Hydrolysis of cellulose and wood powder treated with DMDHEU by a hydrolase enzyme complex, Fenton's reagent, and in a liquid culture of Trametes versicolor
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Pradeep Verma
Abstract
Cellulose was treated with various concentrations of 1,3-dimethylol-4,5-dihydroxyethylene urea (DMDHEU) to obtain different DMDHEU contents in the cellulose preparation. The release of sugars due to hydrolysis by a cellulase enzyme complex clearly decreased with increasing DMDHEU content. When the cellulose preparation with the highest DMDHEU content was subjected to pretreatment with Fenton's reagent (Fe2+, H2O2), the sugar release increased 4.5-fold. Incubation of modified cellulose with only H2O2 did not increase sugar release by cellulase. Compared to cellulose, the sugar release from milled wood powder induced by cellulase was very low but still decreased due to treatment with DMDHEU. Sole incubation of wood powder with Fenton's reagent did barely cause more sugar release compared to the storage of wood powder in buffer, but when the pretreated wood was incubated with cellulase the sugar release was higher than without pretreatment. Release of sugar from untreated cellulose in liquid cultures of the white-rot fungus Trametes versicolor was detected after 7 days and increased linearly with incubation time. Sugar release from DMDHEU-treated cellulose was much lower and was not detectable before 21 days of incubation. Total protein content in the cultures was low, but was slightly higher in the cultures of untreated cellulose. Laccase activity was not different in the presence of untreated and DMDHEU-treated cellulose after 7 and 14 days, but increased with increasing DMDHEU content after 21 and 28 days.
©2010 by Walter de Gruyter Berlin New York
Articles in the same Issue
- Original Papers
- Initiating ECF bleaching sequences of eucalyptus kraft pulps with Z/D and Z/E stages
- Characterization of black liquors from kraft pulping of first-thinning Scots pine (Pinus sylvestris L.)
- Analysis of products from the oxidation of technical lignins by oxygen and H3PMo12O40 in water and aqueous methanol by size-exclusion chromatography
- Oxidative polymerisation of models for phenolic lignin end-groups by laccase
- Determination of anionic groups in wood by time-of-flight secondary ion mass spectrometry and laser ablation-inductively coupled plasma-mass spectrometry
- Effects of sample preparation on NIR spectroscopic estimation of chemical properties of Eucalyptus urophylla S.T. Blake wood
- Detection of wet-pockets on the surface of Tsuga heterophylla (Raf.) Sarg. by near infrared (NIR) spectroscopy
- Cellular distribution of coniferin in differentiating xylem of Chamaecyparis obtusa as revealed by Raman microscopy
- Hydrolysis of cellulose and wood powder treated with DMDHEU by a hydrolase enzyme complex, Fenton's reagent, and in a liquid culture of Trametes versicolor
- Effects of timing and intensity of thinning on wood structure and chemistry in Norway spruce
- Effects of calcium-based materials and iron impurities on wood degradation by the brown rot fungus Serpula lacrymans
- Lignin and carbohydrate variation with earlywood, latewood, and compression wood content of bent and straight ramets of a radiata pine clone
- Tensile stress relaxation of wood impregnated with different ACQ formulations at various temperatures
- Estimate of resistance-curve in wood through the double cantilever beam test
- Moisture-induced stresses and distortions in spruce cross-laminates and composite laminates
- Short Note
- Recovery of cellulose and xylan liquefied in ionic liquids by precipitation in anti-solvents
- Meetings
- Meetings
Articles in the same Issue
- Original Papers
- Initiating ECF bleaching sequences of eucalyptus kraft pulps with Z/D and Z/E stages
- Characterization of black liquors from kraft pulping of first-thinning Scots pine (Pinus sylvestris L.)
- Analysis of products from the oxidation of technical lignins by oxygen and H3PMo12O40 in water and aqueous methanol by size-exclusion chromatography
- Oxidative polymerisation of models for phenolic lignin end-groups by laccase
- Determination of anionic groups in wood by time-of-flight secondary ion mass spectrometry and laser ablation-inductively coupled plasma-mass spectrometry
- Effects of sample preparation on NIR spectroscopic estimation of chemical properties of Eucalyptus urophylla S.T. Blake wood
- Detection of wet-pockets on the surface of Tsuga heterophylla (Raf.) Sarg. by near infrared (NIR) spectroscopy
- Cellular distribution of coniferin in differentiating xylem of Chamaecyparis obtusa as revealed by Raman microscopy
- Hydrolysis of cellulose and wood powder treated with DMDHEU by a hydrolase enzyme complex, Fenton's reagent, and in a liquid culture of Trametes versicolor
- Effects of timing and intensity of thinning on wood structure and chemistry in Norway spruce
- Effects of calcium-based materials and iron impurities on wood degradation by the brown rot fungus Serpula lacrymans
- Lignin and carbohydrate variation with earlywood, latewood, and compression wood content of bent and straight ramets of a radiata pine clone
- Tensile stress relaxation of wood impregnated with different ACQ formulations at various temperatures
- Estimate of resistance-curve in wood through the double cantilever beam test
- Moisture-induced stresses and distortions in spruce cross-laminates and composite laminates
- Short Note
- Recovery of cellulose and xylan liquefied in ionic liquids by precipitation in anti-solvents
- Meetings
- Meetings
