Determination of the length of sedimentation reaction in blood using the TEST 1 system: comparison with the Sedimatic 100 method, turbidimetric fibrinogen levels, and the influence of M-proteins
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Nasser E. Ajubi
Abstract
Background: Determination of the length of sedimentation reaction in blood (LSRB) is frequently used in daily practice to assess disease intensity. Recently, a micro-sedimentation method was introduced (TEST 1™) that uses EDTA anti-coagulated blood samples. The aim of this study was to characterize this method by comparing it to a conventional Westergren method (Sedimatic 100). Furthermore, correlation between fibrinogen and the LSRB and the influence of M-proteins on the LSRB was investigated.
Methods: Unselected paired samples were used for comparison between the TEST 1 and Sedimatic 100 methods (n=733); fibrinogen was measured in EDTA samples (n=765) using a turbidimetric method. Furthermore, LSRB was measured in 29 EDTA samples in paired serum tubes from patients in whom an M-protein was detected.
Results: TEST 1 showed excellent correlation with the Sedimatic 100 method (y=1.00x; n=733; r=0.92, 95% CI 0.90–0.93; p<0.0001), and had no significant bias (0.15mm/h, 95% CI –0.48 to 0.75mm/h). Furthermore, TEST 1 LSRB showed satisfactory correlation with the fibrinogen content (y=3.13+0.06x; n=765; r=0.78, 95% CI 0.75–0.80; p<0.0001). In samples containing M-proteins, satisfactory correlation between the M-protein content and TEST 1 LSRB was found (y=0.69+0.22x; n=29; r=0.71, 95% CI 0.45–0.85; p<0.0001), while excellent correlation was found when only M-proteins of the IgM type were taken into account (y=–0.95+0.23x; n=9; r=0.93, 95% CI 0.71–0.99; p<0.0002).
Conclusions: The results confirm previous reports that TEST 1 is a reliable method to measure the LSRB, and shows for the first time the quantitative relationship between TEST 1 LSRB and M-proteins, particularly those of the IgM type.
Clin Chem Lab Med 2006;44:904–6.
References
1. International Council for Standardization in Hematology (Expert Panel on Blood Rheology): ICSH recommendations for measurement of erythrocyte sedimentation rate. J Clin Pathol 1993; 46:198–203.10.1136/jcp.46.3.198Search in Google Scholar
2. Sox HC, Liang MH. The erythrocyte sedimentation rate. Ann Intern Med 1986; 4:515–23.Search in Google Scholar
3. Johansson J, Sigurdsson T, Holberg L, Bergstrom R. Erythrocyte sedimentation rate as a tumor marker in human prostatic cancer. Cancer 1992; 6:1556–63.10.1002/1097-0142(19920915)70:6<1556::AID-CNCR2820700619>3.0.CO;2-ISearch in Google Scholar
4. Gillum RF, Mussolino ME, Makuc DM. Erythrocyte sedimentation rate and coronary heart disease: the NHANES I epidemiologic follow-up study. J Clin Epidmiol 1995; 3:353–61.10.1016/0895-4356(94)00156-KSearch in Google Scholar
5. Gabay C, Kushner I. Acute-phase proteins and other systematic responses to inflammation. N Engl J Med 1999; 340:186–9.10.1056/NEJM199902113400607Search in Google Scholar
6. Plebani M, De Toni S, Sanzari MC, Bernardi D, Stockreiter E. The TEST 1 automated system. A new method for measuring the erythrocyte sedimentation rate. Am J Clin Pathol 1998; 110:334–40.10.1093/ajcp/110.3.334Search in Google Scholar
7. Piva E, Sanzari MC, Servidio G, Plebani M. Length of sedimentation reaction in undiluted blood (erythrocyte sedimentation rate): variations with sex and age and reference limits. Clin Chem Lab Med 2001; 39:451–4.10.1515/CCLM.2001.071Search in Google Scholar
8. Piva E, Fassina P, Plebani M. Determination of the length of sedimentation reaction (erythrocyte sedimentation rate) in non-anticoagulated blood with the Microtest 1. Clin Chem Lab Med 2002; 40:713–7.10.1515/CCLM.2002.123Search in Google Scholar
9. Romero A, Muñoz M, Ramírez G. Length of sedimentation reaction in blood: a comparison of the Test 1 ESR system with the ICSH reference method and the Sedisystem 15. Clin Chem Lab Med 2003; 41:232–7.10.1515/CCLM.2003.037Search in Google Scholar
10. Bakker AJ, Gorgels JP, Draaisma J, Jongendijk M, Altena L, Hamersma A, et al. Simple method for correcting total protein in plasma for actual fibrinogen content. Clin Chem 1992; 38:2221–3.10.1093/clinchem/38.11.2221Search in Google Scholar
11. De Jonge N, Sewkaransing I, Slinger J, Rijsdijk JJ. Erythrocyte sedimentation rate by the Test-1 analyzer. Clin Chem 2000; 46:881–2.10.1093/clinchem/46.6.881Search in Google Scholar
©2006 by Walter de Gruyter Berlin New York
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