Rapid Detection of Macroprolactin in tHe Form of Prolactin-Immunoglobulin G Complexes by Immunoprecipitation with Anti-human IgG-Agarose
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Johan Schiettecatte
Abstract
Immunoprecipitation with anti-human immunoglobulin G-agarose was evaluated for the detection of prolactin-immunoglobulin G (PRL-IgG) complexes in macroprolactinemic samples from hyperprolactinemic patients (prolactin measured using the automated Elecsys PRL assay; Roche Diagnostics, Mannheim, Germany). Using the polyethylene glycol (PEG) precipitation test on serum samples with PRL above 1000 mIU/l, we detected macroprolactin in 38 out of 175 samples with a recovery below 50%. Gel filtration chromatography on a subset of hyperprolactinemic samples confirmed that macroprolactin was the predominant immunoreactive form of PRL in samples which showed a recovery ≤50%. In 37 out of 38 samples containing macroprolactin, immunoprecipitation with anti-human IgG (anti-hIgG)-agarose revealed a higher PRL-binding (19.1–91.3%) than in the sera containing monomeric PRL (<10%). In conclusion, we found that PEG precipitation detected macroprolactin in 21.7% of samples with elevated PRL levels as measured by the Elecsys PRL assay, and that in the vast majority of these samples the presence of PRL-IgG complexes could be demonstrated by immunoprecipitation with anti-hIgG-agarose. In view of the high prevalence of PRL-IgG complexes in hyperprolactinemic patients and the simplicity of the test, immunoprecipitation with anti-hIgG-agarose might play a role in the routine laboratory handling of hyperprolactinemic samples, especially with regards to PRL assays where PEG causes interference.
Copyright © 2001 by Walter de Gruyter GmbH & Co. KG
Articles in the same Issue
- Contents
- Subject Index
- Author Index
- Summary of the 2nd Sino-European Congress of Laboratory Medicine
- Laboratory Diagnosis of Viral Hepatitis in China: The Present and the Future
- Molecular Diagnostics in China
- Detection of Usual and Atypical Aldehyde Dehydrogenase Alleles by Mismatch Amplification Mutation Assay
- Expression of Prostaglandin D Synthase in Ovarian Cancer
- Carrier Detection and Prenatal Diagnosis of Hemophilia A
- Detection of Oligoclonal Immunoglobulins in the Cerebrospinal Fluid by Immunofixation Electrophoresis
- 2nd Sino-European Congress of Laboratory Medicine – Strategies for Laboratory Diagnostics in Medicine
- The Problems and Strategy relevant to the Quality Management of Clinical Laboratories
- Computer Application to the Laboratory Management in Hospital
- The Basis of the Synovial Fluid Analysis
- Determination of Tumor Markers in Serum. Pitfalls and Good Practice
- Gas Chromatography-Mass Spectrometry Measurement of 6β-OH-Cortisol/Cortisol Ratio in Human Urine: A Specific Marker of Enzymatic Induction
- No Variation in Hsp70 mRNA Level during Cardiac Surgery in Pediatric Patients Evaluated by Semiquantitative RT-PCR
- Rapid Detection of Macroprolactin in tHe Form of Prolactin-Immunoglobulin G Complexes by Immunoprecipitation with Anti-human IgG-Agarose
- Molecular Characterization of Chromosome 22 Deletions by Short Tandem Repeat Polymorphism (STRP) in Patients with Conotruncal Heart Defects
- A Simple and Rapid Analysis of Triplet Repeat Diseases by Expand Long PCR
- The Effects of Peroxynitrite on Erythrocytes
- The Use of Hirudin as Universal Anticoagulant in Haematology, Clinical Chemistry and Blood Grouping
- Comparison of Different Immunoassays for CA 19-9
- The Evaluation of Analytical Performance of the Precision G Point-of-Care Glucometer
- On Painting and Philosophy: Sir Henry Raeburn
- Acknowledgement
- Meetings and Awards
Articles in the same Issue
- Contents
- Subject Index
- Author Index
- Summary of the 2nd Sino-European Congress of Laboratory Medicine
- Laboratory Diagnosis of Viral Hepatitis in China: The Present and the Future
- Molecular Diagnostics in China
- Detection of Usual and Atypical Aldehyde Dehydrogenase Alleles by Mismatch Amplification Mutation Assay
- Expression of Prostaglandin D Synthase in Ovarian Cancer
- Carrier Detection and Prenatal Diagnosis of Hemophilia A
- Detection of Oligoclonal Immunoglobulins in the Cerebrospinal Fluid by Immunofixation Electrophoresis
- 2nd Sino-European Congress of Laboratory Medicine – Strategies for Laboratory Diagnostics in Medicine
- The Problems and Strategy relevant to the Quality Management of Clinical Laboratories
- Computer Application to the Laboratory Management in Hospital
- The Basis of the Synovial Fluid Analysis
- Determination of Tumor Markers in Serum. Pitfalls and Good Practice
- Gas Chromatography-Mass Spectrometry Measurement of 6β-OH-Cortisol/Cortisol Ratio in Human Urine: A Specific Marker of Enzymatic Induction
- No Variation in Hsp70 mRNA Level during Cardiac Surgery in Pediatric Patients Evaluated by Semiquantitative RT-PCR
- Rapid Detection of Macroprolactin in tHe Form of Prolactin-Immunoglobulin G Complexes by Immunoprecipitation with Anti-human IgG-Agarose
- Molecular Characterization of Chromosome 22 Deletions by Short Tandem Repeat Polymorphism (STRP) in Patients with Conotruncal Heart Defects
- A Simple and Rapid Analysis of Triplet Repeat Diseases by Expand Long PCR
- The Effects of Peroxynitrite on Erythrocytes
- The Use of Hirudin as Universal Anticoagulant in Haematology, Clinical Chemistry and Blood Grouping
- Comparison of Different Immunoassays for CA 19-9
- The Evaluation of Analytical Performance of the Precision G Point-of-Care Glucometer
- On Painting and Philosophy: Sir Henry Raeburn
- Acknowledgement
- Meetings and Awards
