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Application of a modified precipitation method for the measurement of small dense LDL-cholesterol (sd-LDL-C) in a population in southern Brazil
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Luciana da S. Cavalcante
Veröffentlicht/Copyright:
3. März 2012
Abstract
Background: In the study reported herein, we used the precipitation method employing heparin-Mg2+, with slight modifications to avoid lipemia interference, to measure small dense-low density lipoprotein-cholesterol (sd-LDL-C) in Brazilian subjects with a high risk of developing cardiovascular diseases.
Methods: Lipemic samples were diluted with various solvents prior to precipitation with heparin-Mg2+. Validation assays were performed with ultracentrifugation (n=100) and the reproducibility of sd-LDL-C measured in diluted serum (n=50). The applicability of this modification was evaluated by measuring sd-LDL-C in 434 southern Brazilian normolipidemic, dyslipidemic and type 2 diabetes mellitus (T2DM) individuals.
Results: Lipemic serum diluted with 100 mmol/L phosphate buffer pH 8.5 was effective for the quantification of sd-LDL-C, which was correlated with non-diluted serum (r=0.961; p<0.0001) and with ultracentrifugation (r=0.705; p<0.0001). Ultracentrifugation sd-LDL-C was 0.08 mmol/L (CI 95%: –0.42–0.58 mmol/L) higher than the precipitation method (p>0.05). Subjects with dyslipidemias and T2DM had, respectively, 2.3 and 2.6-fold higher sd-LDL-C concentrations than normolipidemic individuals (p<0.05). The incidence of normolipidemic subjects with a high concentration of sd-LDL-C was only 2.2%. The sd-LDL-C was found to be enhanced by 8.3% every 10 years and young normolipidemic men had 24% higher sd-LDL-C than young women (p<0.05). Lipid-lowering therapy reduced sd-LDL-C by 26% (p<0.001).
Conclusions: In this paper we described a simple and inexpensive approach to improving the measurement of sd-LDL-C in high-triglycerides serum. Furthermore, we showed that southern Brazil dyslipidemic and T2DM individuals have increased sd-LDL-C concentrations.
Keywords: cardiovascular risk; dyslipidemias; small dense-low density lipoprotein (sd-LDL); sd-LDL precipitation method; type 2 diabetes mellitus
Received: 2011-10-30
Accepted: 2012-1-24
Published Online: 2012-3-3
Published in Print: 2012-9-1
©2012 by Walter de Gruyter Berlin Boston
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Schlagwörter für diesen Artikel
cardiovascular risk;
dyslipidemias;
small dense-low density lipoprotein (sd-LDL);
sd-LDL precipitation method;
type 2 diabetes mellitus
Artikel in diesem Heft
- Masthead
- Masthead
- Editorials
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- Serum creatinine and the search for new biomarkers of acute kidney injury (AKI): the story continues
- Interference of new oral anticoagulants with frequently used coagulation tests
- Reviews
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