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Cysteinyl leukotrienes in exhaled breath condensate of smoking asthmatics

  • Deniz Celik , Sibel Doruk EMAIL logo , Handan Inonu Koseoglu , Semsettin Sahin , Serhat Celikel and Unal Erkorkmaz
Published/Copyright: October 6, 2012
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Clinical Chemistry and Laboratory Medicine
From the journal Clinical Chemistry and Laboratory Medicine Volume 51 Issue 5

Abstract

Background: Cysteinyl leukotrienes (CysLTs) are among important inflammatory mediators in asthma pathogenesis. In this study, we aimed to determine leukotriene D4 (LTD4) and leukotriene E4 (LTE4) levels in exhaled breath condensate (EBC) in asthmatics and to evaluate the effect of smoking upon CysLTs levels and to speculate the importance of adding leukotriene receptor antagonists in smoking asthmatics.

Methods: A total of 88 participants were included in the study. Of them, 59 were asthmatics; 30 of the 59 asthmatics were smokers (Group I) and the others were non-smokers (Group II). As a control group (Group III), 29 healthy non-smokers were enrolled. EBC samples were collected (EcoScreen, Jaeger, Hoechberg, Germany) and pulmonary function tests (PFTs) were performed in each case, and an asthma control questionnaire (ACQ) was completed by the asthmatics. LTD4 and LTE4 levels in EBC samples were analyzed by using ELISA.

Results: LTD4 levels were found to be higher in Group I than other groups and similar in Group II and Group III. LTE4 levels were lower in Group III than other groups and similar in Group I and Group II. PFTs were different between Group I and Group III and there was a significant negative correlation between LTE4 levels and forced expiratory volume in one second and forced vital capacity ratio in Group I.

Conclusions: LTD4, but not LTE4, concentrations in EBC were higher in asthmatic smokers than asthmatic non-smokers. LTE4 concentrations in EBC were higher in asthmatics than healthy non-smokers. In smoking asthmatics usage of agents that block the effects of LTD4 can be beneficial; however, new clinical studies are required.

Keywords: asthma; cigarette; cysteinyl leukotrienes; exhaled breath condensate; leukotriene D4 (LTD4); leukotriene E4 (LTE4); smoking
Corresponding author: Sibel Doruk, MD, Faculty of Medicine, Department of Pulmonary Medicine, Gaziosmanpasa University, 60100 Tokat, Turkey Phone: +90-356-2129500 (1310), Fax: +90-356-2133179

Conflict of interest statement

Authors’ conflict of interest disclosure: The authors stated that there are no conflicts of interest regarding the publication of this article.

Research funding: None declared.

Employment or leadership: None declared.

Honorarium: None declared.

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Received: 2012-7-25
Accepted: 2012-9-7
Published Online: 2012-10-06
Published in Print: 2013-05-01

©2013 by Walter de Gruyter Berlin Boston

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https://doi.org/10.1515/cclm-2012-0479
Keywords for this article
asthma; cigarette; cysteinyl leukotrienes; exhaled breath condensate; leukotriene D4 (LTD4); leukotriene E4 (LTE4); smoking

Articles in the same Issue

  1. Masthead
  2. Masthead
  3. Editorial
  4. Progress towards standardization: an IFCC Scientific Division Perspective
  5. Review
  6. Quantitative Clinical Chemistry Proteomics (qCCP) using mass spectrometry: general characteristics and application
  7. Mini Reviews
  8. Analytical goals for the determination of HbA2
  9. Glucose meters – fit for clinical purpose
  10. Opinion Papers
  11. Metrological traceability – a concept for standardization in laboratory medicine
  12. Recommendations for clinical laboratory science reports regarding properties, units, and symbols: the NPU format1)
  13. “Good samples make good assays” – the problem of sourcing clinical samples for a standardization project
  14. Defining acceptable limits for the metrological traceability of specific measurands
  15. Original Articles
  16. A reference system for urinary albumin: current status
  17. Toward standardization of carbohydrate-deficient transferrin (CDT) measurements: III. Performance of native serum and serum spiked with disialotransferrin proves that harmonization of CDT assays is possible
  18. External Quality Assessment Scheme for reference laboratories – review of 8 years’ experience
  19. Utility of a panel of sera for the alignment of test results in the worldwide multicenter study on reference values
  20. Protocol and standard operating procedures for common use in a worldwide multicenter study on reference values
  21. Guidelines and Recommendations
  22. 2013 update on the worldwide standardization of the hemoglobin A1c measurement
  23. General Clinical Chemistry and Laboratory Medicine
  24. Evacuated blood-collection tubes for haematological tests – a quality evaluation prior to their intended use for specimen collection
  25. Refrigeration is not necessary for measurement of uric acid in patients treated with rasburicase
  26. Procalcitonin and mid-regional pro-adrenomedullin test combination in sepsis diagnosis
  27. Cysteinyl leukotrienes in exhaled breath condensate of smoking asthmatics
  28. Helicobacter pylori serology in autoimmune diseases – fact or fiction?
  29. Serum DNase I activity in systemic lupus erythematosus: correlation with immunoserological markers, the disease activity and organ involvement
  30. Antibodies against Nε-homocysteinylated proteins in patients on different methods of renal replacement therapy
  31. Reference Values and Biological Variations
  32. Reference values for urinary neutrophil gelatinase-associated lipocalin (NGAL) in pediatric age measured with a fully automated chemiluminescent platform
  33. High biological variation of serum hyaluronic acid and Hepascore, a biochemical marker model for the prediction of liver fibrosis
  34. Cardiovascular Diseases
  35. Circulating matrix Gla protein: a potential tool to identify minor carotid stenosis with calcification in a risk population
  36. Midregional pro-atrial natriuretic peptide in the general population/Insights from the Gutenberg Health Study
  37. Letters to the Editor
  38. Relationship between Helicobacter pylori infection and autoimmune disorders
  39. Rapid simultaneous genotyping of polymorphisms in ADH1B and ALDH2 using high resolution melting assay
  40. Comparison of biological specimens and DNA collection methods for PCR amplification and microarray analysis
  41. Transferrin/log(ferritin) ratio: a self-fulfilling prophecy when iron deficiency is defined by serum ferritin concentration
  42. Response to: Transferrin/log(ferritin) ratio: a self-fulfilling prophecy when iron deficiency is defined by serum ferritin concentration
  43. Determinants of homocysteine concentrations in mother and neonatal girl pairs
  44. Cysteine analog breaks cryoprecipitate associated with chronic hepatitis C
  45. Cut-off values of serum growth hormone (GH) in pharmacological stimulation tests (PhT) evaluated in short-statured children using a chemiluminescent immunometric assay (ICMA) calibrated with the International Recombinant Human GH Standard 98/574
  46. Normalized MEDx chart as a useful tool for evaluation of analytical quality achievements. A picture is worth a thousand words
  47. Harmonization of immunoassays to the all-procedure trimmed mean – proof of concept by use of data from the insulin standardization project
  48. Congress Abstracts
  49. Annual Assembly of the Swiss Society of Clinical Chemistry & International Congress of Porphyrins and Porphyrias & International Meeting of Porphyria Patients: Personalized Medicine and Rare Diseases
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