Cytokine combinations differentially influence the SDF-1α-dependent migratory activity of cultivated murine hematopoietic stem and progenitor cells
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Susannah H. Kassmer
Abstract
Stromal cell-derived factor-1α (SDF-1α) is a strong migratory stimulant for hematopoietic stem and progenitor cells (HSPCs). The hematopoietic cytokines thrombopoietin (TPO), Flt3-ligand (FL), stem cell factor (SCF) and interleukin 11 (IL-11) are able to stimulate amplification of primitive murine hematopoietic stem cells (HSCs) in vitro. The effects of these cytokines on SDF-1α-induced migratory activity of murine Lin-c-kit+ HSPC were analyzed by cultivation of these cells in the presence of 12 combinations of FL, TPO, SCF and IL-11. Migratory activity was measured in a three-dimensional collagen matrix using time-lapse video microscopy. Each cytokine combination had a distinct effect on SDF-1α-stimulated migratory activity. For instance, FL- and SCF-cultivated cells showed a high migratory SDF-1α response, while cells cultivated with SCF, TPO and IL-11 did not react to SDF-1α stimulation with an elevated migration rate. Our data indicate that the differences in the migratory SDF-1α response are not related to different CXCR4 expression levels, but rather to the differential engagement of the CXCR4-dependent MAPKp42/44 and PI3K signal transduction pathways. This indicates that hematopoietic cytokines can have a significant impact on SDF-1α-stimulated migratory activity and the underlying intracellular signaling processes in cultivated HSPCs.
©2008 by Walter de Gruyter Berlin New York
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Articles in the same Issue
- Guest Editorial
- Highlight: Self-renewal signaling in stem cells
- Commentary
- Commentary: Highlight on stem cell research
- Highlight: Self-Renewal Signaling in Stem Cells
- Prospective of Ras signaling in stem cells
- Prowling wolves in sheep's clothing: the search for tumor stem cells
- Lineage development of hematopoietic stem and progenitor cells
- Plasticity after allogeneic hematopoietic stem cell transplantation
- Adult progenitor cells in vascular remodeling during atherosclerosis
- Oct4 expression revisited: potential pitfalls for data misinterpretation in stem cell research
- Generation of transducible versions of transcription factors Oct4 and Sox2
- Cytokine combinations differentially influence the SDF-1α-dependent migratory activity of cultivated murine hematopoietic stem and progenitor cells
- Bone marrow-derived mesenchymal stem cell transplantation does not improve quality of muscle reinnervation or recovery of motor function after facial nerve transection in rats
- In vitro differentiation of reprogrammed murine somatic cells into hepatic precursor cells
- The WNT receptor FZD7 contributes to self-renewal signaling of human embryonic stem cells
- Genes and Nucleic Acids
- Elimination of hop latent viroid upon developmental activation of pollen nucleases
- Protein Structure and Function
- Solution structure of Phl p 3, a major allergen from timothy grass pollen
- A hypoallergenic hybrid molecule with increased immunogenicity consisting of derivatives of the major grass pollen allergens, Phl p 2 and Phl p 6
- Cell Biology and Signaling
- Epigallocatechin gallate inhibits endothelial exocytosis
- Proteolysis
- Dimerization of endogenous MT1-MMP is a regulatory step in the activation of the 72-kDa gelatinase MMP-2 on fibroblasts and fibrosarcoma cells
- Reconstitution of human azurocidin catalytic triad and proteolytic activity by site-directed mutagenesis
