Autoantibody profiling of patients with autoimmune thyroid disease using a new multiplexed immunoassay method
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Renato Tozzoli
Abstract
Background: It has been proposed that only anti-thyroid-peroxidase (TPOAb) and not anti-thyroglobulin (TgAb) antibodies should be assayed in the diagnosis of autoimmune thyroid disease (AITD).
Methods: We analyzed the thyroid autoantibody profile with a multiplex bead array system, which allows simultaneous measurement of thyroid autoantibodies (FIDIS, BioMedical Diagnostics, Marne La Vallée, France). A total of 151 AITD patients (111 Hashimoto's thyroiditis and 40 Graves' disease) and 414 control patients were recruited to the study.
Results: The diagnostic sensitivity of TPOAb was greater than that of TgAb in both Hashimoto's thyroiditis (91.9% vs. 82.5%) and Graves' disease (82.5% and 52.5%, respectively), whereas the diagnostic specificity was similar (92.7% for TPOAb and 92.4% for TgAb).
Conclusions: The presence of isolated positivity for TgAb in 6% of the Hashimoto's thyroiditis patients indicates that the use of only the TPOAb test does not guarantee sufficient diagnostic sensitivity. In the light of recent findings relating to the pathogenetic role of TgAb and TPOAb in Hashimoto's thyroiditis, knowledge of the autoantibody profile could allow the identification of sub-groups of patients with a different degree of activation of the thyroid autoimmune process.
Clin Chem Lab Med 2006;44:837–42.
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©2006 by Walter de Gruyter Berlin New York
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Articles in the same Issue
- Flow-cytometric immunophenotyping of normal and malignant lymphocytes
- Recommendation to treat continuous variable errors like attribute errors
- Polymorphisms associated with apolipoprotein B levels in Greek patients with familial hypercholesterolemia
- Lack of association between α2B-adrenergic receptor polymorphism and risk of restenosis following coronary angioplasty and stent implantation – preliminary report
- Prognostic value of homocysteinemia in patients with congestive heart failure
- Comparability of indices for insulin resistance and insulin secretion determined during oral glucose tolerance tests
- Evaluation of clinical markers of atherosclerosis in young and elderly Japanese adults
- Hypoadiponectinemia is associated with symptomatic atherosclerotic peripheral arterial disease
- Cardiac troponin T and amino-terminal pro-natriuretic peptide concentrations in fetuses in the second trimester and in healthy neonates
- Autoantibody profiling of patients with autoimmune thyroid disease using a new multiplexed immunoassay method
- Fluorimetric determination of activity and isoenzyme composition of N-acetyl-β-D-hexosaminidase in seminal plasma of fertile men and infertile patients with secretory azoospermia
- Increased fructosamine in non-diabetic rheumatoid arthritis patients: role of lipid peroxides and glutathione
- Usefulness of whole blood aggregometry and its comparison with thromboxane generation assay in monitoring acetylsalicylic acid effectiveness – a multiparametric study in rats
- Elevated plasma homocysteine levels in L-dopa-treated Parkinson's disease patients with dyskinesias
- Use of total patient data for indirect estimation of reference intervals for 40 clinical chemical analytes in Turkey
- Use of haemolysis index to estimate potassium concentration in in-vitro haemolysed serum samples
- Longitudinal changes in serum paraoxonase-1 activity throughout normal pregnancy
- Establishment of detailed reference values for luteinizing hormone, follicle stimulating hormone, estradiol, and progesterone during different phases of the menstrual cycle on the Abbott ARCHITECT® analyzer
- Evaluation and quality assessment of glucose concentration measurement in blood by point-of-care testing devices
- Hypothesis on interferences in kinetic interaction of microparticles in solution (KIMS) technology
- Comparability of point-of-care whole-blood electrolyte and substrate testing using a Stat Profile Critical Care Xpress analyzer and standard laboratory methods
- Determination of the length of sedimentation reaction in blood using the TEST 1 system: comparison with the Sedimatic 100 method, turbidimetric fibrinogen levels, and the influence of M-proteins
- Transcriptions and ISO 15189
- Falsely elevated troponin I attributed to inadequate centrifugation using the Access® immunoassay analyzer
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