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Oxidative polymerisation of models for phenolic lignin end-groups by laccase
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Dimitri Areskogh
Veröffentlicht/Copyright:
4. Dezember 2009
Abstract
The redox enzyme laccase can lead to cross-linking of lignin molecules by oxidising phenolic end groups to resonance-stabilised radicals that can undergo radical coupling to form covalent bonds. This property has potential for many technical applications. However, laccase treatment can also lead to degradation. Experiments were performed with two laccases of different oxidation potential and pH and temperature optima. The predominant reaction following laccase oxidation is the formation of 5-5′ and 4-O-5′ bonds. If the 5-position is blocked, other reactions occur, including coupling of the 1-position and oxidation of the α-position, which aggravates cross-linking of different lignin molecules. The product profile generated by the two laccases is somewhat different, mainly because of the different pH rather than differences in enzyme activity. Reaction mechanisms and the technical and biological significance of the results are discussed.
Keywords: biotechnology; laccase; lignin; lignin model compound; lignin polymerisation; phenolic end group; reaction mechanism
Received: 2009-3-19
Accepted: 2009-6-26
Published Online: 2009-12-04
Published Online: 2009-12-07
Published in Print: 2010-01-01
©2010 by Walter de Gruyter Berlin New York
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Artikel in diesem Heft
- Original Papers
- Initiating ECF bleaching sequences of eucalyptus kraft pulps with Z/D and Z/E stages
- Characterization of black liquors from kraft pulping of first-thinning Scots pine (Pinus sylvestris L.)
- Analysis of products from the oxidation of technical lignins by oxygen and H3PMo12O40 in water and aqueous methanol by size-exclusion chromatography
- Oxidative polymerisation of models for phenolic lignin end-groups by laccase
- Determination of anionic groups in wood by time-of-flight secondary ion mass spectrometry and laser ablation-inductively coupled plasma-mass spectrometry
- Effects of sample preparation on NIR spectroscopic estimation of chemical properties of Eucalyptus urophylla S.T. Blake wood
- Detection of wet-pockets on the surface of Tsuga heterophylla (Raf.) Sarg. by near infrared (NIR) spectroscopy
- Cellular distribution of coniferin in differentiating xylem of Chamaecyparis obtusa as revealed by Raman microscopy
- Hydrolysis of cellulose and wood powder treated with DMDHEU by a hydrolase enzyme complex, Fenton's reagent, and in a liquid culture of Trametes versicolor
- Effects of timing and intensity of thinning on wood structure and chemistry in Norway spruce
- Effects of calcium-based materials and iron impurities on wood degradation by the brown rot fungus Serpula lacrymans
- Lignin and carbohydrate variation with earlywood, latewood, and compression wood content of bent and straight ramets of a radiata pine clone
- Tensile stress relaxation of wood impregnated with different ACQ formulations at various temperatures
- Estimate of resistance-curve in wood through the double cantilever beam test
- Moisture-induced stresses and distortions in spruce cross-laminates and composite laminates
- Short Note
- Recovery of cellulose and xylan liquefied in ionic liquids by precipitation in anti-solvents
- Meetings
- Meetings
Schlagwörter für diesen Artikel
biotechnology;
laccase;
lignin;
lignin model compound;
lignin polymerisation;
phenolic end group;
reaction mechanism
Artikel in diesem Heft
- Original Papers
- Initiating ECF bleaching sequences of eucalyptus kraft pulps with Z/D and Z/E stages
- Characterization of black liquors from kraft pulping of first-thinning Scots pine (Pinus sylvestris L.)
- Analysis of products from the oxidation of technical lignins by oxygen and H3PMo12O40 in water and aqueous methanol by size-exclusion chromatography
- Oxidative polymerisation of models for phenolic lignin end-groups by laccase
- Determination of anionic groups in wood by time-of-flight secondary ion mass spectrometry and laser ablation-inductively coupled plasma-mass spectrometry
- Effects of sample preparation on NIR spectroscopic estimation of chemical properties of Eucalyptus urophylla S.T. Blake wood
- Detection of wet-pockets on the surface of Tsuga heterophylla (Raf.) Sarg. by near infrared (NIR) spectroscopy
- Cellular distribution of coniferin in differentiating xylem of Chamaecyparis obtusa as revealed by Raman microscopy
- Hydrolysis of cellulose and wood powder treated with DMDHEU by a hydrolase enzyme complex, Fenton's reagent, and in a liquid culture of Trametes versicolor
- Effects of timing and intensity of thinning on wood structure and chemistry in Norway spruce
- Effects of calcium-based materials and iron impurities on wood degradation by the brown rot fungus Serpula lacrymans
- Lignin and carbohydrate variation with earlywood, latewood, and compression wood content of bent and straight ramets of a radiata pine clone
- Tensile stress relaxation of wood impregnated with different ACQ formulations at various temperatures
- Estimate of resistance-curve in wood through the double cantilever beam test
- Moisture-induced stresses and distortions in spruce cross-laminates and composite laminates
- Short Note
- Recovery of cellulose and xylan liquefied in ionic liquids by precipitation in anti-solvents
- Meetings
- Meetings
