Establishment of detailed reference values for luteinizing hormone, follicle stimulating hormone, estradiol, and progesterone during different phases of the menstrual cycle on the Abbott ARCHITECT® analyzer
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Reto Stricker
Abstract
During a normal menstrual cycle, serum levels of luteinizing hormone (LH), follicle-stimulating hormone (FSH), estradiol, and progesterone can vary widely between cycles for the same woman, as well as between different woman. Reliable reference values based on the local population are important for correct interpretation of laboratory results. The purpose of our study was to determine detailed reference values for these hormones throughout the menstrual cycle using the Abbott ARCHITECT system. From 20 volunteers (age 20–36years) with normal cycles and no use of oral contraceptives, samples were taken every day during their cycle. Volunteers received three vaginal ultrasound examinations (days 10 and 13, and 1 or 2days after ovulation) to measure follicular and corpus luteum development. Hormone levels were measured using the corresponding ARCHITECT assay and were synchronized to the LH peak. Median, and 5th and 95th percentile values were determined for each day of the cycle, as well as for early follicular (days −15 to −6), late follicular (days −5 to −1), LH peak (day 0), early luteal (+1 to +4), mid-luteal (days +5 to +9), and late luteal (days +10 to +14) phases of the cycle. Based on our data, we were able to establish detailed reference values for LH, FSH, estradiol, and progesterone, which should aid in the interpretation of results for these reproductive hormones in a variety of circumstances.
Clin Chem Lab Med 2006;44:883–7.
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©2006 by Walter de Gruyter Berlin New York
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Articles in the same Issue
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- Recommendation to treat continuous variable errors like attribute errors
- Polymorphisms associated with apolipoprotein B levels in Greek patients with familial hypercholesterolemia
- Lack of association between α2B-adrenergic receptor polymorphism and risk of restenosis following coronary angioplasty and stent implantation – preliminary report
- Prognostic value of homocysteinemia in patients with congestive heart failure
- Comparability of indices for insulin resistance and insulin secretion determined during oral glucose tolerance tests
- Evaluation of clinical markers of atherosclerosis in young and elderly Japanese adults
- Hypoadiponectinemia is associated with symptomatic atherosclerotic peripheral arterial disease
- Cardiac troponin T and amino-terminal pro-natriuretic peptide concentrations in fetuses in the second trimester and in healthy neonates
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- Falsely elevated troponin I attributed to inadequate centrifugation using the Access® immunoassay analyzer
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