Cellular distribution of coniferin in differentiating xylem of Chamaecyparis obtusa as revealed by Raman microscopy
-
Yohei Morikawa
Abstract
Cellular distribution of coniferin in differentiating xylem of Japanese cypress (Chamaecyparis obtusa) was analyzed by Raman microscopy. Small blocks were collected from differentiating xylem, frozen, cut on their surface with a sliding microtome, and then freeze-dried. Scanning electron microscopy showed numerous needle-like deposits in the tracheid lumina from the beginning of the S1 layer formation to the S2 layer-forming stage. The Raman spectrum of the deposits in the tracheid lumen was similar to that of coniferin. The presence of coniferin in a water extract from differentiating xylem was confirmed by matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy and 1H- and 13C-nuclear magnetic resonance spectra. Differential Raman spectra taken from samples before and after washing with water and dehydration in an ethanol showed that developing secondary walls contained coniferin during the S2 layer-forming stage and also after S3 layer formation. In contrast, coniferin was detected in the cell corner middle lamella during the S2 layer-forming stage, and the differential spectra were different from that of coniferin after S3 layer formation. The differential spectrum in this stage was similar to that of a dehydrogenation polymer of coniferyl alcohol prepared by the “zulauf” method (bulk polymerization). These results suggest that free lignin oligomers of the type bulk polymerizate might exist in the cell corner middle lamella during the S3 layer-forming stage and can be removed from specimens during washing and dehydration. The results can be interpreted in a way that no such oligomer exists in the secondary wall during the same stage owing to endwise addition of monolignols (in analogy to a “zutropf” polymerization).
©2010 by Walter de Gruyter Berlin New York
Articles in the same Issue
- Original Papers
- Initiating ECF bleaching sequences of eucalyptus kraft pulps with Z/D and Z/E stages
- Characterization of black liquors from kraft pulping of first-thinning Scots pine (Pinus sylvestris L.)
- Analysis of products from the oxidation of technical lignins by oxygen and H3PMo12O40 in water and aqueous methanol by size-exclusion chromatography
- Oxidative polymerisation of models for phenolic lignin end-groups by laccase
- Determination of anionic groups in wood by time-of-flight secondary ion mass spectrometry and laser ablation-inductively coupled plasma-mass spectrometry
- Effects of sample preparation on NIR spectroscopic estimation of chemical properties of Eucalyptus urophylla S.T. Blake wood
- Detection of wet-pockets on the surface of Tsuga heterophylla (Raf.) Sarg. by near infrared (NIR) spectroscopy
- Cellular distribution of coniferin in differentiating xylem of Chamaecyparis obtusa as revealed by Raman microscopy
- Hydrolysis of cellulose and wood powder treated with DMDHEU by a hydrolase enzyme complex, Fenton's reagent, and in a liquid culture of Trametes versicolor
- Effects of timing and intensity of thinning on wood structure and chemistry in Norway spruce
- Effects of calcium-based materials and iron impurities on wood degradation by the brown rot fungus Serpula lacrymans
- Lignin and carbohydrate variation with earlywood, latewood, and compression wood content of bent and straight ramets of a radiata pine clone
- Tensile stress relaxation of wood impregnated with different ACQ formulations at various temperatures
- Estimate of resistance-curve in wood through the double cantilever beam test
- Moisture-induced stresses and distortions in spruce cross-laminates and composite laminates
- Short Note
- Recovery of cellulose and xylan liquefied in ionic liquids by precipitation in anti-solvents
- Meetings
- Meetings
Articles in the same Issue
- Original Papers
- Initiating ECF bleaching sequences of eucalyptus kraft pulps with Z/D and Z/E stages
- Characterization of black liquors from kraft pulping of first-thinning Scots pine (Pinus sylvestris L.)
- Analysis of products from the oxidation of technical lignins by oxygen and H3PMo12O40 in water and aqueous methanol by size-exclusion chromatography
- Oxidative polymerisation of models for phenolic lignin end-groups by laccase
- Determination of anionic groups in wood by time-of-flight secondary ion mass spectrometry and laser ablation-inductively coupled plasma-mass spectrometry
- Effects of sample preparation on NIR spectroscopic estimation of chemical properties of Eucalyptus urophylla S.T. Blake wood
- Detection of wet-pockets on the surface of Tsuga heterophylla (Raf.) Sarg. by near infrared (NIR) spectroscopy
- Cellular distribution of coniferin in differentiating xylem of Chamaecyparis obtusa as revealed by Raman microscopy
- Hydrolysis of cellulose and wood powder treated with DMDHEU by a hydrolase enzyme complex, Fenton's reagent, and in a liquid culture of Trametes versicolor
- Effects of timing and intensity of thinning on wood structure and chemistry in Norway spruce
- Effects of calcium-based materials and iron impurities on wood degradation by the brown rot fungus Serpula lacrymans
- Lignin and carbohydrate variation with earlywood, latewood, and compression wood content of bent and straight ramets of a radiata pine clone
- Tensile stress relaxation of wood impregnated with different ACQ formulations at various temperatures
- Estimate of resistance-curve in wood through the double cantilever beam test
- Moisture-induced stresses and distortions in spruce cross-laminates and composite laminates
- Short Note
- Recovery of cellulose and xylan liquefied in ionic liquids by precipitation in anti-solvents
- Meetings
- Meetings
