Refrigeration is not necessary for measurement of uric acid in patients treated with rasburicase
-
Neal I. Lindeman
,
Stacy E.F. Melanson
Abstract
Background: Rasburicase, used for hyperuricemia of tumor lysis syndrome, retains activity at room temperature (RT) in in vitro studies. Cold-temperature handling is recommended for uric acid measurements in patients receiving rasburicase: collection in prechilled tubes, transportation on ice, and 4°C centrifugation. We performed a prospective study of these requirements.
Methods: A total of 65 pairs of blood samples were collected from 34 patients, 12–24 h after receiving rasburicase. The effect of temperature on uric acid concentration was tested on paired samples handled either at RT or when cold: centrifugation (18 sample pairs), collection tube (14 pairs), transportation (24 pairs), and nine pairs were retested after 1 h at RT.
Results: No significant temperature effect was seen on the uric acid measurements for any of the cold-handling steps: proportional, absolute biases were –1.4%, –0.06 mg/dL (centrifugation), –1.5%, +0.02 mg/dL (tube temperature), and –2.2%, –0.01 mg/dL (transportation). A 20% negative bias was seen in samples retested after 1 h at RT.
Conclusions: Cold handling (prechilled tubes, iced transportation, 4°C centrifugation) was equivalent to RT for immediate measurement. An additional 1 h delay at RT led to a 20% decrease in uric acid. The cold handling measures required by the manufacturer are not necessary for uric acid testing of patients receiving rasburicase treatment, if testing is performed without delay.
Vera Beluvasava, Kristina Lefteri, Dulce Buencamino, Marcia Niland.
Conflict of interest statement
Authors’ conflict of interest disclosure: The authors stated that there are no conflicts of interest regarding the publication of this article.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
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©2013 by Walter de Gruyter Berlin Boston
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- Review
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- Mini Reviews
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- Glucose meters – fit for clinical purpose
- Opinion Papers
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- Recommendations for clinical laboratory science reports regarding properties, units, and symbols: the NPU format1)
- “Good samples make good assays” – the problem of sourcing clinical samples for a standardization project
- Defining acceptable limits for the metrological traceability of specific measurands
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