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Electrochemical detection of blood alcohol concentration using a disposable biosensor based on screen-printed electrode modified with Nafion and gold nanoparticles
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Peng Luo
Published/Copyright:
November 1, 2008
Abstract
Background: Blood alcohol determination plays an important role in laboratory medicine and forensic medicine. Nowadays, many methods are being used for alcohol measurement, but these methods are time-consuming and complex to perform laborious sample pre-treatment. The disposable amperometric biosensor, due to its portability, low cost and potential for fabrication, should be readily applicable for blood alcohol determination.
Methods: The biosensor was fabricated by immobilizing alcohol dehydrogenase and nicotinamide adenine dinucleotide coated by Nafion combined with gold nanoparticles onto the surface of screen-printed electrode modified with Meldola's blue. Evaluations of biosensor performance were performed according to the relevant National Committee for Clinical Laboratory Standards standard.
Results: The biosensor response for serum alcohol presents good linearity, precision, stability, accuracy, and specificity.
Conclusions: The biosensor exhibits the capability of detecting blood alcohol concentration in the clinical laboratory and in forensic medicine, unnecessarily performing laborious sample pre-treatment.
Clin Chem Lab Med 2008;46:1641–7.
Keywords: blood alcohol concentration; disposable biosensor; electrochemical detection; gold nanoparticles; screen-printed electrode
Received: 2008-3-14
Accepted: 2008-7-8
Published Online: 2008-11-01
Published in Print: 2008-11-01
©2008 by Walter de Gruyter Berlin New York
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Keywords for this article
blood alcohol concentration;
disposable biosensor;
electrochemical detection;
gold nanoparticles;
screen-printed electrode
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