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IFCC Guideline for sampling, measuring and reporting ionized magnesium in plasma

  • International Federation of Clinical Chemistry and Laboratory Medicine (IFCC): IFCC Scientific Division Committee on Point of Care Testing: Mohammed C. Ben Rayana , Robert W. Burnett , Arthur K. Covington , Paul D'Orazio , Niels Fogh-Andersen , Ellis Jacobs , Wolf R. Külpmann , Katsuhiko Kuwa , Lasse Larsson , Andrzej Lewenstam , Anton H.J. Maas , Gerhard Mager , Jerzy W. Naskalski , Anthony O. Okorodudu , Christoph Ritter and Andrew St John
Published/Copyright: July 30, 2007
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Clinical Chemistry and Laboratory Medicine
From the journal Clinical Chemistry and Laboratory Medicine Volume 46 Issue 1

Abstract

Analyzers with ion-selective electrodes (ISEs) for ionized magnesium (iMg) should yield comparable and unbiased results for iMg. This IFCC guideline on sampling, measuring and reporting iMg in plasma provides a prerequisite to achieve this goal [in this document, “plasma” refers to circulating plasma and the forms in which it is sampled, namely the plasma phase of anticoagulated whole blood (or “blood”), plasma separated from blood cells, or serum].

The guideline recommends measuring and reporting ionized magnesium as a substance concentration relative to the substance concentration of magnesium in primary aqueous calibrants with magnesium, sodium, and calcium chloride of physiological ionic strength. The recommended name is “the concentration of ionized magnesium in plasma”. Based on this guideline, results will be approximately 3% higher than the true substance concentration and 4% lower than the true molality in plasma.

Calcium ions interfere with all current magnesium ion-selective electrodes (Mg-ISEs), and thus it is necessary to determine both ions simultaneously in each sample and correct the result for Ca2+ interference. Binding of Mg in plasma is pH-dependent. Therefore, pH should be measured simultaneously with iMg to allow adjustment of the result to pH 7.4.

The concentration of iMg in plasma may be physiologically and clinically more relevant than the concentration of total magnesium. Furthermore, blood-gas analyzers or instruments for point-of-care testing are able to measure plasma iMg using whole blood (with intact blood cells) as the sample, minimizing turn-around time compared to serum and plasma, which require removal of blood cells.

Clin Chem Lab Med 2008;46:21–6.

Keywords: erythrocyte effect; heparin; influence factors; interference; ionized calcium; ionized magnesium; ion-selective electrode; lipophilic anions; liquid junction potential; pH; protein; sampling; silicone; sodium
Corresponding author: Dr. Niels Fogh-Andersen, Department of Clinical Biochemistry, Herlev Hospital, Herlev Ringvej 75, 2730 Herlev, Denmark Phone: +45-4-4883313, Fax: +45-4-4883311,
Received: 2006-11-9
Published Online: 2007-07-30
Published in Print: 2008-01-01

©2008 by Walter de Gruyter Berlin New York

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https://doi.org/10.1515/CCLM.2008.001
Keywords for this article
erythrocyte effect; heparin; influence factors; interference; ionized calcium; ionized magnesium; ion-selective electrode; lipophilic anions; liquid junction potential; pH; protein; sampling; silicone; sodium

Articles in the same Issue

  1. Editorial
  2. Improving healthcare through advances in point-of-care technologies
  3. Review
  4. New creatinine sensor for point-of-care testing of creatinine meets the National Kidney Disease Education Program guidelines
  5. Original Papers
  6. Performance of a multi-profile critical care testing analyzer
  7. Comparative study of calculated and measured total carbon dioxide
  8. Letter to the Editor
  9. Performance of immunofluorometric point-of-care assays for free pregnancy-associated plasma protein A detection in whole blood samples
  10. Guidelines and Recommendations
  11. IFCC Guideline for sampling, measuring and reporting ionized magnesium in plasma
  12. Reviews
  13. Critical evaluation of 1H NMR metabonomics of serum as a methodology for disease risk assessment and diagnostics
  14. The entero-insular axis: implications for human metabolism
  15. Genetics and Molecular Diagnostics
  16. Functional polymorphisms in the promoter of the matrix metalloproteinase-9 (MMP-9) gene are not linked with significant plasma MMP-9 variations in healthy subjects
  17. Genetic profiling in healthy subjects from the Stanislas cohort based on 24 polymorphisms: effects on biological variables
  18. Relationship between methionine synthase, methionine synthase reductase genetic polymorphisms and deep vein thrombosis among South Indians
  19. Simultaneous genotyping of the three lactose tolerance-linked polymorphisms LCT –13907C>G, LCT –13910C>T and LCT –13915T>G with Pyrosequencing™ technology
  20. A new high-throughput screening method for the detection of chronic lymphatic leukemia and myelodysplastic syndrome
  21. General Clinical Chemistry and Laboratory Medicine
  22. Saliva analysis by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF/MS): from sample collection to data analysis
  23. Mechanism of sodium loss with muscle sodium deficiency in sodium supplemented and unsupplemented subjects during hypokinesia
  24. Increased levels of 8-hydroxydeoxyguanosine and its relationship with lipid peroxidation and antioxidant vitamins in lung cancer
  25. Lipid peroxidation in stroke patients
  26. Resistin is present in human breast milk and it correlates with maternal hormonal status and serum level of C-reactive protein
  27. Waist-to-hip ratio correlates with homocysteine levels in male patients with coronary artery disease
  28. Isoenzymes of N-acetyl-β-hexosaminidase in human pleomorphic adenoma and healthy salivary glands: a preliminary study
  29. The in vivo and in vitro effects of L-carnitine supplementation on the erythrocyte membrane acetylcholinesterase, Na+, K+-ATPase and Mg2+-ATPase activities in basketball players
  30. Validation and Outcome Studies
  31. Analytical evaluation of the Optium Xido blood glucose meter
  32. Letters to the Editor
  33. New biomarkers of myocardial injury and assessment of cardiac toxicity during preparative regimen and hematopoietic cell transplantation in acute leukemia
  34. Analysis of serum and urinary lysophospholipase D/autotaxin in nephrotic syndrome
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