Effect of Artemisia absinthium ethanolic extract on oxidative stress markers and the TLR4, S100A4, Bax and Bcl-2 genes expression in the kidney of STZ-induced diabetic rats
-
Fatemeh Bagheri
, Jamal Amri , Mehdi Salehi , Hadi Karami , Abbas Alimoradian and Seied Amirhossein Latifi
Abstract
Objectives
The present study was conducted to examine antidiabetic effects of Artemisia absinthium ethanolic extract [A. absinthium] and to investigate its effects on oxidative stress markers and the expression of TLR4, S100A4, Bax and Bcl-2 genes in the kidney of STZ-induced diabetic rats.
Methods
Thirty six rats (weight 200–250 g) were randomly divided into diabetes and control groups. Induction of diabetes was performed using STZ (55 mg/kg.bw). Biochemical parameters and oxidative stress markers (SOD and MDA) were measured using spectrophotometry after 60 days of treatment. The expression of TLR4, S100A4, Bax and Bcl-2 were analyzed by real-time PCR. One-way analysis of variance (ANOVA) and Bonferroni post hoc test were used to compare the data.
Results
Diabetes significantly impairs the serum fasting blood glucose (FBG), lipid profile, urea, creatinine and albumin. At the end of treatment with A. absinthium extract, these parameters were close to the normal range. The results showed that the A. absinthium extract significantly decreased the kidney expression of TLR4, S100A4, Bax and increased the expression of Bcl-2 and improved oxidative stress markers (SOD and MDA) in the kidney tissues of treated rats. Also, all of these beneficial effects of the A. absinthium were dose-dependent.
Conclusions
The extract of A. absinthium possesses antidiabetic effects. A. absinthium decreased the expression of TLR4, S100A4, Bax and increased the expression of Bcl-2 and improved oxidative stress. Therefore, this herbal extract can be used as an adjuvant treatment for diabetic complications.
Research funding: Authors state no funding involved.
Author Contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
Competing interests: Authors state no conflict of interest.
Informed consent: Not applicable.
Ethical approval: Animal procedures were carried out considering the Guide for the Care and Use of Laboratory that approved by the Committee on Animal Research of Arak University of Medical Sciences. The grant number was 6067.
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© 2020 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Letter to the Editor
- Evidence-based medicine is a valuable tool for a successful decision-making on risk-reducing bilateral mastectomy
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- Melatonin inhibits high glucose-induced ox-LDL/LDL expression and apoptosis in human umbilical endothelial cells
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- Greater effects of high- compared with moderate-intensity interval training on thyroid hormones in overweight/obese adolescent girls
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- Female urine incontinence: vaginal erbium laser (VEL) effectiveness and safety
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Articles in the same Issue
- Letter to the Editor
- Evidence-based medicine is a valuable tool for a successful decision-making on risk-reducing bilateral mastectomy
- Original Articles
- Melatonin inhibits high glucose-induced ox-LDL/LDL expression and apoptosis in human umbilical endothelial cells
- Effects of metformin and insulin therapy regimens on postpartum oral glucose tolerance test results in pregnant women with gestational diabetes mellitus: a comparative study
- Association of IL-33 gene rs16924159 polymorphism and recurrent pregnancy loss in Iranian Azeri women
- Greater effects of high- compared with moderate-intensity interval training on thyroid hormones in overweight/obese adolescent girls
- Effect of Artemisia absinthium ethanolic extract on oxidative stress markers and the TLR4, S100A4, Bax and Bcl-2 genes expression in the kidney of STZ-induced diabetic rats
- Associations of endothelial biomarkers, nitric oxide metabolites and endothelin, with blood pressure and coronary lesions depend on cardiovascular risk and sex to mark endothelial dysfunction on the SCORE scale
- Association of nitric oxide synthase 3 gene rs1799983 G/T polymorphism with idiopathic asthenozoospermia in Iranian Azeri males: a case-control study
- Adiponectin and leptin levels in normal weight women with polycystic ovary syndrome
- Review Articles
- Association between vitamin D deficiency and prevalence of metabolic syndrome in female population: a systematic review
- Vitamin D and sleep duration: Is there a bidirectional relationship?
- Challenges of post-traumatic stress disorder (PTSD) in Iraq: biochemical network and methodologies. A brief review
- Female urine incontinence: vaginal erbium laser (VEL) effectiveness and safety
- Breast cancer risk factors in Iran: a systematic review & meta-analysis
- Effects of melatonin supplementation on oxidative stress: a systematic review and meta-analysis of randomized controlled trials