Detection of Oligoclonal Immunoglobulins in the Cerebrospinal Fluid by Immunofixation Electrophoresis
-
Xia Shen
Abstract
Many disorders of the central nervous system are associated with increased concentration of cerebrospinal fluid protein due to either an increase in the permeability of blood-brain barrier or the synthesis of immunoglobulins within the central nervous system. Immunofixation electrophoresis was used to detect the immunoglobulins in sera and cerebrospinal fluid in patients with different central nervous system disorders. A strong oligoclonal band, or a single weak monoclonal band, observed in the cerebrospinal fluid but not in the serum, indicates the endogenous immunoglobulin synthesis. In 17 cases of non-infectious central nervous system diseases, we diagnosed one case of progressive multifocal leukoencephalopathy, one case of multiple sclerosis , one case of Guillain-Barre syndrome, and one case of epilepsy. A weak band was observed in both serum and cerebrospinal fluid samples in two cases of cerebral trauma. Out of 25 cases of infectious central nervous system diseases, two cases of nervous system syphilis were positive only in cerebrospinal fluid samples, and 23 cases were negative in both samples. Compared with the standard immunofixation, the application of an enzyme-labeled antibody can significantly increase the sensitivity of the method.
Copyright © 2001 by Walter de Gruyter GmbH & Co. KG
Articles in the same Issue
- Contents
- Subject Index
- Author Index
- Summary of the 2nd Sino-European Congress of Laboratory Medicine
- Laboratory Diagnosis of Viral Hepatitis in China: The Present and the Future
- Molecular Diagnostics in China
- Detection of Usual and Atypical Aldehyde Dehydrogenase Alleles by Mismatch Amplification Mutation Assay
- Expression of Prostaglandin D Synthase in Ovarian Cancer
- Carrier Detection and Prenatal Diagnosis of Hemophilia A
- Detection of Oligoclonal Immunoglobulins in the Cerebrospinal Fluid by Immunofixation Electrophoresis
- 2nd Sino-European Congress of Laboratory Medicine – Strategies for Laboratory Diagnostics in Medicine
- The Problems and Strategy relevant to the Quality Management of Clinical Laboratories
- Computer Application to the Laboratory Management in Hospital
- The Basis of the Synovial Fluid Analysis
- Determination of Tumor Markers in Serum. Pitfalls and Good Practice
- Gas Chromatography-Mass Spectrometry Measurement of 6β-OH-Cortisol/Cortisol Ratio in Human Urine: A Specific Marker of Enzymatic Induction
- No Variation in Hsp70 mRNA Level during Cardiac Surgery in Pediatric Patients Evaluated by Semiquantitative RT-PCR
- Rapid Detection of Macroprolactin in tHe Form of Prolactin-Immunoglobulin G Complexes by Immunoprecipitation with Anti-human IgG-Agarose
- Molecular Characterization of Chromosome 22 Deletions by Short Tandem Repeat Polymorphism (STRP) in Patients with Conotruncal Heart Defects
- A Simple and Rapid Analysis of Triplet Repeat Diseases by Expand Long PCR
- The Effects of Peroxynitrite on Erythrocytes
- The Use of Hirudin as Universal Anticoagulant in Haematology, Clinical Chemistry and Blood Grouping
- Comparison of Different Immunoassays for CA 19-9
- The Evaluation of Analytical Performance of the Precision G Point-of-Care Glucometer
- On Painting and Philosophy: Sir Henry Raeburn
- Acknowledgement
- Meetings and Awards
Articles in the same Issue
- Contents
- Subject Index
- Author Index
- Summary of the 2nd Sino-European Congress of Laboratory Medicine
- Laboratory Diagnosis of Viral Hepatitis in China: The Present and the Future
- Molecular Diagnostics in China
- Detection of Usual and Atypical Aldehyde Dehydrogenase Alleles by Mismatch Amplification Mutation Assay
- Expression of Prostaglandin D Synthase in Ovarian Cancer
- Carrier Detection and Prenatal Diagnosis of Hemophilia A
- Detection of Oligoclonal Immunoglobulins in the Cerebrospinal Fluid by Immunofixation Electrophoresis
- 2nd Sino-European Congress of Laboratory Medicine – Strategies for Laboratory Diagnostics in Medicine
- The Problems and Strategy relevant to the Quality Management of Clinical Laboratories
- Computer Application to the Laboratory Management in Hospital
- The Basis of the Synovial Fluid Analysis
- Determination of Tumor Markers in Serum. Pitfalls and Good Practice
- Gas Chromatography-Mass Spectrometry Measurement of 6β-OH-Cortisol/Cortisol Ratio in Human Urine: A Specific Marker of Enzymatic Induction
- No Variation in Hsp70 mRNA Level during Cardiac Surgery in Pediatric Patients Evaluated by Semiquantitative RT-PCR
- Rapid Detection of Macroprolactin in tHe Form of Prolactin-Immunoglobulin G Complexes by Immunoprecipitation with Anti-human IgG-Agarose
- Molecular Characterization of Chromosome 22 Deletions by Short Tandem Repeat Polymorphism (STRP) in Patients with Conotruncal Heart Defects
- A Simple and Rapid Analysis of Triplet Repeat Diseases by Expand Long PCR
- The Effects of Peroxynitrite on Erythrocytes
- The Use of Hirudin as Universal Anticoagulant in Haematology, Clinical Chemistry and Blood Grouping
- Comparison of Different Immunoassays for CA 19-9
- The Evaluation of Analytical Performance of the Precision G Point-of-Care Glucometer
- On Painting and Philosophy: Sir Henry Raeburn
- Acknowledgement
- Meetings and Awards
