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Simultaneous measurement of 25(OH)-vitamin D and 24,25(OH)2-vitamin D to define cut-offs for CYP24A1 mutation and vitamin D deficiency in a population of 1200 young subjects

  • Etienne Cavalier EMAIL logo , Loreen Huyghebaert , Olivier Rousselle , Anne-Catherine Bekaert , Stéphanie Kovacs , Laura Vranken , Stéphanie Peeters , Caroline Le Goff and Aurélie Ladang
Published/Copyright: December 5, 2019
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Clinical Chemistry and Laboratory Medicine (CCLM)
From the journal Clinical Chemistry and Laboratory Medicine (CCLM) Volume 58 Issue 2

Abstract

Background

Simultaneous measurement of 25(OH)D and 24,25(OH)2D is a new tool for predicting vitamin D deficiency and allows evaluating CYP24A1 lack of function. Interpretation of 24,25(OH)2D should be performed according to 25(OH)D levels and a ratio, called the vitamin D metabolite ratio (VMR) has been proposed for such a purpose. Unfortunately, the VMR can be expressed in different ways and cannot be used if 24,25(OH)2D concentrations are undetectable. Here, we propose evaluating the enzyme activity taking into consideration the probability that a normal population presents undetectable 24,25(OH)2D concentrations according to 25(OH)D levels. We thus retrospectively measured 25(OH)D and 24,25(OH)2D in a population of 1200 young subjects to evaluate the 25(OH)D threshold above which the enzyme was induced.

Methods

Serum samples from 1200 infants, children, adolescent and young adults were used to simultaneously quantify 25(OH)D and 24,25(OH)2D by LCMS/MS.

Results

Median (interquartile range [IQR]) levels were 20.6 (14.4–27.2) ng/mL for 25(OH)D. 172 subjects (14.3%) presented 24,25(OH)2D values below the LOQ. When 25(OH)D values were <11 ng/mL, 63.1% of subjects presented undetectable 24,25(OH)2D concentrations. Percentage decreased with increasing 25(OH)D values to become 19.7% for 25(OH)D comprised between 12 and 15 ng/mL, 5.1% for 25(OH)D between 16 and 20 and 0.7% for 25(OH)D >21 ng/mL.

Conclusions

We suggest using a statistical approach to evaluate CYP24A1 function according to 25(OH)D concentrations. Our results also show that vitamin D deficiency, as defined biochemically, could be around 20 ng/mL in infants, children, adolescent and young adults and that vitamin D deficiency could be evaluated on a more individual basis.

Keywords: 24.25(OH)2-vitamin D; 25(OH)-vitamin D; CYP24A1; vitamin D; vitamin D metabolite ratio (VMR)
Corresponding author: Pr. Etienne Cavalier, Department of Clinical Chemistry, University of Liège, CHU de Liège, Domaine du Sart-Tilman, 4000 Liège, Belgium, Phone: +32 4 3667692, Fax: +32 4 3667691
aCaroline Le Goff and Aurélie Ladang contributed equally to this work.

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: None declared.

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

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Received: 2019-09-24
Accepted: 2019-11-06
Published Online: 2019-12-05
Published in Print: 2020-01-28

©2019 Walter de Gruyter GmbH, Berlin/Boston

Articles in the same Issue

  1. Frontmatter
  2. Editorial
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  4. Reviews
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https://doi.org/10.1515/cclm-2019-0996
Keywords for this article
24.25(OH)2-vitamin D; 25(OH)-vitamin D; CYP24A1; vitamin D; vitamin D metabolite ratio (VMR)

Articles in the same Issue

  1. Frontmatter
  2. Editorial
  3. Towards a personalized assessment of vitamin D status
  4. Reviews
  5. Circulating tumor DNA and their added value in molecular oncology
  6. Telomere length determinants in childhood
  7. Opinion Papers
  8. Serum or plasma? An old question looking for new answers
  9. Evaluating sample stability in the clinical laboratory with the help of linear and non-linear regression analysis
  10. General Clinical Chemistry and Laboratory Medicine
  11. Simultaneous measurement of 25(OH)-vitamin D and 24,25(OH)2-vitamin D to define cut-offs for CYP24A1 mutation and vitamin D deficiency in a population of 1200 young subjects
  12. How well do Croatian laboratories adhere to national recommendations for laboratory diagnostics of chronic kidney disease (CKD)?
  13. Underfilling of vacuum blood collection tubes leads to increased lactate dehydrogenase activity in serum and heparin plasma samples
  14. Calcium state estimation by total calcium: the evidence to end the never-ending story
  15. The use of faecal immunochemical testing in the decision-making process for the endoscopic investigation of iron deficiency anaemia
  16. Measurement uncertainty of β-lactam antibiotics results: estimation and clinical impact on therapeutic drug monitoring
  17. Practical approach to method verification in plasma and validation in cerebrospinal fluid under accreditation using a flexible scope in molecular virology: setting up the HIV, HBV and HCV Aptima™ Quant Dx assays
  18. Plasma neurofilament light chain is associated with mortality after spontaneous intracerebral hemorrhage
  19. Comparison of the diagnostic performance of two automated urine sediment analyzers with manual phase-contrast microscopy
  20. Development and validation of LC-MS/MS methods to measure tobramycin and lincomycin in plasma, microdialysis fluid and urine: application to a pilot pharmacokinetic research study
  21. Reference Values and Biological Variations
  22. Cord blood S100B: reference ranges and interest for early identification of newborns with brain injury
  23. Hematology and Coagulation
  24. Validation and standardization of the ETP-based activated protein C resistance test for the clinical investigation of steroid contraceptives in women: an unmet clinical and regulatory need
  25. Cancer Diagnostics
  26. Next-generation sequencing for tumor mutation quantification using liquid biopsies
  27. Cardiovascular Diseases
  28. Evolution of the slopes of ST2 and galectin-3 during marathon and ultratrail running compared to a control group
  29. Letters to the Editor
  30. Biological variation of two serum markers for preeclampsia prediction
  31. Daily monitoring of a control material with a concentration near the limit of detection improves the measurement accuracy of highly sensitive troponin assays
  32. Are patients adequately informed about procedures for 24-h urine collection?
  33. Interferences in free thyroxine concentration using the Roche analytical platform: improvement of the third generation?
  34. Procedures for the diagnosis of macro-follicle stimulating hormone (FSH) in a patient with high serum FSH concentrations
  35. False-positive result of immunochromatographic (IC) strip test for the diagnosis of α-thalassemia in samples with autoantibodies
  36. Sample dilution for soluble interleukin-2 receptor α measurement: comparison of two different matrices
  37. The growing problem of predatory publishing: a case report
  38. Cerebrospinal fluid lactate levels according to the site of puncture
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