Lignification of ray parenchyma cells in the xylem of Pinus densiflora. Part II: Microchemical analysis by laser microdissection and thioacidolysis
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Peiming Zheng
Abstract
The lignification of ray parenchyma cells in the sapwood (sW) and heartwood (hW) of Pinus densiflora was investigated by thioacidolysis and the subsequent Raney nickel desulfuration. The samples rich and less rich in ray parenchyma cells were prepared by laser microdissection (LMD). The whole sections burned randomly by the laser served as the controls. Guaiacyl (G) monomers were detected in all the samples, and p-hydroxyphenyl (H) monomers were detectable only in trace amounts, while syringyl (S) units were absent, as expected in softwood. In sW samples rich in ray parenchyma cells, the yields of G monomers are significantly lower than in the other samples. The various types of G-G and one G-H dimers were detected, and the β-1′, β-5′, and 5-5′ dimers were dominant. The relative distributions of lignin interunit linkages were very similar in all the samples regardless of the abundance of the ray parenchyma cells in the sW or hW tissues.
Acknowledgments
This work was supported by the Japan Society for the Promotion of Science (Nos. 21228004, 25252032, and 25114508). We thank Hiroyuki Yamamoto (Graduate School of Bioagricultural Science, Nagoya University) for the support with sample preparation and Ikuyo Mizuguchi (Graduate School of Medicine, Nagoya University) for the technical support with the LMD.
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©2014 by De Gruyter
Artikel in diesem Heft
- Frontmatter
- Original Articles
- Influence of cellulose supramolecular structure on strength properties of chemical pulp
- Morphological, mechanical, and optical properties of cypress papers
- Determination of ion exchange constants for pairs of metal ions to lignocellulosic materials by column chromatography
- Combustion behavior of oak wood (Quercus mongolica L.) modified by 1,3-dimethylol-4,5-dihydroxyethyleneurea (DMDHEU)
- Synthesis and antifungal activity of camphoric acid-based acylhydrazone compounds
- Lignification of ray parenchyma cells in the xylem of Pinus densiflora. Part I: Microscopic investigation by POM, UV microscopy, and TOF-SIMS
- Lignification of ray parenchyma cells in the xylem of Pinus densiflora. Part II: Microchemical analysis by laser microdissection and thioacidolysis
- Distribution of lignin in vascular bundles of coconut wood (Cocos nucifera) by cellular UV-spectroscopy and relationship between lignification and tensile strength in single vascular bundles
- Compression properties of vascular boundles and parenchyma of rattan (Plectocomia assamica Griff)
- Properties of wood-plastic composites (WPCs) reinforced with extracted and delignified wood flour
- Anisotropic elastic properties of common ash (Fraxinus excelsior L.)
- Destructive and non-destructive evaluation of seven hardwoods and analysis of data correlation
- Experimental validation of green wood peeling assisted by IR heating – some considerations of the analytical system design
- The influence of felling season and log-soaking temperature on the wetting and phenol formaldehyde adhesive bonding characteristics of birch veneer
- Comparative evaluation of various lignin determination methods on hemicellulose-rich fractions of spruce and birch obtained by pressurized hot-water extraction (PHWE) and subsequent ultrafiltration (UF)
- Conversion between basic density and apparent density at any moisture content in Eucalyptus grandis
- Postpeak residual capacity of nailed connections of a shear wall
- Obituary
- Memorial on Horst H. Nimz (1930–2013)
Artikel in diesem Heft
- Frontmatter
- Original Articles
- Influence of cellulose supramolecular structure on strength properties of chemical pulp
- Morphological, mechanical, and optical properties of cypress papers
- Determination of ion exchange constants for pairs of metal ions to lignocellulosic materials by column chromatography
- Combustion behavior of oak wood (Quercus mongolica L.) modified by 1,3-dimethylol-4,5-dihydroxyethyleneurea (DMDHEU)
- Synthesis and antifungal activity of camphoric acid-based acylhydrazone compounds
- Lignification of ray parenchyma cells in the xylem of Pinus densiflora. Part I: Microscopic investigation by POM, UV microscopy, and TOF-SIMS
- Lignification of ray parenchyma cells in the xylem of Pinus densiflora. Part II: Microchemical analysis by laser microdissection and thioacidolysis
- Distribution of lignin in vascular bundles of coconut wood (Cocos nucifera) by cellular UV-spectroscopy and relationship between lignification and tensile strength in single vascular bundles
- Compression properties of vascular boundles and parenchyma of rattan (Plectocomia assamica Griff)
- Properties of wood-plastic composites (WPCs) reinforced with extracted and delignified wood flour
- Anisotropic elastic properties of common ash (Fraxinus excelsior L.)
- Destructive and non-destructive evaluation of seven hardwoods and analysis of data correlation
- Experimental validation of green wood peeling assisted by IR heating – some considerations of the analytical system design
- The influence of felling season and log-soaking temperature on the wetting and phenol formaldehyde adhesive bonding characteristics of birch veneer
- Comparative evaluation of various lignin determination methods on hemicellulose-rich fractions of spruce and birch obtained by pressurized hot-water extraction (PHWE) and subsequent ultrafiltration (UF)
- Conversion between basic density and apparent density at any moisture content in Eucalyptus grandis
- Postpeak residual capacity of nailed connections of a shear wall
- Obituary
- Memorial on Horst H. Nimz (1930–2013)
