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Solution structure of Phl p 3, a major allergen from timothy grass pollen

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Published/Copyright: June 6, 2008
Biological Chemistry
From the journal Volume 389 Issue 7

Abstract

The major 97-aa timothy grass (Phleum pratense) allergen Phl p 3 was recently isolated from an extract of timothy grass pollen. Sequence comparison classifies this protein as a group 3 allergen. The solution structure of Phl p 3 as determined by nuclear magnetic resonance spectroscopy reveals that the protein consists of a core of hydrophobic amino-acid side chains from two β-sheets of five and four anti-parallel β-strands, respectively. This conformation is very similar to the crystal structure published for Phl p 2 and strongly resembles the known conformation of the carboxy-terminal domain of Phl p 1, the major difference being the loop orientations. Phl p 2 and Phl p 3 show virtually identical immunoreactivity, and comparison of the charged surface amino acids of the two proteins gives initial clues as to the IgE recognition epitopes of these proteins.

Keywords: allergen; epitopes; grass pollen; Phl p 3; Phleum pratense; timothy grass
Corresponding author
Received: 2007-11-29
Accepted: 2008-2-27
Published Online: 2008-06-06
Published in Print: 2008-07-01

©2008 by Walter de Gruyter Berlin New York

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  12. Generation of transducible versions of transcription factors Oct4 and Sox2
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  14. Bone marrow-derived mesenchymal stem cell transplantation does not improve quality of muscle reinnervation or recovery of motor function after facial nerve transection in rats
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  17. Genes and Nucleic Acids
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  20. Solution structure of Phl p 3, a major allergen from timothy grass pollen
  21. A hypoallergenic hybrid molecule with increased immunogenicity consisting of derivatives of the major grass pollen allergens, Phl p 2 and Phl p 6
  22. Cell Biology and Signaling
  23. Epigallocatechin gallate inhibits endothelial exocytosis
  24. Proteolysis
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https://doi.org/10.1515/BC.2008.102
Keywords for this article
allergen; epitopes; grass pollen; Phl p 3; Phleum pratense; timothy grass

Articles in the same Issue

  1. Guest Editorial
  2. Highlight: Self-renewal signaling in stem cells
  3. Commentary
  4. Commentary: Highlight on stem cell research
  5. Highlight: Self-Renewal Signaling in Stem Cells
  6. Prospective of Ras signaling in stem cells
  7. Prowling wolves in sheep's clothing: the search for tumor stem cells
  8. Lineage development of hematopoietic stem and progenitor cells
  9. Plasticity after allogeneic hematopoietic stem cell transplantation
  10. Adult progenitor cells in vascular remodeling during atherosclerosis
  11. Oct4 expression revisited: potential pitfalls for data misinterpretation in stem cell research
  12. Generation of transducible versions of transcription factors Oct4 and Sox2
  13. Cytokine combinations differentially influence the SDF-1α-dependent migratory activity of cultivated murine hematopoietic stem and progenitor cells
  14. Bone marrow-derived mesenchymal stem cell transplantation does not improve quality of muscle reinnervation or recovery of motor function after facial nerve transection in rats
  15. In vitro differentiation of reprogrammed murine somatic cells into hepatic precursor cells
  16. The WNT receptor FZD7 contributes to self-renewal signaling of human embryonic stem cells
  17. Genes and Nucleic Acids
  18. Elimination of hop latent viroid upon developmental activation of pollen nucleases
  19. Protein Structure and Function
  20. Solution structure of Phl p 3, a major allergen from timothy grass pollen
  21. A hypoallergenic hybrid molecule with increased immunogenicity consisting of derivatives of the major grass pollen allergens, Phl p 2 and Phl p 6
  22. Cell Biology and Signaling
  23. Epigallocatechin gallate inhibits endothelial exocytosis
  24. Proteolysis
  25. Dimerization of endogenous MT1-MMP is a regulatory step in the activation of the 72-kDa gelatinase MMP-2 on fibroblasts and fibrosarcoma cells
  26. Reconstitution of human azurocidin catalytic triad and proteolytic activity by site-directed mutagenesis
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