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Distributions and ranges of values of blood and urinary biomarker of inflammation and oxidative stress in the workers engaged in office machine manufactures: evaluation of reference values

  • Tadashi Murase EMAIL logo , Hiroko Kitamura , Takeshi Kochi , Niina Terunuma , Shizuka Kurosaki , Kouichi Hata , Nobuaki Yanagi , Bungo Uchino , Kayo Kitahara , Yasuo Morimoto , Hiroshi Kasai , Toshio Sasaki , Akira Ogami and Toshiaki Higashi
Published/Copyright: August 3, 2012
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Clinical Chemistry and Laboratory Medicine (CCLM)
From the journal Clinical Chemistry and Laboratory Medicine (CCLM) Volume 51 Issue 2

Abstract

Background: Interleukins, interferons and oxidative DNA products are important biomarkers assessing the inflammations and tissue damages caused by toxic materials in the body. We tried to evaluate distributions, reference values and age related changes of blood levels of inflammatory cytokines, C-reactive protein (CRP), IgE and urine levels of 8-hydroxy-2′-deoxyguanosine (8-OHdG) among workers in a cohort study evaluating the health influences of toner particles.

Methods: A total of 1366 male workers under age 50 years (age 19–49 years; 718 exposed and 648 not exposed to toner particles) in a cross sectional study of 1614 (categorized as 809 exposed and 805 not exposed, age 19–59 years) workers in a photocopier company has been followed prospectively as the cohort. Blood levels of interleukin (IL)-4, IL-6, IL-8, interferon-γ (IFN-γ), CRP, IgE and urine 8-OHdG were measured annually for 5 years.

Results: Reference values of the biomarkers are; CRP: 0.01–0.63×10-2 g/L, IgE: 6–1480 IU/mL, IL-4: 2.6–76.1 pg/mL, IL-6: 0.4–4.9 pg/mL and 8-OHdG: 1.5–8.2 ng/mgCr. We could not evaluate reference values for IL-8 and IFN-γ because most of the values were below the sensitivity limits (2.0 pg/mL and 0.1 IU/mL, respectively). There were no differences of the biomarker levels between the toner exposed and the control workers. We observed a statistically significant age related decrease of serum IL-4 levels.

Conclusions: This is the first report assessing the distributions and reference values of inflammatory biomarker levels in a large scaled cohort. We observed age related changes of some of the biomarkers. We could not detect any differences of the studied biomarker values between the toner exposed and the control workers.

Keywords: biomarker; cytokine; inflammatory biomarker; oxidative stress; reference value
Corresponding author: Tadashi Murase, MD, Department of Hematology and Oncology, Tokai University Hachioji Hospital, Tokai University School of Medicine, 192-0032, Tokyo, Japan, Phone: +81 42 6391111, Fax: +81 42 6391112

The cost of the study was provided to the University of Occupational and Environmental Health, Japan (UOEH) by Fuji Xerox Co., Ltd. as expenses for contract research. This article is based on a presentation at the 8th International Symposium on Biological Monitoring in Occupational and Environmental Health (ISBM 2010), 6–8 September 2010.

Conflict of interest statement

Authors’ conflict of interest disclosure: The authors stated that there are no conflicts of interest regarding the publication of this article. Research funding played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

Research funding: None declared.

Employment or leadership: None declared.

Honorarium: None declared.

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Received: 2012-05-28
Accepted: 2012-06-14
Published Online: 2012-08-03
Published in Print: 2013-02-01

©2013 by Walter de Gruyter Berlin Boston

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https://doi.org/10.1515/cclm-2012-0330
Keywords for this article
biomarker; cytokine; inflammatory biomarker; oxidative stress; reference value

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  1. Letters to the Editor
  2. Performance evaluation of three different immunoassays for detection of antibodies to hepatitis B core
  3. Serum homocysteine concentrations in Chinese children with autism
  4. Interchangeability of venous and capillary HbA1c results is affected by oxidative stress
  5. Interference of hemoglobin (Hb) N-Baltimore on measurement of HbA1c using the HA-8160 HPLC method
  6. First human isolate of Mycobacterium madagascariense in the sputum of a patient with tracheobronchitis
  7. Protein S and protein C measurements should not be undertaken during vitamin K antagonist therapy
  8. α2-HS glycoprotein is an essential component of cryoglobulin associated with chronic hepatitis C
  9. An unusual interference in CK MB assay caused by a macro enzyme creatine phosphokinase (CK) type 2 in HIV-infected patients
  10. An automated technique for the measurement of the plasma glutathione reductase activity and determination of reference limits for a healthy population
  11. Is osteopontin stable in plasma and serum?
  12. Evidence-based approach to reducing perceived wasteful practices in laboratory medicine
  13. Masthead
  14. Masthead
  15. Editorials
  16. Testing volume is not synonymous of cost, value and efficacy in laboratory diagnostics
  17. Lessons from controversy: biomarkers evaluation
  18. Commercial immunoassays in biomarkers studies: researchers beware!1)
  19. Trials and tribulations in lupus anticoagulant testing
  20. Reviews
  21. Mass spectrometry: a revolution in clinical microbiology?
  22. Chronic Chagas disease: from basics to laboratory medicine
  23. General Clinical Chemistry and Laboratory Medicine
  24. Shop for quality or quantity? Volumes and costs in clinical laboratories
  25. Minor improvement of venous blood specimen collection practices in primary health care after a large-scale educational intervention
  26. Evaluation of high resolution gel β2-transferrin for detection of cerebrospinal fluid leak
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  28. Soluble urokinase plasminogen activator receptor (suPAR) in the assessment of inflammatory activity of rheumatoid arthritis patients in remission
  29. Bone mass density selectively correlates with serum markers of oxidative damage in post-menopausal women
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