Startseite Lithium chloride improves the efficiency of induced pluripotent stem cell-derived neurospheres
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Lithium chloride improves the efficiency of induced pluripotent stem cell-derived neurospheres

  • Azita Parvaneh Tafreshi EMAIL logo , Aude Sylvain , Guizhi Sun , Daniella Herszfeld , Keith Schulze und Claude C.A. Bernard
Veröffentlicht/Copyright: 14. März 2015

Abstract

Induced pluripotent stem cell (iPSC)-derived neurospheres, which consist mainly of neural progenitors, are considered to be a good source of neural cells for transplantation in regenerative medicine. In this study, we have used lithium chloride, which is known to be a neuroprotective agent, in an iPSC-derived neurosphere model, and examined both the formation rate and size of the neurospheres as well as the proliferative and apoptotic status of their contents. Our results showed that lithium enhanced the formation and the sizes of the iPSC-derived neurospheres, increased the number of Ki67-positive proliferating cells, but reduced the number of the TUNEL-positive apoptotic cells. This increased number of Ki67 proliferating cells was secondary to the decreased apoptosis and not to the stimulation of cell cycle entry, as the expression of the proliferation marker cyclin D1 mRNA did not change after lithium treatment. Altogether, we suggest that lithium enhances the survival of neural progenitors and thus the quality of the iPSC-derived neurospheres, which may strengthen the prospect of using lithium-treated pluripotent cells and their derivatives in a clinical setting.


Corresponding author: Azita Parvaneh Tafreshi, The National Research Institute of Genetic Engineering and Biotechnology, Faculty of Medical Biotechnology, Department of Molecular Medicine, Tehran, Iran 14965-161, e-mail: ;

Acknowledgments

This work was supported by a grant from the Iranian National Science Foundation (INSF) granted to Dr. Azita Parvaneh Tafreshi. CCAB is supported by grants from Victoria/CIRM Joint Project (RMI-01739) and the National Health and Medical Research Council of Australia/CIRM joint Project (APP1053621). Monash Microimaging Institute (MMI) is acknowledged for their provision of microscopy instrumentation and technical support. The authors declare no competing financial interests.

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Received: 2014-10-10
Accepted: 2015-2-17
Published Online: 2015-3-14
Published in Print: 2015-8-1

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