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Comparative study of the differential cell death protecting effect of various ROS scavengers

  • Nežka Kavčič , Katarina Pegan , Peter Vandenabeele and Boris Turk EMAIL logo
Published/Copyright: July 24, 2018
Biological Chemistry
From the journal Biological Chemistry Volume 400 Issue 2

Abstract

Oxidative stress has for a long time been associated with cell death, especially classical necrosis, however, its role in other cell death pathways is less clear. Here, we evaluated in a comparative way, the effect of four different reactive oxygen species (ROS) scavengers, N-acetyl-L-cysteine (NAC), α-tocopherol and two superoxide dismutase mimetics, n(III)tetrakis(4-benzoic acid)porphyrin chloride, and 4-hydroxy-2,2,6,6-tetramethylpiperidine 1-oxyl (Tempol), in four different cell death models, including menadione-triggered necrosis, staurosporine-induced apoptosis and tumor necrosis factor (TNF)-induced apoptosis and necroptosis. While menadione-triggered necrosis was completely prevented by the classical ROS scavenger NAC and to a substantial amount by the other scavengers, ROS targeting was found to have a marginal effect on the other cell death modalities investigated. Despite its side-effects at higher concentrations, Tempol was able to substantially prevent TNF-induced apoptosis and to a somewhat lesser extent TNF-induced necroptosis. However, this seems to be separated from its ROS-scavenging function.

Keywords: apoptosis; cell death; necroptosis; necrosis; ROS; ROS scavenger; Tempol

Acknowledgments

B.T.’s research was supported by the grants from Slovene Research Agency (P1-0140 and N1-0022), whereas P.V.’s research was supported by Belgian grants (Interuniversity Attraction Poles, IAP 7/32), Flemish grants (Research Foundation Flanders: FWO G.0C37.14N, FWO G.0E04.16N), Methusalem grant (BOF16/MET_V/007), a grant from the ‘Foundation against Cancer’ (FAF-F/2016/865), CRIG and VIB.

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Supplementary Material

The online version of this article offers supplementary material (https://doi.org/10.1515/hsz-2017-0317).

Received: 2017-12-19
Accepted: 2018-07-05
Published Online: 2018-07-24
Published in Print: 2019-01-28

©2019 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/hsz-2017-0317
Keywords for this article
apoptosis; cell death; necroptosis; necrosis; ROS; ROS scavenger; Tempol

Articles in the same Issue

  1. Frontmatter
  2. Mitochondria, Apoptosis and Cancer (MAC) 2017
  3. Transglutaminase type 2 in the regulation of proteostasis
  4. Mitochondria-driven elimination of cancer and senescent cells
  5. Comparative study of the differential cell death protecting effect of various ROS scavengers
  6. Involvement of mitophagy in cisplatin-induced cell death regulation
  7. Differential involvement of TAK1, RIPK1 and NF-κB signaling in Smac mimetic-induced cell death in breast cancer cells
  8. Selective BH3-mimetics targeting BCL-2, BCL-XL or MCL-1 induce severe mitochondrial perturbations
  9. BNIP3 contributes to the glutamine-driven aggressive behavior of melanoma cells
  10. Review
  11. Multiple binding sites in organic cation transporters require sophisticated procedures to identify interactions of novel drugs
  12. Research Articles/Short Communications
  13. Membranes, Lipids, Glycobiology
  14. Characterization of the cholesterol efflux of apolipoprotein E-containing high-density lipoprotein in THP-1 cells
  15. Advanced glycation endproducts and polysialylation affect the turnover of the neural cell adhesion molecule (NCAM) and the receptor for advanced glycation endproducts (RAGE)
  16. Cell Biology and Signaling
  17. miR-34a-5p aggravates hypoxia-induced apoptosis by targeting ZEB1 in cardiomyocytes
  18. MicroRNA-1225-5p acts as a tumor-suppressor in laryngeal cancer via targeting CDC14B
  19. Novel Techniques
  20. Tandem DNAzyme for double digestion: a new tool for circRNA suppression
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