Article
Licensed
Unlicensed
Requires Authentication
Paternal exclusion: allele sharing in microsatellite testing
-
Venkanna Narkuti
Published/Copyright:
November 1, 2008
Abstract
Background: A paternity disagreement analyzed with 15 autosomal microsatellite markers indicated allele sharing between the mother, questioned child and the alleged father generating an inconclusive paternity result.
Design and methods: In total, 15 autosomal and 17 Y tandem repeat loci were analyzed using AmpFlSTR Identifiler, AmpFlSTR®Y-filer™ kits followed by six microsatellite markers on X chromosome in DNA extracted from peripheral blood samples of the mother, questioned child and alleged father.
Results: Microsatellite analysis examined with 15 autosomal short tandem repeats (STRs) indicated at least one allele sharing at 14 loci between the mother, questioned child and alleged father except for the TPOX locus where a paternal-child allele mismatch was observed. Y chromosome investigations using 17 repeat markers signified the case as non-paternity (exclusion). A complete match of the six X chromosome loci in the questioned child with the mother was observed.
Conclusions: Our investigations on inconclusive paternity due to atypical allele sharing in autosomal microsatellites were resolved with X- and Y-chromosome STR analyses confirming the case as non-paternity.
Clin Chem Lab Med 2008;46:1586–8.
Keywords: allele sharing; exclusion; mismatch; paternity testing; short tandem repeats (STRs); X-STR; Y-STR
Received: 2008-3-31
Accepted: 2008-7-20
Published Online: 2008-11-01
Published in Print: 2008-11-01
©2008 by Walter de Gruyter Berlin New York
You are currently not able to access this content.
You are currently not able to access this content.
Articles in the same Issue
- Editorial
- Biochemical markers in clinical cardiology: perspectives from present to future. 1st IFCC-Ortho Clinical Diagnostics Conference on Clinical Diagnosis and the Clinical Laboratory
- Highlight: Cardiovascular Biomarkers
- Reviews
- The clinical impact of the universal diagnosis of myocardial infarction
- Troponin revisited 2008: assay performance
- Standardization of troponin I measurements: an update
- Biochemistry of B-type natriuretic peptide – where are we now?
- Clinical relevance of non-cardiac determinants of natriuretic peptide levels
- What is the value of B-type natriuretic peptide testing for diagnosis, prognosis or monitoring of critically ill adult patients in intensive care?
- Natriuretic peptide testing in primary care patients
- Natriuretic peptide testing in emergency settings
- Minireviews
- Oxidative stress, free radicals and bone remodeling
- Plasma homocysteine and vascular disease in elderly patients with mental illness
- Opinion Papers
- Fate of abstracts presented at the 2002 IFCC meeting
- Transition from congress abstract to full paper: the case of a national Argentinean congress in clinical laboratory
- Genetics and Molecular Diagnostics
- Association of endothelin-1 gene polymorphisms with variant angina in Korean patients
- Single nucleotide polymorphisms in ABCG5 and ABCG8 genes in Chilean subjects with polygenic hypercholesterolemia and controls
- Paternal exclusion: allele sharing in microsatellite testing
- General Clinical Chemistry
- Rapid sample preparation and simultaneous quantitation of prostaglandins and lipoxygenase derived fatty acid metabolites by liquid chromatography-mass spectrometry from small sample volumes
- Plasma bilirubin correlations in non-obstructive cholestasis after partial hepatectomy
- Aggregation of lipoprotein and inflammatory parameters in families with a history of premature myocardial infarction: the Tallinn Myocardial Infarction Study
- Significant elevation of a Th2 cytokine, interleukin-10, in pelvic inflammatory disease
- Hemoglobin A1c determination in the A1C-Derived Average Glucose (ADAG) study
- The effect of the mode of delivery on maternal-neonatal interleukin-6, biogenic amine and their precursor amino acid concentrations
- A rapid liquid chromatography-tandem mass spectrometry analysis of whole blood sirolimus using turbulent flow technology for online extraction
- Validation and Outcome Studies
- Benefits of the iQ200 automated urine microscopy analyser in routine urinalysis
- Electrochemical detection of blood alcohol concentration using a disposable biosensor based on screen-printed electrode modified with Nafion and gold nanoparticles
- About the z-multiplier in total error calculations
- Letters to the Editor
- A hypothyroid patient with increased free thyroid hormones
- Evaluation of blood collection tubes specific for homocysteine measurement
- Influence of assay-dependent variability of serum insulin levels on insulin sensitivity indices
- Frequency and type of preanalytical errors in a laboratory medicine department in India
- Sample dilution causes a positive bias on C-reactive protein using VITROS reagents, diluents and instruments – clinical implications?
Keywords for this article
allele sharing;
exclusion;
mismatch;
paternity testing;
short tandem repeats (STRs);
X-STR;
Y-STR
Articles in the same Issue
- Editorial
- Biochemical markers in clinical cardiology: perspectives from present to future. 1st IFCC-Ortho Clinical Diagnostics Conference on Clinical Diagnosis and the Clinical Laboratory
- Highlight: Cardiovascular Biomarkers
- Reviews
- The clinical impact of the universal diagnosis of myocardial infarction
- Troponin revisited 2008: assay performance
- Standardization of troponin I measurements: an update
- Biochemistry of B-type natriuretic peptide – where are we now?
- Clinical relevance of non-cardiac determinants of natriuretic peptide levels
- What is the value of B-type natriuretic peptide testing for diagnosis, prognosis or monitoring of critically ill adult patients in intensive care?
- Natriuretic peptide testing in primary care patients
- Natriuretic peptide testing in emergency settings
- Minireviews
- Oxidative stress, free radicals and bone remodeling
- Plasma homocysteine and vascular disease in elderly patients with mental illness
- Opinion Papers
- Fate of abstracts presented at the 2002 IFCC meeting
- Transition from congress abstract to full paper: the case of a national Argentinean congress in clinical laboratory
- Genetics and Molecular Diagnostics
- Association of endothelin-1 gene polymorphisms with variant angina in Korean patients
- Single nucleotide polymorphisms in ABCG5 and ABCG8 genes in Chilean subjects with polygenic hypercholesterolemia and controls
- Paternal exclusion: allele sharing in microsatellite testing
- General Clinical Chemistry
- Rapid sample preparation and simultaneous quantitation of prostaglandins and lipoxygenase derived fatty acid metabolites by liquid chromatography-mass spectrometry from small sample volumes
- Plasma bilirubin correlations in non-obstructive cholestasis after partial hepatectomy
- Aggregation of lipoprotein and inflammatory parameters in families with a history of premature myocardial infarction: the Tallinn Myocardial Infarction Study
- Significant elevation of a Th2 cytokine, interleukin-10, in pelvic inflammatory disease
- Hemoglobin A1c determination in the A1C-Derived Average Glucose (ADAG) study
- The effect of the mode of delivery on maternal-neonatal interleukin-6, biogenic amine and their precursor amino acid concentrations
- A rapid liquid chromatography-tandem mass spectrometry analysis of whole blood sirolimus using turbulent flow technology for online extraction
- Validation and Outcome Studies
- Benefits of the iQ200 automated urine microscopy analyser in routine urinalysis
- Electrochemical detection of blood alcohol concentration using a disposable biosensor based on screen-printed electrode modified with Nafion and gold nanoparticles
- About the z-multiplier in total error calculations
- Letters to the Editor
- A hypothyroid patient with increased free thyroid hormones
- Evaluation of blood collection tubes specific for homocysteine measurement
- Influence of assay-dependent variability of serum insulin levels on insulin sensitivity indices
- Frequency and type of preanalytical errors in a laboratory medicine department in India
- Sample dilution causes a positive bias on C-reactive protein using VITROS reagents, diluents and instruments – clinical implications?
