MicroRNA-544 down-regulates both Bcl6 and Stat3 to inhibit tumor growth of human triple negative breast cancer
Abstract
Triple negative breast cancer lacking estrogen receptor (ER), progesterone receptor and Her2 account for account for the majority of the breast cancer deaths, due to the lack of specific gene targeted therapy. Our current study aimed to investigate the role of miR-544 in triple negative breast cancer. Endogenous levels of miR-544 were significantly lower in breast cancer cell lines than in human breast non-tumorigenic and mammary epithelial cell lines. We found that miR-544 directly targeted the 3′-untranslated region (UTR) on both Bcl6 and Stat3 mRNAs, and overexpression of miR-544 in triple negative breast cancer cells significantly down-regulated expressions of Bcl6 and Stat3, which in turn severely inhibited cancer cell proliferation, migration and invasion in vitro. Employing a mouse xenograft model to examine the in vivo function of miR-544, we found that expression of miR-544 significantly repressed the growth of xenograft tumors. Our current study reported miR-544 as a tumor-suppressor microRNA particularly in triple negative breast cancer. Our data supported the role of miR-544 as a potential biomarker in developing gene targeted therapies in the clinical treatment of triple negative breast cancer.
Acknowledgments
This work was supported by Anhui Provincial Department of Education (KJ2013Z214).
Conflict of interest statement: The authors have declared that no competing interests exist.
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The online version of this article (DOI: 10.1515/hsz-2016-0104) offers supplementary material, available to authorized users.
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Artikel in diesem Heft
- Frontmatter
- Guest Editorial
- Highlight: annexins in health and disease
- HIGHLIGHT: ANNEXINS IN HEALTH AND DISEASE
- Emerging functions as host cell factors – an encyclopedia of annexin-pathogen interactions
- Annexins in plasma membrane repair
- Annexin A1: shifting the balance towards resolution and repair
- Annexin A1 and resolution of inflammation: tissue repairing properties and signalling signature
- Annexins A2 and A8 in endothelial cell exocytosis and the control of vascular homeostasis
- The annexin A2 system and angiogenesis
- More than just innate affairs – on the role of annexins in adaptive immunity
- Annexins – insights from knockout mice
- Review
- Regulation of Rap GTPases in mammalian neurons
- Research Articles/Short Communications
- Protein Structure and Function
- Insights into K-Ras 4B regulation by post-translational lysine acetylation
- Cell Biology and Signaling
- MicroRNA-544 down-regulates both Bcl6 and Stat3 to inhibit tumor growth of human triple negative breast cancer
Artikel in diesem Heft
- Frontmatter
- Guest Editorial
- Highlight: annexins in health and disease
- HIGHLIGHT: ANNEXINS IN HEALTH AND DISEASE
- Emerging functions as host cell factors – an encyclopedia of annexin-pathogen interactions
- Annexins in plasma membrane repair
- Annexin A1: shifting the balance towards resolution and repair
- Annexin A1 and resolution of inflammation: tissue repairing properties and signalling signature
- Annexins A2 and A8 in endothelial cell exocytosis and the control of vascular homeostasis
- The annexin A2 system and angiogenesis
- More than just innate affairs – on the role of annexins in adaptive immunity
- Annexins – insights from knockout mice
- Review
- Regulation of Rap GTPases in mammalian neurons
- Research Articles/Short Communications
- Protein Structure and Function
- Insights into K-Ras 4B regulation by post-translational lysine acetylation
- Cell Biology and Signaling
- MicroRNA-544 down-regulates both Bcl6 and Stat3 to inhibit tumor growth of human triple negative breast cancer