Recombinant kallikrein expression: site-specific integration for hK6 production in human cells
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Nathalie Heuzé-Vourc'h
Abstract
Kallikreins have been implicated in carcinogenesis and are promising biomarkers for the diagnosis and follow-up of various cancers. To evaluate the functions and clinical interest of kallikreins, it is important to be able to produce them as recombinant proteins. Here we summarize the various strategies used to produce kallikreins, emphasizing their advantages and limitations. We also describe an approach to achieve high-level production of kallikreins, such as hK6, with correct folding and activity, combining an expression system with targeted transgene integration and an efficient cultivation device to increase yield, the CELLine bioreactor. This novel method for recombinant kallikrein production will be useful to study their bio-pathological functions and to develop anti-bodies.
References
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Artikel in diesem Heft
- The First International Symposium on Kallikreins
- A comprehensive nomenclature for serine proteases with homology to tissue kallikreins
- The kallikrein world: an update on the human tissue kallikreins
- Cellular distribution of human tissue kallikreins: immunohistochemical localization
- The tissue kallikrein-kinin system protects against cardiovascular and renal diseases and ischemic stroke independently of blood pressure reduction
- Proteinase-mediated cell signalling: targeting proteinase-activated receptors (PARs) by kallikreins and more
- Recombinant kallikrein expression: site-specific integration for hK6 production in human cells
- Kallikrein-related peptidase (KLK) family mRNA variants and protein isoforms in hormone-related cancers: do they have a function?
- The role of kallikrein-related peptidases in prostate cancer: potential involvement in an epithelial to mesenchymal transition
- Human kallikrein 10, a predictive marker for breast cancer
- Activation and enzymatic characterization of recombinant human kallikrein 8
- Human tissue kallikrein 9: production of recombinant proteins and specific antibodies
- The human kallikrein 10 promoter contains a functional retinoid response element
- Human kallikrein 4: enzymatic activity, inhibition, and degradation of extracellular matrix proteins
- Kallikrein-related peptidase 14 may be a major contributor to trypsin-like proteolytic activity in human stratum corneum
- A sensitive proximity ligation assay for active PSA
- Multiple mechanisms underlie the aberrant expression of the human kallikrein 6 gene in breast cancer
- Expression of the human kallikrein genes 10 (KLK10) and 11 (KLK11) in cancerous and non-cancerous lung tissues
- mRNA expression analysis of human kallikrein 11 (KLK11) may be useful in the discrimination of benign prostatic hyperplasia from prostate cancer after needle prostate biopsy
- The epigenetic basis for the aberrant expression of kallikreins in human cancers
- Improved prostate cancer detection with a human kallikrein 11 and percentage free PSA-based artificial neural network
- Overexpression of the human tissue kallikrein genes KLK4, 5, 6, and 7 increases the malignant phenotype of ovarian cancer cells
- Inhibition profiles of human tissue kallikreins by serine protease inhibitors
- Kallikrein-mediated cell signalling: targeting proteinase-activated receptors (PARs)
Artikel in diesem Heft
- The First International Symposium on Kallikreins
- A comprehensive nomenclature for serine proteases with homology to tissue kallikreins
- The kallikrein world: an update on the human tissue kallikreins
- Cellular distribution of human tissue kallikreins: immunohistochemical localization
- The tissue kallikrein-kinin system protects against cardiovascular and renal diseases and ischemic stroke independently of blood pressure reduction
- Proteinase-mediated cell signalling: targeting proteinase-activated receptors (PARs) by kallikreins and more
- Recombinant kallikrein expression: site-specific integration for hK6 production in human cells
- Kallikrein-related peptidase (KLK) family mRNA variants and protein isoforms in hormone-related cancers: do they have a function?
- The role of kallikrein-related peptidases in prostate cancer: potential involvement in an epithelial to mesenchymal transition
- Human kallikrein 10, a predictive marker for breast cancer
- Activation and enzymatic characterization of recombinant human kallikrein 8
- Human tissue kallikrein 9: production of recombinant proteins and specific antibodies
- The human kallikrein 10 promoter contains a functional retinoid response element
- Human kallikrein 4: enzymatic activity, inhibition, and degradation of extracellular matrix proteins
- Kallikrein-related peptidase 14 may be a major contributor to trypsin-like proteolytic activity in human stratum corneum
- A sensitive proximity ligation assay for active PSA
- Multiple mechanisms underlie the aberrant expression of the human kallikrein 6 gene in breast cancer
- Expression of the human kallikrein genes 10 (KLK10) and 11 (KLK11) in cancerous and non-cancerous lung tissues
- mRNA expression analysis of human kallikrein 11 (KLK11) may be useful in the discrimination of benign prostatic hyperplasia from prostate cancer after needle prostate biopsy
- The epigenetic basis for the aberrant expression of kallikreins in human cancers
- Improved prostate cancer detection with a human kallikrein 11 and percentage free PSA-based artificial neural network
- Overexpression of the human tissue kallikrein genes KLK4, 5, 6, and 7 increases the malignant phenotype of ovarian cancer cells
- Inhibition profiles of human tissue kallikreins by serine protease inhibitors
- Kallikrein-mediated cell signalling: targeting proteinase-activated receptors (PARs)