder Id: 10.13039/501100001659 (RTG 2155, ProMoAge).
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©2019 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- Mitochondria, Apoptosis and Cancer (MAC) 2017
- Transglutaminase type 2 in the regulation of proteostasis
- Mitochondria-driven elimination of cancer and senescent cells
- Comparative study of the differential cell death protecting effect of various ROS scavengers
- Involvement of mitophagy in cisplatin-induced cell death regulation
- Differential involvement of TAK1, RIPK1 and NF-κB signaling in Smac mimetic-induced cell death in breast cancer cells
- Selective BH3-mimetics targeting BCL-2, BCL-XL or MCL-1 induce severe mitochondrial perturbations
- BNIP3 contributes to the glutamine-driven aggressive behavior of melanoma cells
- Review
- Multiple binding sites in organic cation transporters require sophisticated procedures to identify interactions of novel drugs
- Research Articles/Short Communications
- Membranes, Lipids, Glycobiology
- Characterization of the cholesterol efflux of apolipoprotein E-containing high-density lipoprotein in THP-1 cells
- Advanced glycation endproducts and polysialylation affect the turnover of the neural cell adhesion molecule (NCAM) and the receptor for advanced glycation endproducts (RAGE)
- Cell Biology and Signaling
- miR-34a-5p aggravates hypoxia-induced apoptosis by targeting ZEB1 in cardiomyocytes
- MicroRNA-1225-5p acts as a tumor-suppressor in laryngeal cancer via targeting CDC14B
- Novel Techniques
- Tandem DNAzyme for double digestion: a new tool for circRNA suppression
Artikel in diesem Heft
- Frontmatter
- Mitochondria, Apoptosis and Cancer (MAC) 2017
- Transglutaminase type 2 in the regulation of proteostasis
- Mitochondria-driven elimination of cancer and senescent cells
- Comparative study of the differential cell death protecting effect of various ROS scavengers
- Involvement of mitophagy in cisplatin-induced cell death regulation
- Differential involvement of TAK1, RIPK1 and NF-κB signaling in Smac mimetic-induced cell death in breast cancer cells
- Selective BH3-mimetics targeting BCL-2, BCL-XL or MCL-1 induce severe mitochondrial perturbations
- BNIP3 contributes to the glutamine-driven aggressive behavior of melanoma cells
- Review
- Multiple binding sites in organic cation transporters require sophisticated procedures to identify interactions of novel drugs
- Research Articles/Short Communications
- Membranes, Lipids, Glycobiology
- Characterization of the cholesterol efflux of apolipoprotein E-containing high-density lipoprotein in THP-1 cells
- Advanced glycation endproducts and polysialylation affect the turnover of the neural cell adhesion molecule (NCAM) and the receptor for advanced glycation endproducts (RAGE)
- Cell Biology and Signaling
- miR-34a-5p aggravates hypoxia-induced apoptosis by targeting ZEB1 in cardiomyocytes
- MicroRNA-1225-5p acts as a tumor-suppressor in laryngeal cancer via targeting CDC14B
- Novel Techniques
- Tandem DNAzyme for double digestion: a new tool for circRNA suppression
