The Effects of Peroxynitrite on Erythrocytes
-
Nurten Türközkan
Abstract
Endotoxin-induced peroxynitrite formation has been demonstrated in plasma. The aim of this study is to evaluate whether this has an effect on erythrocytes. For this purpose erythrocyte 3-nitrotyrosine (3-NT) level, Na+-K+ ATPase and glutathione peroxidase activities were measured both in vivo and in vitro. In vivo peroxynitrite formation was induced in rats by intraperitoneal Escherichia coli (E.coli) injection. Erythrocytes were directly incubated with peroxynitrite in the in vitro experiment. 3-NT levels were measured by reverse-phase HPLC, glutathione peroxidase, and Na+-K+ ATPase activities were measured by spectrophotometric techniques. There was a marked increase in the 3-NT levels in both experiments. However, glutathione peroxidase activity was significantly increased in in vivo experiments, while decreasing in in vitro conditions. Although Na+-K+ ATPase activities were significantly reduced by peroxynitrite in vitro, Na+-K+ ATPase activities were similar in control and E.coli-injected rat erythrocytes. Although nitrating effect of peroxynitrite does not seem to be preventable by endogenous antioxidants, this effect of peroxynitrite may not endanger erythrocytes if the oxidative damage of peroxynitrite is prevented.
Copyright © 2001 by Walter de Gruyter GmbH & Co. KG
Articles in the same Issue
- Contents
- Subject Index
- Author Index
- Summary of the 2nd Sino-European Congress of Laboratory Medicine
- Laboratory Diagnosis of Viral Hepatitis in China: The Present and the Future
- Molecular Diagnostics in China
- Detection of Usual and Atypical Aldehyde Dehydrogenase Alleles by Mismatch Amplification Mutation Assay
- Expression of Prostaglandin D Synthase in Ovarian Cancer
- Carrier Detection and Prenatal Diagnosis of Hemophilia A
- Detection of Oligoclonal Immunoglobulins in the Cerebrospinal Fluid by Immunofixation Electrophoresis
- 2nd Sino-European Congress of Laboratory Medicine – Strategies for Laboratory Diagnostics in Medicine
- The Problems and Strategy relevant to the Quality Management of Clinical Laboratories
- Computer Application to the Laboratory Management in Hospital
- The Basis of the Synovial Fluid Analysis
- Determination of Tumor Markers in Serum. Pitfalls and Good Practice
- Gas Chromatography-Mass Spectrometry Measurement of 6β-OH-Cortisol/Cortisol Ratio in Human Urine: A Specific Marker of Enzymatic Induction
- No Variation in Hsp70 mRNA Level during Cardiac Surgery in Pediatric Patients Evaluated by Semiquantitative RT-PCR
- Rapid Detection of Macroprolactin in tHe Form of Prolactin-Immunoglobulin G Complexes by Immunoprecipitation with Anti-human IgG-Agarose
- Molecular Characterization of Chromosome 22 Deletions by Short Tandem Repeat Polymorphism (STRP) in Patients with Conotruncal Heart Defects
- A Simple and Rapid Analysis of Triplet Repeat Diseases by Expand Long PCR
- The Effects of Peroxynitrite on Erythrocytes
- The Use of Hirudin as Universal Anticoagulant in Haematology, Clinical Chemistry and Blood Grouping
- Comparison of Different Immunoassays for CA 19-9
- The Evaluation of Analytical Performance of the Precision G Point-of-Care Glucometer
- On Painting and Philosophy: Sir Henry Raeburn
- Acknowledgement
- Meetings and Awards
Articles in the same Issue
- Contents
- Subject Index
- Author Index
- Summary of the 2nd Sino-European Congress of Laboratory Medicine
- Laboratory Diagnosis of Viral Hepatitis in China: The Present and the Future
- Molecular Diagnostics in China
- Detection of Usual and Atypical Aldehyde Dehydrogenase Alleles by Mismatch Amplification Mutation Assay
- Expression of Prostaglandin D Synthase in Ovarian Cancer
- Carrier Detection and Prenatal Diagnosis of Hemophilia A
- Detection of Oligoclonal Immunoglobulins in the Cerebrospinal Fluid by Immunofixation Electrophoresis
- 2nd Sino-European Congress of Laboratory Medicine – Strategies for Laboratory Diagnostics in Medicine
- The Problems and Strategy relevant to the Quality Management of Clinical Laboratories
- Computer Application to the Laboratory Management in Hospital
- The Basis of the Synovial Fluid Analysis
- Determination of Tumor Markers in Serum. Pitfalls and Good Practice
- Gas Chromatography-Mass Spectrometry Measurement of 6β-OH-Cortisol/Cortisol Ratio in Human Urine: A Specific Marker of Enzymatic Induction
- No Variation in Hsp70 mRNA Level during Cardiac Surgery in Pediatric Patients Evaluated by Semiquantitative RT-PCR
- Rapid Detection of Macroprolactin in tHe Form of Prolactin-Immunoglobulin G Complexes by Immunoprecipitation with Anti-human IgG-Agarose
- Molecular Characterization of Chromosome 22 Deletions by Short Tandem Repeat Polymorphism (STRP) in Patients with Conotruncal Heart Defects
- A Simple and Rapid Analysis of Triplet Repeat Diseases by Expand Long PCR
- The Effects of Peroxynitrite on Erythrocytes
- The Use of Hirudin as Universal Anticoagulant in Haematology, Clinical Chemistry and Blood Grouping
- Comparison of Different Immunoassays for CA 19-9
- The Evaluation of Analytical Performance of the Precision G Point-of-Care Glucometer
- On Painting and Philosophy: Sir Henry Raeburn
- Acknowledgement
- Meetings and Awards
