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Urine particle evaluation: a comparison between the UF-1000i and quantitative microscopy

  • Fabio Manoni , Agostino Tinello , Lucia Fornasiero , Paolo Hoffer , Valeria Temporin , Sara Valverde and Gianluca Gessoni
Published/Copyright: May 19, 2010
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Clinical Chemistry and Laboratory Medicine
From the journal Clinical Chemistry and Laboratory Medicine Volume 48 Issue 8

Abstract

Background: The study of urine particles plays a key role in the diagnosis of kidney diseases. In this study, the authors evaluated the correlation between the UF-1000i and quantitative manual microscopy.

Methods: A total of 214 untreated urine samples were studied using the Sysmex UF-1000i and compared with results obtained from quantitative manual microscopy using the Fuchs-Rosenthal counting chamber.

Results: Using Pearson statistics, we observed satisfactory correlation between the UF-1000i and quantitative microscopy: for red blood cells (RBCs) r was 0.98, for white blood cells (WBCs) r was 1.00, for epithelial cells (EC) r was 0.96, and for casts r was 0.69. Using linear regression statistics, we also observed satisfactory correlation between the UF-1000i and quantitative microscopy: for RBCs R2 was 0.95, for WBCs R2 was 0.99, for EC R2 was 0.92, and for casts R2 was 0.48.

Conclusions: In our experience, automated urine particle analysis performed using the Sysmex UF-1000i analyzer is sufficiently precise and improves the workflow in a routine laboratory. Precision was satisfactory and concordance with the reference method is good for RBC, WBC and EC; for casts microscopic observation is required for flagged samples to discriminate hyaline from pathologic casts.

Clin Chem Lab Med 2010;48:1107–11.

Keywords: automated system; UF-1000i; urine analysis
Corresponding author: Gianluca Gessoni, Director Laboratory Medicine Service, Ospedale Civile di Chioggia, Via Madonna Marina 500, Venice, Italy Phone: +39 041 5534400, Fax: +39 041 5534401, ;
Received: 2009-12-28
Accepted: 2010;-2-26
Published Online: 2010-05-19
Published in Print: 2010-08-01

©2010 by Walter de Gruyter Berlin New York

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https://doi.org/10.1515/CCLM.2010.233
Keywords for this article
automated system; UF-1000i; urine analysis

Articles in the same Issue

  1. Editorials
  2. Errors in transfusion medicine are not only misidentifications of the recipient, but also pre-analytical and analytical errors
  3. Biochemical and imaging biomarkers: the search for the Holy Grail
  4. Reviews
  5. Molecular diagnosis of congenital adrenal hyperplasia due to 21-hydroxylase deficiency: an update of new CYP21A2 mutations
  6. Potential role of the lipoxygenase derived lipid mediators in atherosclerosis: leukotrienes, lipoxins and resolvins
  7. Minireview
  8. Errors in transfusion: causes and measures to avoid them
  9. Opinion Paper
  10. Improving the harmonisation of the International Normalized Ratio (INR): time to think outside the box?
  11. Genetics and Molecular Diagnostics
  12. Differential contribution of MTHFR C677T variant to the risk of diabetic nephropathy in Lebanese and Bahraini Arabs
  13. Development and evaluation of an automated hepatitis C virus NS5B sequence-based subtyping assay
  14. A novel mutant-enriched liquidchip technology for the qualitative detection of somatic mutations in KRAS gene from both serum and tissue samples
  15. General Clinical Chemistry and Laboratory Medicine
  16. Urine particle evaluation: a comparison between the UF-1000i and quantitative microscopy
  17. Assessment of the Nova StatSensor whole blood point-of-care creatinine analyzer for the measurement of kidney function in screening for chronic kidney disease
  18. Non-invasive fetal RHD genotyping in the first trimester of pregnancy
  19. Cord blood and maternal serum neopterin concentrations in patients with pre-eclampsia
  20. Comparison of cetylpyridinium chloride and cetyltrimethylammonium bromide extractive procedures for quantification and characterization of human urinary glycosaminoglycans
  21. Increased serum concentrations of visfatin and its production by different joint tissues in patients with osteoarthritis
  22. Human immunodeficiency virus-infection induces major changes in high-density lipoprotein particle size distribution and composition: the effect of antiretroviral treatment and disease severity
  23. A less sensitive detector does not necessarily result in a less sensitive method: fast quantification of 13 antiretroviral analytes in plasma with liquid chromatography coupled with tandem mass spectrometry
  24. Reference Values and Biological Variations
  25. Homocysteine in small-for-gestational age and appropriate-for-gestational age preterm neonates from mothers receiving folic acid supplementation
  26. Cancer Diagnostics
  27. Circulating procalcitonin in aseptic carcinoma patients: a specificity study with 18F-fluorodeoxyglucose positron-emission tomography/computed tomography as benchmark
  28. Increased expression of the oncogenic KLF6-SV1 transcript in human glioblastoma
  29. Thyroglobulin measurement in fine-needle aspirates of lymph nodes in patients with differentiated thyroid cancer: a simple definition of the threshold value, with emphasis on potential pitfalls of the method
  30. Infectious Diseses
  31. Evaluation of the BacT/ALERT® 3D system for the implementation of in-house quality control sterility testing at Canadian Blood Services
  32. Letters to the Editor
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  34. Identification of reduced serum thrombopoietin concentrations in patients with chronic hepatitis C undergoing an interferon therapy
  35. Plasma glycogen phosphorylase BB is associated with pulmonary artery wedge pressure and left ventricle mass index in patients with hypertrophic cardiomyopathy
  36. Target cell formation leading to pseudoleukocytosis in patients with malignant disorders
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  38. Abstracts
  39. Fifth Santorini Conference Biologie Prospective 2010
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