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No Variation in Hsp70 mRNA Level during Cardiac Surgery in Pediatric Patients Evaluated by Semiquantitative RT-PCR

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Published/Copyright: June 1, 2005
Clinical Chemistry and Laboratory Medicine (CCLM)
From the journal Volume 39 Issue 12

Abstract

We evaluated mRNA expression of the heat shock protein gene, Hsp70-1, by means of a semiquantitative RTPCR in atrial tissue specimens from pediatric patients collected before and after cardiopulmonary bypass surgery for congenital heart diseases, to see whether surgical stress may affect the expression level of this mRNA.

We studied thirty nine pediatric patients (aged 3 months to 15 years) undergoing surgical correction of congenital heart malformation. Twenty-one patients were affected by the tetralogy of Fallot, two by combined atrioventricular septal defects, six by ventricular septal defect, three by atrial septal defect, two by atrioventricular canal defect, two by pulmonary valve stenosis, one by mitral insufficiency, and one by double-outlet right ventricle. Our results showed no significant changes in the Hsp70-1 mRNA expression in atrial tissue of patients before and after cross-clamping (the mean relative expression after cross-clamping was 1.0±0.6 compared to the baseline value). Furthermore, no significant correlations were observed between cross-clamping time and temperature, cardiopulmonary bypass time and mRNA variation. Our study suggests that, during cardioplegia, myocardial tissue does not have an appropriate adaptive response to surgical stress.

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Published Online: 2005-06-01
Published in Print: 2001-12-07

Copyright © 2001 by Walter de Gruyter GmbH & Co. KG

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  1. Contents
  2. Subject Index
  3. Author Index
  4. Summary of the 2nd Sino-European Congress of Laboratory Medicine
  5. Laboratory Diagnosis of Viral Hepatitis in China: The Present and the Future
  6. Molecular Diagnostics in China
  7. Detection of Usual and Atypical Aldehyde Dehydrogenase Alleles by Mismatch Amplification Mutation Assay
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  11. 2nd Sino-European Congress of Laboratory Medicine – Strategies for Laboratory Diagnostics in Medicine
  12. The Problems and Strategy relevant to the Quality Management of Clinical Laboratories
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  15. Determination of Tumor Markers in Serum. Pitfalls and Good Practice
  16. Gas Chromatography-Mass Spectrometry Measurement of 6β-OH-Cortisol/Cortisol Ratio in Human Urine: A Specific Marker of Enzymatic Induction
  17. No Variation in Hsp70 mRNA Level during Cardiac Surgery in Pediatric Patients Evaluated by Semiquantitative RT-PCR
  18. Rapid Detection of Macroprolactin in tHe Form of Prolactin-Immunoglobulin G Complexes by Immunoprecipitation with Anti-human IgG-Agarose
  19. Molecular Characterization of Chromosome 22 Deletions by Short Tandem Repeat Polymorphism (STRP) in Patients with Conotruncal Heart Defects
  20. A Simple and Rapid Analysis of Triplet Repeat Diseases by Expand Long PCR
  21. The Effects of Peroxynitrite on Erythrocytes
  22. The Use of Hirudin as Universal Anticoagulant in Haematology, Clinical Chemistry and Blood Grouping
  23. Comparison of Different Immunoassays for CA 19-9
  24. The Evaluation of Analytical Performance of the Precision G Point-of-Care Glucometer
  25. On Painting and Philosophy: Sir Henry Raeburn
  26. Acknowledgement
  27. Meetings and Awards
https://doi.org/10.1515/CCLM.2001.199

Articles in the same Issue

  1. Contents
  2. Subject Index
  3. Author Index
  4. Summary of the 2nd Sino-European Congress of Laboratory Medicine
  5. Laboratory Diagnosis of Viral Hepatitis in China: The Present and the Future
  6. Molecular Diagnostics in China
  7. Detection of Usual and Atypical Aldehyde Dehydrogenase Alleles by Mismatch Amplification Mutation Assay
  8. Expression of Prostaglandin D Synthase in Ovarian Cancer
  9. Carrier Detection and Prenatal Diagnosis of Hemophilia A
  10. Detection of Oligoclonal Immunoglobulins in the Cerebrospinal Fluid by Immunofixation Electrophoresis
  11. 2nd Sino-European Congress of Laboratory Medicine – Strategies for Laboratory Diagnostics in Medicine
  12. The Problems and Strategy relevant to the Quality Management of Clinical Laboratories
  13. Computer Application to the Laboratory Management in Hospital
  14. The Basis of the Synovial Fluid Analysis
  15. Determination of Tumor Markers in Serum. Pitfalls and Good Practice
  16. Gas Chromatography-Mass Spectrometry Measurement of 6β-OH-Cortisol/Cortisol Ratio in Human Urine: A Specific Marker of Enzymatic Induction
  17. No Variation in Hsp70 mRNA Level during Cardiac Surgery in Pediatric Patients Evaluated by Semiquantitative RT-PCR
  18. Rapid Detection of Macroprolactin in tHe Form of Prolactin-Immunoglobulin G Complexes by Immunoprecipitation with Anti-human IgG-Agarose
  19. Molecular Characterization of Chromosome 22 Deletions by Short Tandem Repeat Polymorphism (STRP) in Patients with Conotruncal Heart Defects
  20. A Simple and Rapid Analysis of Triplet Repeat Diseases by Expand Long PCR
  21. The Effects of Peroxynitrite on Erythrocytes
  22. The Use of Hirudin as Universal Anticoagulant in Haematology, Clinical Chemistry and Blood Grouping
  23. Comparison of Different Immunoassays for CA 19-9
  24. The Evaluation of Analytical Performance of the Precision G Point-of-Care Glucometer
  25. On Painting and Philosophy: Sir Henry Raeburn
  26. Acknowledgement
  27. Meetings and Awards
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