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Improvement in the performance of external quality assessment in Korean HIV clinical laboratories using unrecalcified human plasma

  • Jin-Sook Wang , Mee-Kyung Kee , Byeong-Sun Choi , Chan-Wha Kim , Hyon-Suk Kim and Sung Soon Kim EMAIL logo
Published/Copyright: March 16, 2012
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Clinical Chemistry and Laboratory Medicine (CCLM)
From the journal Clinical Chemistry and Laboratory Medicine (CCLM) Volume 50 Issue 9

Abstract

Background: The external quality assessment schemes (EQAS) organizer provides a suitable program to monitor and improve the quality of human immunodeficiency virus (HIV) testing laboratories with EQAS panels prepared under various conditions. The aim of the current study was to investigate the effects of human plasma samples on the EQAS results of HIV obtained from hospital-based clinical laboratories.

Methods: From 2007 to 2009, HIV EQAS panels consisted of four to six samples that consisted of undiluted positive and negative samples and were provided to laboratories twice per year. Up until the first half EQAS in 2008, EQAS panel materials were obtained by converting acid citrate dextrose treated plasma to serum via chemical treatment with CaCl2. Beginning with the second EQAS in 2008, all materials were prepared without the defibrination process.

Results: Approximately 300 HIV clinical laboratories participated in this program. The overall performance of clinical laboratories was shown to be improved when using unrecalcified plasma panels compared with recalcified panels. Significant differences were observed in EIA analyses of plasma for both positive (p<0.001) and negative (p<0.001) samples between the recalcified and unrecalcified groups.

Conclusions: Our finding suggested that defibrination status of EQAS panels might affect the results of anti-HIV EQAS of Korean HIV testing laboratories.

Keywords: external quality assessment schemes (EQAS) panel; HIV EQAS; HIV testing laboratory
Corresponding author: Sung Soon Kim, PhD, Laboratory Director, Division of AIDS, Center for Immunology and Pathology, Korea National Institute of Health Osong Health Technology Adminstration Complex, 187 Osongsaengmyeong2(i)-ro, Gangoe-myeon, Cheongwon-gun, Chungcheongbukdo, 363-951, South Korea Phone: +82 43 719 8410, Fax: +82 43 719 8459
Received: 2011-12-21
Accepted: 2012-2-23
Published Online: 2012-3-16
Published in Print: 2012-9-1

©2012 by Walter de Gruyter Berlin Boston

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https://doi.org/10.1515/cclm-2011-0943
Keywords for this article
external quality assessment schemes (EQAS) panel; HIV EQAS; HIV testing laboratory

Articles in the same Issue

  1. Masthead
  2. Masthead
  3. Editorials
  4. Neutrophil gelatinase-associated lipocalin (NGAL): the laboratory perspective
  5. Neutrophil gelatinase-associated lipocalin (NGAL): the clinician’s perspective
  6. Serum creatinine and the search for new biomarkers of acute kidney injury (AKI): the story continues
  7. Interference of new oral anticoagulants with frequently used coagulation tests
  8. Reviews
  9. Neutrophil gelatinase-associated lipocalin (NGAL) as biomarker of acute kidney injury: a review of the laboratory characteristics and clinical evidences
  10. Neurophil gelatinase-associated lipocalin as a new biomarker in laboratory medicine
  11. Neutrophil gelatinase-associated lipocalin as a biomarker of cardiovascular disease: a systematic review
  12. Opinion Paper
  13. Collective opinion paper on findings of the 2011 convocation of experts on laboratory quality
  14. Guidelines and Recommendations
  15. Proposals for the mitigation of the environmental impact of clinical laboratories
  16. General Clinical Chemistry and Laboratory Medicine
  17. Improvement in the performance of external quality assessment in Korean HIV clinical laboratories using unrecalcified human plasma
  18. Validation of a new generation POCT glucose device with emphasis on aspects important for glycemic control in the hospital care
  19. Evaluation of NGAL Test™, a fully-automated neutrophil gelatinase-associated lipocalin (NGAL) immunoassay on Beckman Coulter AU 5822
  20. Variation of serum and urinary neutrophil gelatinase associated lipocalin (NGAL) after strenuous physical exercise
  21. Analytic and clinical validation of a standardized cystatin C particle enhanced turbidimetric assay (PETIA) to estimate glomerular filtration rate
  22. Compensating for the influence of total serum protein in the Schwartz formula
  23. Interference of the new oral anticoagulant dabigatran with frequently used coagulation tests
  24. Testing for lupus anticoagulants – fresh or frozen?
  25. Effect of coagulation factors on discrepancies in International Normalized Ratio results between instruments
  26. Mobile Laboratory Unit: a disruptor solution for hemostasis management during major surgery. Usage in the context of face transplantation
  27. Comparison of capillary electrophoregram among heterozygous Hb Hope, Hb Hope/α-thalassemia-1 SEA type deletion and Hb Hope/β0-thalassemia
  28. Aberrant lamellar body counts noted on the Beckman Coulter Unicel DxH 800
  29. Plasma betaine concentrations correlate with plasma cortisol but not with C-reactive protein in an elderly population
  30. Determinants of the essential one-carbon metabolism metabolites, homocysteine, S-adenosylmethionine, S-adenosylhomocysteine and folate, in cerebrospinal fluid
  31. Application of a modified precipitation method for the measurement of small dense LDL-cholesterol (sd-LDL-C) in a population in southern Brazil
  32. Cancer Diagnostics
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  34. Clinical and pathological features of B-cell non-Hodgkin lymphomas lacking the surface expression of immunoglobulin light chains
  35. Statistical learning confirms the diagnostic significance of the anemia panel in breast cancer
  36. Letters to the Editor
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  42. Phosphate inhibits calcium oxalate crystal growth and crystallization through reducing free calcium ions: a morphological analysis and calcium consumption assay
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  44. 4th Slovenian Congress of Clinical Chemistry with international participation and 20th International Symposium of the Slovenian Association for Clinical Chemistry and Croatian Society of Medical Biochemists
  45. Congress of Clinical Chemistry and Laboratory Medicine / 9th Annual Meeting of the German Society for Clinical Chemistry and Laboratory Medicine (DGKL)
  46. Erratum
  47. Erratum to: Plasma betaine concentrations correlate with plasma cortisol but not with C-reactive protein in an elderly population
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