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Pitfalls in measuring the endocannabinoid 2-arachidonoyl glycerol in biological samples
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Michael Vogeser
Published/Copyright:
August 9, 2007
Abstract
Background: The endocannabinoid 2-arachidonoyl glycerol (2-AG) undergoes spontaneous isomerization to biologically inactive 1-AG. This effect has not been adequately addressed in previous studies that reported 2-AG concentrations in biological samples.
Methods: Liquid chromatography tandem-mass spectrometry (LC-MS/MS) was used for 1-AG and 2-AG analyses.
Results: Identical collision-induced disintegration spectra were found for 1-AG and 2-AG. For specific detection of both compounds, which share a common mass transition, baseline chromatographic separation is mandatory, even when applying MS/MS technology with its generally high detection specificity. When using standard chromatographic conditions with the very short run times typically used in LC-MS/MS methods, co-elution of 2-AG with 1-AG, which is present in human serum, causes false 2-AG results.
Conclusions: Our data highlight that the analytical specificity of MS/MS can be limited by interference from isobaric isomers with identical disintegration patterns. The specificity of this technology must be carefully evaluated for each individual application.
Clin Chem Lab Med 2007;45:1023–5.
Keywords: 1-arachidonoyl glycerol (1-AG); 2-arachidonoyl glycerol (2-AG); endocannabinoids; interference; isomers; liquid chromatography tandem-mass spectrometry (LC-MS/MS)
Received: 2007-2-1
Accepted: 2007-5-3
Published Online: 2007-08-09
Published in Print: 2007-08-01
©2007 by Walter de Gruyter Berlin New York
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