Home Life Sciences Effect of Artemisia absinthium ethanolic extract on oxidative stress markers and the TLR4, S100A4, Bax and Bcl-2 genes expression in the kidney of STZ-induced diabetic rats
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Effect of Artemisia absinthium ethanolic extract on oxidative stress markers and the TLR4, S100A4, Bax and Bcl-2 genes expression in the kidney of STZ-induced diabetic rats

  • Fatemeh Bagheri , Jamal Amri , Mehdi Salehi , Hadi Karami , Abbas Alimoradian and Seied Amirhossein Latifi EMAIL logo
Published/Copyright: October 21, 2020

Abstract

Objectives

The present study was conducted to examine antidiabetic effects of Artemisia absinthium ethanolic extract [A. absinthium] and to investigate its effects on oxidative stress markers and the expression of TLR4, S100A4, Bax and Bcl-2 genes in the kidney of STZ-induced diabetic rats.

Methods

Thirty six rats (weight 200–250 g) were randomly divided into diabetes and control groups. Induction of diabetes was performed using STZ (55 mg/kg.bw). Biochemical parameters and oxidative stress markers (SOD and MDA) were measured using spectrophotometry after 60 days of treatment. The expression of TLR4, S100A4, Bax and Bcl-2 were analyzed by real-time PCR. One-way analysis of variance (ANOVA) and Bonferroni post hoc test were used to compare the data.

Results

Diabetes significantly impairs the serum fasting blood glucose (FBG), lipid profile, urea, creatinine and albumin. At the end of treatment with A. absinthium extract, these parameters were close to the normal range. The results showed that the A. absinthium extract significantly decreased the kidney expression of TLR4, S100A4, Bax and increased the expression of Bcl-2 and improved oxidative stress markers (SOD and MDA) in the kidney tissues of treated rats. Also, all of these beneficial effects of the A. absinthium were dose-dependent.

Conclusions

The extract of A. absinthium possesses antidiabetic effects. A. absinthium decreased the expression of TLR4, S100A4, Bax and increased the expression of Bcl-2 and improved oxidative stress. Therefore, this herbal extract can be used as an adjuvant treatment for diabetic complications.


Corresponding author: Seied Amirhossein Latifi, Ph.D., Traditional and Complementary Medicine Research Center, Arak University of Medical Sciences, Arak, Islamic Republic of Iran, Phone: +98 8634173505 (294), Fax: +98 86 3417 3521, E-mail:

  1. Research funding: Authors state no funding involved.

  2. Author Contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  3. Competing interests: Authors state no conflict of interest.

  4. Informed consent: Not applicable.

  5. Ethical approval: Animal procedures were carried out considering the Guide for the Care and Use of Laboratory that approved by the Committee on Animal Research of Arak University of Medical Sciences. The grant number was 6067.

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Received: 2020-04-28
Accepted: 2020-09-10
Published Online: 2020-10-21

© 2020 Walter de Gruyter GmbH, Berlin/Boston

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