Abstract
High-density lipoprotein (HDL), also known as antiatherogenic lipoprotein, consists of heterogeneous particles in terms of size, density and composition, suggesting differences among HDL subclasses in characteristics and functions. We investigated the role of apolipoprotein E (apoE)-containing HDL, a minor HDL subclass, in the cholesterol efflux capacity (CEC) of HDL, which is its predominant atheroprotective function. The CEC of apoE-containing HDL was similar to that of apoE-deficient HDL, but the former exhibited a greater rate increase (1.48-fold) compared to that of the latter (1.10-fold) by the stimulation of THP-1 macrophages with the Liver X Receptor (LXR) agonist. No difference in CEC was observed without the LXR agonist between apoA-I, the main apolipoprotein in HDL, and apoE, whereas the increase in CEC in response to treatment with the LXR agonist was greater for apoA-I (4.25-fold) than for apoE (2.22-fold). Furthermore, the increase in the CEC of apoE-containing HDL induced by the LXR agonist was significantly reduced by treatment with glyburide, an inhibitor of ATP-binding cassette transporter A1 (ABCA1). These results suggest that apoE-containing HDL, unlike apoE-deficient HDL, is involved in cholesterol efflux via ABCA1.
Acknowledgments
This research was supported in part by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (Funder Id: 10.13039/501100001691, grant number 17K15771, to R.O., and grant number 26460642, to M.T.) and a grant from the Hokuto Foundation for Bioscience to R.O.
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©2019 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Mitochondria, Apoptosis and Cancer (MAC) 2017
- Transglutaminase type 2 in the regulation of proteostasis
- Mitochondria-driven elimination of cancer and senescent cells
- Comparative study of the differential cell death protecting effect of various ROS scavengers
- Involvement of mitophagy in cisplatin-induced cell death regulation
- Differential involvement of TAK1, RIPK1 and NF-κB signaling in Smac mimetic-induced cell death in breast cancer cells
- Selective BH3-mimetics targeting BCL-2, BCL-XL or MCL-1 induce severe mitochondrial perturbations
- BNIP3 contributes to the glutamine-driven aggressive behavior of melanoma cells
- Review
- Multiple binding sites in organic cation transporters require sophisticated procedures to identify interactions of novel drugs
- Research Articles/Short Communications
- Membranes, Lipids, Glycobiology
- Characterization of the cholesterol efflux of apolipoprotein E-containing high-density lipoprotein in THP-1 cells
- Advanced glycation endproducts and polysialylation affect the turnover of the neural cell adhesion molecule (NCAM) and the receptor for advanced glycation endproducts (RAGE)
- Cell Biology and Signaling
- miR-34a-5p aggravates hypoxia-induced apoptosis by targeting ZEB1 in cardiomyocytes
- MicroRNA-1225-5p acts as a tumor-suppressor in laryngeal cancer via targeting CDC14B
- Novel Techniques
- Tandem DNAzyme for double digestion: a new tool for circRNA suppression
Articles in the same Issue
- Frontmatter
- Mitochondria, Apoptosis and Cancer (MAC) 2017
- Transglutaminase type 2 in the regulation of proteostasis
- Mitochondria-driven elimination of cancer and senescent cells
- Comparative study of the differential cell death protecting effect of various ROS scavengers
- Involvement of mitophagy in cisplatin-induced cell death regulation
- Differential involvement of TAK1, RIPK1 and NF-κB signaling in Smac mimetic-induced cell death in breast cancer cells
- Selective BH3-mimetics targeting BCL-2, BCL-XL or MCL-1 induce severe mitochondrial perturbations
- BNIP3 contributes to the glutamine-driven aggressive behavior of melanoma cells
- Review
- Multiple binding sites in organic cation transporters require sophisticated procedures to identify interactions of novel drugs
- Research Articles/Short Communications
- Membranes, Lipids, Glycobiology
- Characterization of the cholesterol efflux of apolipoprotein E-containing high-density lipoprotein in THP-1 cells
- Advanced glycation endproducts and polysialylation affect the turnover of the neural cell adhesion molecule (NCAM) and the receptor for advanced glycation endproducts (RAGE)
- Cell Biology and Signaling
- miR-34a-5p aggravates hypoxia-induced apoptosis by targeting ZEB1 in cardiomyocytes
- MicroRNA-1225-5p acts as a tumor-suppressor in laryngeal cancer via targeting CDC14B
- Novel Techniques
- Tandem DNAzyme for double digestion: a new tool for circRNA suppression