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Characterization of the cholesterol efflux of apolipoprotein E-containing high-density lipoprotein in THP-1 cells

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Published/Copyright: September 13, 2018

Abstract

High-density lipoprotein (HDL), also known as antiatherogenic lipoprotein, consists of heterogeneous particles in terms of size, density and composition, suggesting differences among HDL subclasses in characteristics and functions. We investigated the role of apolipoprotein E (apoE)-containing HDL, a minor HDL subclass, in the cholesterol efflux capacity (CEC) of HDL, which is its predominant atheroprotective function. The CEC of apoE-containing HDL was similar to that of apoE-deficient HDL, but the former exhibited a greater rate increase (1.48-fold) compared to that of the latter (1.10-fold) by the stimulation of THP-1 macrophages with the Liver X Receptor (LXR) agonist. No difference in CEC was observed without the LXR agonist between apoA-I, the main apolipoprotein in HDL, and apoE, whereas the increase in CEC in response to treatment with the LXR agonist was greater for apoA-I (4.25-fold) than for apoE (2.22-fold). Furthermore, the increase in the CEC of apoE-containing HDL induced by the LXR agonist was significantly reduced by treatment with glyburide, an inhibitor of ATP-binding cassette transporter A1 (ABCA1). These results suggest that apoE-containing HDL, unlike apoE-deficient HDL, is involved in cholesterol efflux via ABCA1.

Acknowledgments

This research was supported in part by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (Funder Id: 10.13039/501100001691, grant number 17K15771, to R.O., and grant number 26460642, to M.T.) and a grant from the Hokuto Foundation for Bioscience to R.O.

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Received: 2018-06-12
Accepted: 2018-09-02
Published Online: 2018-09-13
Published in Print: 2019-01-28

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