Artikel
Lizenziert
Nicht lizenziert
Erfordert eine Authentifizierung
Increased expression of the oncogenic KLF6-SV1 transcript in human glioblastoma
-
Andrei Tchirkov
Veröffentlicht/Copyright:
15. Juni 2010
Abstract
Background: Gliomas constitute the vast majority of primary central nervous system tumors in adults. Glioblastoma multiforme (GBM) is the most aggressive form of these primary brain tumors. There is a need to define diagnostic and prognostic markers that may help to distinguish GBM from non-GBM tumors. The Krüppel-like factor 6 (KLF6) gene has recently emerged as a promising candidate. The goal of our study was to determine if there is a link between KLF6 splice variants expression and different grades of gliomas.
Methods: Fifty-three primary gliomas tumor samples were analyzed using quantitative real-time PCR for the total KLF6, wild-type and alternatively spliced (SV1) KLF6 mRNA.
Results: Compared to the non-GBM group, the GBM group had a 2.2-fold increase in the mean level of total KLF6 mRNA expression. GBM showed a 2.1-fold increase in the KLF6 splicing ratio. In addition, KLF6-SV1 mRNA expression levels were also 2.2-fold higher in the GBM group, suggesting that the increase in the KLF6 splicing ratio was due to increased expression of the KLF6-SV1 oncogenic splice variant.
Conclusions: Our study demonstrates that quantification of total and spliced forms of KLF6 may provide a new and useful supplementary molecular tool for grading glioma.
Clin Chem Lab Med 2010;48:1167–70.
Received: 2009-10-28
Accepted: 2010-2-15
Published Online: 2010-06-15
Published in Print: 2010-08-01
©2010 by Walter de Gruyter Berlin New York
Sie haben derzeit keinen Zugang zu diesem Inhalt.
Sie haben derzeit keinen Zugang zu diesem Inhalt.
Artikel in diesem Heft
- Editorials
- Errors in transfusion medicine are not only misidentifications of the recipient, but also pre-analytical and analytical errors
- Biochemical and imaging biomarkers: the search for the Holy Grail
- Reviews
- Molecular diagnosis of congenital adrenal hyperplasia due to 21-hydroxylase deficiency: an update of new CYP21A2 mutations
- Potential role of the lipoxygenase derived lipid mediators in atherosclerosis: leukotrienes, lipoxins and resolvins
- Minireview
- Errors in transfusion: causes and measures to avoid them
- Opinion Paper
- Improving the harmonisation of the International Normalized Ratio (INR): time to think outside the box?
- Genetics and Molecular Diagnostics
- Differential contribution of MTHFR C677T variant to the risk of diabetic nephropathy in Lebanese and Bahraini Arabs
- Development and evaluation of an automated hepatitis C virus NS5B sequence-based subtyping assay
- A novel mutant-enriched liquidchip technology for the qualitative detection of somatic mutations in KRAS gene from both serum and tissue samples
- General Clinical Chemistry and Laboratory Medicine
- Urine particle evaluation: a comparison between the UF-1000i and quantitative microscopy
- Assessment of the Nova StatSensor whole blood point-of-care creatinine analyzer for the measurement of kidney function in screening for chronic kidney disease
- Non-invasive fetal RHD genotyping in the first trimester of pregnancy
- Cord blood and maternal serum neopterin concentrations in patients with pre-eclampsia
- Comparison of cetylpyridinium chloride and cetyltrimethylammonium bromide extractive procedures for quantification and characterization of human urinary glycosaminoglycans
- Increased serum concentrations of visfatin and its production by different joint tissues in patients with osteoarthritis
- Human immunodeficiency virus-infection induces major changes in high-density lipoprotein particle size distribution and composition: the effect of antiretroviral treatment and disease severity
- A less sensitive detector does not necessarily result in a less sensitive method: fast quantification of 13 antiretroviral analytes in plasma with liquid chromatography coupled with tandem mass spectrometry
- Reference Values and Biological Variations
- Homocysteine in small-for-gestational age and appropriate-for-gestational age preterm neonates from mothers receiving folic acid supplementation
- Cancer Diagnostics
- Circulating procalcitonin in aseptic carcinoma patients: a specificity study with 18F-fluorodeoxyglucose positron-emission tomography/computed tomography as benchmark
- Increased expression of the oncogenic KLF6-SV1 transcript in human glioblastoma
- Thyroglobulin measurement in fine-needle aspirates of lymph nodes in patients with differentiated thyroid cancer: a simple definition of the threshold value, with emphasis on potential pitfalls of the method
- Infectious Diseses
- Evaluation of the BacT/ALERT® 3D system for the implementation of in-house quality control sterility testing at Canadian Blood Services
- Letters to the Editor
- Should the external quality assessment sample of antinuclear antibodies be of a typical monospecific pattern?
- Identification of reduced serum thrombopoietin concentrations in patients with chronic hepatitis C undergoing an interferon therapy
- Plasma glycogen phosphorylase BB is associated with pulmonary artery wedge pressure and left ventricle mass index in patients with hypertrophic cardiomyopathy
- Target cell formation leading to pseudoleukocytosis in patients with malignant disorders
- Controversies on quotient reporting to standardize laboratory results
- Abstracts
- Fifth Santorini Conference Biologie Prospective 2010
Schlagwörter für diesen Artikel
gliomas;
glioblastoma multiforme;
KLF6-SV1;
Krüppel-like factor (KLF)6
Artikel in diesem Heft
- Editorials
- Errors in transfusion medicine are not only misidentifications of the recipient, but also pre-analytical and analytical errors
- Biochemical and imaging biomarkers: the search for the Holy Grail
- Reviews
- Molecular diagnosis of congenital adrenal hyperplasia due to 21-hydroxylase deficiency: an update of new CYP21A2 mutations
- Potential role of the lipoxygenase derived lipid mediators in atherosclerosis: leukotrienes, lipoxins and resolvins
- Minireview
- Errors in transfusion: causes and measures to avoid them
- Opinion Paper
- Improving the harmonisation of the International Normalized Ratio (INR): time to think outside the box?
- Genetics and Molecular Diagnostics
- Differential contribution of MTHFR C677T variant to the risk of diabetic nephropathy in Lebanese and Bahraini Arabs
- Development and evaluation of an automated hepatitis C virus NS5B sequence-based subtyping assay
- A novel mutant-enriched liquidchip technology for the qualitative detection of somatic mutations in KRAS gene from both serum and tissue samples
- General Clinical Chemistry and Laboratory Medicine
- Urine particle evaluation: a comparison between the UF-1000i and quantitative microscopy
- Assessment of the Nova StatSensor whole blood point-of-care creatinine analyzer for the measurement of kidney function in screening for chronic kidney disease
- Non-invasive fetal RHD genotyping in the first trimester of pregnancy
- Cord blood and maternal serum neopterin concentrations in patients with pre-eclampsia
- Comparison of cetylpyridinium chloride and cetyltrimethylammonium bromide extractive procedures for quantification and characterization of human urinary glycosaminoglycans
- Increased serum concentrations of visfatin and its production by different joint tissues in patients with osteoarthritis
- Human immunodeficiency virus-infection induces major changes in high-density lipoprotein particle size distribution and composition: the effect of antiretroviral treatment and disease severity
- A less sensitive detector does not necessarily result in a less sensitive method: fast quantification of 13 antiretroviral analytes in plasma with liquid chromatography coupled with tandem mass spectrometry
- Reference Values and Biological Variations
- Homocysteine in small-for-gestational age and appropriate-for-gestational age preterm neonates from mothers receiving folic acid supplementation
- Cancer Diagnostics
- Circulating procalcitonin in aseptic carcinoma patients: a specificity study with 18F-fluorodeoxyglucose positron-emission tomography/computed tomography as benchmark
- Increased expression of the oncogenic KLF6-SV1 transcript in human glioblastoma
- Thyroglobulin measurement in fine-needle aspirates of lymph nodes in patients with differentiated thyroid cancer: a simple definition of the threshold value, with emphasis on potential pitfalls of the method
- Infectious Diseses
- Evaluation of the BacT/ALERT® 3D system for the implementation of in-house quality control sterility testing at Canadian Blood Services
- Letters to the Editor
- Should the external quality assessment sample of antinuclear antibodies be of a typical monospecific pattern?
- Identification of reduced serum thrombopoietin concentrations in patients with chronic hepatitis C undergoing an interferon therapy
- Plasma glycogen phosphorylase BB is associated with pulmonary artery wedge pressure and left ventricle mass index in patients with hypertrophic cardiomyopathy
- Target cell formation leading to pseudoleukocytosis in patients with malignant disorders
- Controversies on quotient reporting to standardize laboratory results
- Abstracts
- Fifth Santorini Conference Biologie Prospective 2010
