IgA anticardiolipin and IgA anti-β2 glycoprotein I antibody positivity determined by fluorescence enzyme immunoassay in primary antiphospholipid syndrome
-
Elena Mattia
,
Amelia Ruffatti
Abstract
Background: Primary antiphospholipid syndrome (PAPS) is an autoimmune disease characterized by thrombosis and/or pregnancy morbidity as well as blood antiphospholipid (aPL) antibodies such as anticardiolipin (aCL), anti-β2 glycoprotein I (anti-β2GPI) antibodies of the IgG/IgM isotype and lupus anticoagulant (LA). The clinical significance of aCL and anti-β2GPI antibodies of the IgA isotype in PAPS is still a controversial issue.
Methods: Sera and plasma were collected from 84 PAPS patients (54 with thrombosis and/or pregnancy morbidity and 30 with pregnancy morbidity alone), 66 seronegative patients (subjects with clinical manifestations of PAPS although with negative results on conventional antiphospholipid antibody testing), and 78 healthy blood donors. IgA aCL and IgA anti-β2GPI were determined using fluorescence enzyme immunoassay (FEIA), (EliATM, Phadia AB, Uppsala, Sweden). For comparison purposes, the sera were also tested for IgG/IgM aCL/anti-β2GPI antibodies using the same immunoassay method. LA was assayed following internationally accepted guidelines.
Results: Present respectively in 19% and 50% of the PAPS patients studied, IgA aCL and IgA anti-β2GPI antibody frequencies were both statistically significant (p=0.001 and p<0.001, respectively). The mean titers of both IgA aCL and IgA anti-β2GPI antibodies were higher in the thrombotic patients, but only the latter were significantly associated with thrombosis. Isolated IgA anti-β2GPI antibody positivity was significantly prevalent (p=0.04) in seven (10.6%) of the seronegative patients.
Conclusions: Positivity to IgA anti-β2GPI antibody detected using FEIA was found to be clinically relevant in PAPS patients. Moreover the prevalence of isolated IgA anti-β2GPI antibody positivity was significant in the seronegative patients.
Conflict of interest statement
Authors’ conflict of interest disclosure: The authors declare that there are no conflicts of interest regarding this article.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
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©2014 by De Gruyter
Artikel in diesem Heft
- Frontmatter
- Editorial
- Red blood cell distribution width (RDW) and human pathology. One size fits all
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- Lactase persistence genotyping: rapid detection of seven sequence variants in a single tube with melting curve analyses
- General Clinical Chemistry and Laboratory Medicine
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- Improving CardioCheck PA analytical performance: three-year study
- Serum copeptin level predicts a rapid decrease of overhydration after kidney transplantation
- Thyroid function and thyroid autoimmunity in apparently healthy pregnant and non-pregnant Mexican women
- Serum and follicular fluid fetuin-A in women undergoing in vitro fertilization
- Value of reelin for assessing hepatic fibrogenesis in a group of Egyptian HCV infected patients
- IgA anticardiolipin and IgA anti-β2 glycoprotein I antibody positivity determined by fluorescence enzyme immunoassay in primary antiphospholipid syndrome
- Reference Values and Biological Variations
- Serum glucose adjusted cut-off values for normal cerebrospinal fluid/serum glucose ratio: implications for clinical practice
- Age- and gender-specific brain natriuretic peptide (BNP) reference ranges in primary care
- Cancer Diagnostics
- Clinical utility of %p2PSA and prostate health index in the detection of prostate cancer
- Clinical prognostic value of CD4+CD25+FOXP3+regulatory T cells in peripheral blood of Barcelona Clinic Liver Cancer (BCLC) stage B hepatocellular carcinoma patients
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- Cardiovascular Diseases
- Ideal cardiovascular health behaviors and factors and high sensitivity C-reactive protein: the Kailuan cross-sectional study in Chinese
- Letters to the Editor
- Red cell distribution width predicts chronic thromboembolic pulmonary hypertension in patients with acute pulmonary embolism in a long-term follow-up
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- Congress Abstracts
- 57th National Congress of the Hungarian Society of Laboratory Medicine
Artikel in diesem Heft
- Frontmatter
- Editorial
- Red blood cell distribution width (RDW) and human pathology. One size fits all
- Reviews
- LC-MS candidate reference methods for the harmonisation of parathyroid hormone (PTH) measurement: a review of recent developments and future considerations
- C-reactive protein and migraine. Facts or speculations?
- Mini Review
- ABO blood group and von Willebrand factor: biological implications
- Genetics and Molecular Diagnostics
- Lactase persistence genotyping: rapid detection of seven sequence variants in a single tube with melting curve analyses
- General Clinical Chemistry and Laboratory Medicine
- The 3rd International Standard for serum IgE: international collaborative study to evaluate a candidate preparation
- Improving CardioCheck PA analytical performance: three-year study
- Serum copeptin level predicts a rapid decrease of overhydration after kidney transplantation
- Thyroid function and thyroid autoimmunity in apparently healthy pregnant and non-pregnant Mexican women
- Serum and follicular fluid fetuin-A in women undergoing in vitro fertilization
- Value of reelin for assessing hepatic fibrogenesis in a group of Egyptian HCV infected patients
- IgA anticardiolipin and IgA anti-β2 glycoprotein I antibody positivity determined by fluorescence enzyme immunoassay in primary antiphospholipid syndrome
- Reference Values and Biological Variations
- Serum glucose adjusted cut-off values for normal cerebrospinal fluid/serum glucose ratio: implications for clinical practice
- Age- and gender-specific brain natriuretic peptide (BNP) reference ranges in primary care
- Cancer Diagnostics
- Clinical utility of %p2PSA and prostate health index in the detection of prostate cancer
- Clinical prognostic value of CD4+CD25+FOXP3+regulatory T cells in peripheral blood of Barcelona Clinic Liver Cancer (BCLC) stage B hepatocellular carcinoma patients
- Increased expression of carbonic anhydrase IX in oral submucous fibrosis and oral squamous cell carcinoma
- Cardiovascular Diseases
- Ideal cardiovascular health behaviors and factors and high sensitivity C-reactive protein: the Kailuan cross-sectional study in Chinese
- Letters to the Editor
- Red cell distribution width predicts chronic thromboembolic pulmonary hypertension in patients with acute pulmonary embolism in a long-term follow-up
- Red blood cell distribution width is significantly associated with aging and gender
- Red blood cell distribution width: a potential prognostic index for liver disease?
- Serum folate and vitamin B12: does light really matter?
- Thalassemia diagnosis in a blood donor from a unique trimorphic red blood cell population observed in the recipient
- A preliminary study on serum proteomics in fibromyalgia syndrome
- First study of angiotensin converting enzyme in cystic fibrosis Tunisian patients
- FlashFISH™: a novel technique for rapid diagnosis of aneuploidies in dysmorphic neonates
- Determination of the amniotic fluid lecithin/sphingomyelin ratio using a pipette tip column with a cation-exchange resin and mass spectrometry
- Congress Abstracts
- 57th National Congress of the Hungarian Society of Laboratory Medicine
