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Differential contribution of MTHFR C677T variant to the risk of diabetic nephropathy in Lebanese and Bahraini Arabs

  • Rita Nemr , Rabha A. Salman , Lamees H. Jawad , Eman A. Juma , Sose H. Keleshian and Wassim Y. Almawi
Published/Copyright: June 7, 2010
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Clinical Chemistry and Laboratory Medicine
From the journal Clinical Chemistry and Laboratory Medicine Volume 48 Issue 8

Abstract

Background: Methylenetetrahydrofolate reductase (MTHFR) gene variants and hyperhomocysteinemia have been implicated in the pathogenesis of diabetic nephropathy (DN) in various ethnic groups. We investigated the association of C677T and A1298C MTHFR gene variants and altered homocysteine concentrations in Lebanese and Bahraini type 2 diabetes (T2DM) DN patients.

Methods: Bahraini subjects comprised 224 DN patients and 328 T2DM patients with normal urine albumin [diabetes without nephropathy (DWN)]. Lebanese subjects comprised 252 DN and 309 DWN patients. C677T and A1298C genotypes were determined by PCR-restriction fragment length polymorphism (RFLP) analysis, and homocysteine was measured by ELISA.

Results:A1298C allele and genotype distribution were comparable between DN and DWN patients in both communities. However, there was enrichment of the 677T allele, together with C/T and T/T genotypes in Lebanese but not Bahraini DN patients, thereby conferring DN susceptibility [odds ratio (OR) (95% CI)=2.43 (1.89–3.11) and OR (95% CI)=1.15 (0.83–1.61), respectively; heterogeneity Q=12.53, p=0.0004)].

Conclusions: The contribution of C677T single nucleotide polymorphism to increased risk of DN (presumably by increasing homocysteine concentrations) must be evaluated in the context of the ethnic background.

Clin Chem Lab Med 2010;48:1091–4.

Keywords: diabetic nephropathy; homocysteine; methy-lenetetrahydrofolate reductase; type 2 diabetes
Corresponding author: Wassim Y. Almawi, PhD, Department of Medical Biochemistry, College of Medicine and Medical Sciences, Arabian Gulf University, PO Box 22979, Manama, Bahrain Phone: +973-39717118, Fax: +973-17271090,
Received: 2009-9-17
Accepted: 2010-2-8
Published Online: 2010-06-07
Published in Print: 2010-08-01

©2010 by Walter de Gruyter Berlin New York

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https://doi.org/10.1515/CCLM.2010.228
Keywords for this article
diabetic nephropathy; homocysteine; methy-lenetetrahydrofolate reductase; type 2 diabetes

Articles in the same Issue

  1. Editorials
  2. Errors in transfusion medicine are not only misidentifications of the recipient, but also pre-analytical and analytical errors
  3. Biochemical and imaging biomarkers: the search for the Holy Grail
  4. Reviews
  5. Molecular diagnosis of congenital adrenal hyperplasia due to 21-hydroxylase deficiency: an update of new CYP21A2 mutations
  6. Potential role of the lipoxygenase derived lipid mediators in atherosclerosis: leukotrienes, lipoxins and resolvins
  7. Minireview
  8. Errors in transfusion: causes and measures to avoid them
  9. Opinion Paper
  10. Improving the harmonisation of the International Normalized Ratio (INR): time to think outside the box?
  11. Genetics and Molecular Diagnostics
  12. Differential contribution of MTHFR C677T variant to the risk of diabetic nephropathy in Lebanese and Bahraini Arabs
  13. Development and evaluation of an automated hepatitis C virus NS5B sequence-based subtyping assay
  14. A novel mutant-enriched liquidchip technology for the qualitative detection of somatic mutations in KRAS gene from both serum and tissue samples
  15. General Clinical Chemistry and Laboratory Medicine
  16. Urine particle evaluation: a comparison between the UF-1000i and quantitative microscopy
  17. Assessment of the Nova StatSensor whole blood point-of-care creatinine analyzer for the measurement of kidney function in screening for chronic kidney disease
  18. Non-invasive fetal RHD genotyping in the first trimester of pregnancy
  19. Cord blood and maternal serum neopterin concentrations in patients with pre-eclampsia
  20. Comparison of cetylpyridinium chloride and cetyltrimethylammonium bromide extractive procedures for quantification and characterization of human urinary glycosaminoglycans
  21. Increased serum concentrations of visfatin and its production by different joint tissues in patients with osteoarthritis
  22. Human immunodeficiency virus-infection induces major changes in high-density lipoprotein particle size distribution and composition: the effect of antiretroviral treatment and disease severity
  23. A less sensitive detector does not necessarily result in a less sensitive method: fast quantification of 13 antiretroviral analytes in plasma with liquid chromatography coupled with tandem mass spectrometry
  24. Reference Values and Biological Variations
  25. Homocysteine in small-for-gestational age and appropriate-for-gestational age preterm neonates from mothers receiving folic acid supplementation
  26. Cancer Diagnostics
  27. Circulating procalcitonin in aseptic carcinoma patients: a specificity study with 18F-fluorodeoxyglucose positron-emission tomography/computed tomography as benchmark
  28. Increased expression of the oncogenic KLF6-SV1 transcript in human glioblastoma
  29. Thyroglobulin measurement in fine-needle aspirates of lymph nodes in patients with differentiated thyroid cancer: a simple definition of the threshold value, with emphasis on potential pitfalls of the method
  30. Infectious Diseses
  31. Evaluation of the BacT/ALERT® 3D system for the implementation of in-house quality control sterility testing at Canadian Blood Services
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  34. Identification of reduced serum thrombopoietin concentrations in patients with chronic hepatitis C undergoing an interferon therapy
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  39. Fifth Santorini Conference Biologie Prospective 2010
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