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Comparison of capillary electrophoregram among heterozygous Hb Hope, Hb Hope/α-thalassemia-1 SEA type deletion and Hb Hope/β0-thalassemia
-
Sakorn Pornprasert
,
Sitthichai Panyasai
Veröffentlicht/Copyright:
7. März 2012
Abstract
Background: Hemoglobin (Hb) A2 is artifactually elevated in cases of heterozygous Hb Hope when measured by capillary electrophoresis (CE). However, there is no report of HbA2 levels and capillary electrophoregrams for associations of heterozygote of Hb Hope with α-thalassemia nor β-thalassemia.
Methods: Levels of HbA0, HbA2 and Hb Hope in 16 heterozygous Hb Hope, 3 Hb Hope/α-thalassemia-1 SEA type deletion and 2 Hb Hope/β0-thalassemia were measured by CE. Electrophoregram and the levels of those were compared within these three groups.
Results: Artifactually elevated HbA2 levels (≥4%) were found in both groups of heterozygous Hb Hope and Hb Hope/α-thalassemia-1 SEA type deletion. Manual corrections were performed by adjusting baselines, and results showed that means of HbA2 in both groups decreased from 4.47% and 4.03% to 1.93% and 1.77%, respectively. The highest levels of HbA2 and Hb Hope were observed in samples with Hb Hope/β0-thalassemia. Moreover, HbA0 was not observed in these cases.
Conclusions: The elevation of HbA2 in patients with heterozygous Hb Hope and with Hb Hope/α-thalassemia-1 SEA type deletion measured by CE leads to incorrect β-thalassemia trait diagnosis. However, using CE electrophoregram together with levels of HbA0, HbA2 and Hb Hope would be a more accurate and precise method for diagnosis of Hb Hope/β0-thalassemia.
Keywords: β0-thalassemia; capillary electrophoresis; electrophoregram; hemoglobin A2; hemoglobin Hope
Received: 2011-10-13
Accepted: 2012-2-6
Published Online: 2012-3-7
Published in Print: 2012-9-1
©2012 by Walter de Gruyter Berlin Boston
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Schlagwörter für diesen Artikel
β0-thalassemia;
capillary electrophoresis;
electrophoregram;
hemoglobin A2;
hemoglobin Hope
Artikel in diesem Heft
- Masthead
- Masthead
- Editorials
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- Serum creatinine and the search for new biomarkers of acute kidney injury (AKI): the story continues
- Interference of new oral anticoagulants with frequently used coagulation tests
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- General Clinical Chemistry and Laboratory Medicine
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- Validation of a new generation POCT glucose device with emphasis on aspects important for glycemic control in the hospital care
- Evaluation of NGAL Test™, a fully-automated neutrophil gelatinase-associated lipocalin (NGAL) immunoassay on Beckman Coulter AU 5822
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