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Expression of the human kallikrein genes 10 (KLK10) and 11 (KLK11) in cancerous and non-cancerous lung tissues

  • Chris Planque , Mireille Aïnciburu , Nathalie Heuzé-Vourc'h , Sandra Régina , Michèle de Monte and Yves Courty
Published/Copyright: June 26, 2006
Biological Chemistry
From the journal Volume 387 Issue 6

Abstract

Only one transcript for KLK10 was identified by RT-PCR in lung tissue, whereas KLK11 expressed at least four alternative transcripts. Quantitative analysis of KLK10 and KLK11 expression levels was assessed by real-time PCR, in a cohort of 47 patients with non-small-cell lung cancer (NSCLC). Expression levels of these genes were widely distributed in the population studied. Multivariate analysis revealed a correlation between KLK10 over-expression and the squamous cell carcinoma histotype (p=0.034). There was no correlation between gene expression and patient survival. Overall, both genes behaved similarly (p<0.001). These results suggest a co-regulation of KLK10 and KLK11 expression in lung and a lack of KLK10 suppressor role in NSCLC. Finally, our findings indicate that these genes are likely involved in normal physiology processes in bronchus.

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References

Bhattacharjee, A., Richards, W.G., Staunton, J., Li, C., Monti, S., Vasa, P., Ladd, C., Beheshti, J., Bueno, R., Gillette, M., et al. (2001). Classification of human lung carcinomas by mRNA expression profiling reveals distinct adenocarcinoma subclasses. Proc. Natl. Acad. Sci. USA98, 13790–13795.10.1073/pnas.191502998Search in Google Scholar

Borgono, C.A. and Diamandis, E.P. (2004). The emerging roles of human tissue kallikreins in cancer. Nat. Rev. Cancer4, 876–890.10.1038/nrc1474Search in Google Scholar

Borgono, C.A., Fracchioli, S., Yousef, G.M., Rigault de la Longrais, I.A., Luo, L.Y., Soosaipillai, A., Puopolo, M., Grass, L., Scorilas, A., Diamandis, E.P., and Katsaros, D. (2003). Favorable prognostic value of tissue human kallikrein 11 (hK11) in patients with ovarian carcinoma. Int. J. Cancer106, 605–610.10.1002/ijc.11296Search in Google Scholar

Borgono, C.A., Michael, I.P., and Diamandis, E.P. (2004). Human tissue kallikreins: physiologic roles and applications in cancer. Mol. Cancer Res.2, 257–280.10.1158/1541-7786.257.2.5Search in Google Scholar

Clements, J.A., Willemsen, N.M., Myers, S.A., and Dong, Y. (2004). The tissue kallikrein family of serine proteases: functional roles in human disease and potential as clinical biomarkers. Crit. Rev. Clin. Lab. Sci.41, 265–312.10.1080/10408360490471931Search in Google Scholar

de Monte, M., Nonnenmacher, H., Brignon, N., Ullmann, M., and Martin, J.P. (2002). A multivariate statistical analysis to follow the course of disease after infection of cats with different strains of the feline immunodeficiency virus (FIV). J. Virol. Methods103, 157–170.10.1016/S0166-0934(02)00024-1Search in Google Scholar

Diamandis, E.P., Okui, A., Mitsui, S., Luo, L.Y., Soosaipillai, A., Grass, L., Nakamura, T., Howarth, D.J., and Yamaguchi, N. (2002). Human kallikrein 11: a new biomarker of prostate and ovarian carcinoma. Cancer Res.62, 295–300.Search in Google Scholar

Diamandis, E.P., Borgono, C.A., Scorilas, A., Harbeck, N., Dorn, J., and Schmitt, M. (2004). Human kallikrein 11: an indicator of favorable prognosis in ovarian cancer patients. Clin. Biochem.37, 823–829.10.1016/j.clinbiochem.2004.04.009Search in Google Scholar PubMed

Garber, M.E., Troyanskaya, O.G., Schluens, K., Petersen, S., Thaesler, Z., Pacyna-Gengelbach, M., van de Rijn, M., Rosen, G.D., Perou, C.M., Whyte, R.I., et al. (2001). Diversity of gene expression in adenocarcinoma of the lung. Proc. Natl. Acad. Sci. USA98, 13784–13789.10.1073/pnas.241500798Search in Google Scholar PubMed PubMed Central

Goyal, J., Smith, K.M., Cowan, J.M., Wazer, D.E., Lee, S.W., and Band, V. (1998). The role for NES1 serine protease as a novel tumor suppressor. Cancer Res.58, 4782–4786.Search in Google Scholar

Houtmeyers, E., Gosselink, R., Gayan-Ramirez, G., and Decramer, M. (1999). Regulation of mucociliary clearance in health and disease. Eur. Respir. J.13, 1177–1188.10.1034/j.1399-3003.1999.13e39.xSearch in Google Scholar PubMed

Kurlender, L., Borgono, C., Michael, I.P., Obiezu, C., Elliott, M.B., Yousef, G.M., and Diamandis, E.P. (2005). A survey of alternative transcripts of human tissue kallikrein genes. Biochim. Biophys. Acta1755, 1–14.10.1016/j.bbcan.2005.02.001Search in Google Scholar PubMed

Liu, X.L., Wazer, D.E., Watanabe, K., and Band, V. (1996). Identification of a novel serine protease-like gene, the expression of which is down-regulated during breast cancer progression. Cancer Res.56, 3371–3379.Search in Google Scholar

Luo, L.Y., Bunting, P., Scorilas, A., and Diamandis, E.P. (2001a). Human kallikrein 10: a novel tumor marker for ovarian carcinoma? Clin. Chim. Acta306, 111–118.10.1016/S0009-8981(01)00401-6Search in Google Scholar

Luo, L.Y., Grass, L., Howarth, D.J., Thibault, P., Ong, H., and Diamandis, E.P. (2001b). Immunofluorometric assay of human kallikrein 10 and its identification in biological fluids and tissues. Clin. Chem.47, 237–246.10.1093/clinchem/47.2.237Search in Google Scholar

Luo, L.Y., Rajpert-De Meyts, E.R., Jung, K., and Diamandis, E.P. (2001c). Expression of the normal epithelial cell-specific 1 (NES1; KLK10) candidate tumour suppressor gene in normal and malignant testicular tissue. Br. J. Cancer85, 220–224.10.1054/bjoc.2001.1870Search in Google Scholar

Mitsui, S., Yamada, T., Okui, A., Kominami, K., Uemura, H., and Yamaguchi, N. (2000). A novel isoform of a kallikrein-like protease, TLSP/hippostasin, (PRSS20), is expressed in the human brain and prostate. Biochem. Biophys. Res. Commun.272, 205–211.10.1006/bbrc.2000.2761Search in Google Scholar

Nakamura, T., Mitsui, S., Okui, A., Kominami, K., Nomoto, T., Ukimura, O., Kawauchi, A., Miki, T., and Yamaguchi, N. (2001). Alternative splicing isoforms of hippostasin (PRSS20/KLK11) in prostate cancer cell lines. Prostate49, 72–78.10.1002/pros.1119Search in Google Scholar

Nakamura, T., Mitsui, S., Okui, A., Miki, T., and Yamaguchi, N. (2003a). Molecular cloning and expression of a variant form of hippostasin/KLK11 in prostate. Prostate54, 299–305.10.1002/pros.10191Search in Google Scholar

Nakamura, T., Stephan, C., Scorilas, A., Yousef, G.M., Jung, K,. and Diamandis, E.P. (2003b). Quantitative analysis of hippostasin/KLK11 gene expression in cancerous and noncancerous prostatic tissues. Urology61, 1042–1046.10.1016/S0090-4295(02)02443-3Search in Google Scholar

Obiezu, C.V. and Diamandis, E.P. (2005). Human tissue kallikrein gene family: applications in cancer. Cancer Lett.224, 1–22.10.1016/j.canlet.2004.09.024Search in Google Scholar PubMed

Pampalakis, G., Kurlender, L., Diamandis, E.P., and Sotiropoulou, G. (2004). Cloning and characterization of novel isoforms of the human kallikrein 6 gene. Biochem. Biophys. Res. Commun.320, 54–61.10.1016/j.bbrc.2004.04.205Search in Google Scholar PubMed

Petraki, C.D., Karavana, V.N., Luo, L.Y., and Diamandis, E.P. (2002). Human kallikrein 10 expression in normal tissues by immunohistochemistry. J. Histochem. Cytochem.50, 1247–1261.10.1177/002215540205000912Search in Google Scholar PubMed

Petraki, C.D., Gregorakis, A.K., Papanastasiou, P.A., Karavana, V.N., Luo, L.Y., and Diamandis, E.P. (2003). Immunohistochemical localization of human kallikreins 6, 10 and 13 in benign and malignant prostatic tissues. Prostate Cancer Prostatic Dis.6, 223–227.10.1038/sj.pcan.4500674Search in Google Scholar

Planque, C., de Monte, M., Guyetant, S., Rollin, J., Desmazes, C., Panel, V., Lemarie, E., and Courty, Y. (2005). KLK5 and KLK7, two members of the human tissue kallikrein family, are differentially expressed in lung cancer. Biochem. Biophys. Res. Commun.329, 1260–1266.10.1016/j.bbrc.2005.02.100Search in Google Scholar

Ramakers, C., Ruijter, J.M., Deprez, R.H., and Moorman, A.F. (2003). Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data. Neurosci. Lett.339, 62–66.10.1016/S0304-3940(02)01423-4Search in Google Scholar

Shvartsman, H.S., Lu, K.H., Lee, J., Lillie, J., Deavers, M.T., Clifford, S., Wolf, J.K., Mills, G.B., Bast, R.C. Jr., Gershenson, D.M., and Schmandt, R. (2003). Overexpression of kallikrein 10 in epithelial ovarian carcinomas. Gynecol. Oncol.90, 44–50.10.1016/S0090-8258(03)00257-9Search in Google Scholar

Yoshida, S., Taniguchi, M., Suemoto, T., Oka, T., He, X., and Shiosaka, S. (1998). cDNA cloning and expression of a novel serine protease, TLSP. Biochim. Biophys. Acta1399, 225–228.10.1016/S0167-4781(98)00116-XSearch in Google Scholar

Yousef, G.M., Borgono, C.A., White, N.M., Robb, J.D., Michael, I.P., Oikonomopoulou, K., Khan, S., and Diamandis, E.P. (2004a). In silico analysis of the human kallikrein gene 6. Tumour Biol.25, 282–289.10.1159/000081393Search in Google Scholar PubMed

Yousef, G.M., Yacoub, G.M., Polymeris, M.E., Popalis, C., Soosaipillai, A., and Diamandis, E.P. (2004b). Kallikrein gene downregulation in breast cancer. Br. J. Cancer90, 167–172.10.1038/sj.bjc.6601451Search in Google Scholar PubMed PubMed Central

Zarghami, N., Levesque, M., D'Costa, M., Angelopoulou, K., and Diamandis, E.P. (1997). Frequency of expression of prostate-specific antigen mRNA in lung tumors. Am. J. Clin. Pathol.108, 184–190.10.1093/ajcp/108.2.184Search in Google Scholar PubMed

Published Online: 2006-06-26
Published in Print: 2006-06-01

©2006 by Walter de Gruyter Berlin New York

Articles in the same Issue

  1. The First International Symposium on Kallikreins
  2. A comprehensive nomenclature for serine proteases with homology to tissue kallikreins
  3. The kallikrein world: an update on the human tissue kallikreins
  4. Cellular distribution of human tissue kallikreins: immunohistochemical localization
  5. The tissue kallikrein-kinin system protects against cardiovascular and renal diseases and ischemic stroke independently of blood pressure reduction
  6. Proteinase-mediated cell signalling: targeting proteinase-activated receptors (PARs) by kallikreins and more
  7. Recombinant kallikrein expression: site-specific integration for hK6 production in human cells
  8. Kallikrein-related peptidase (KLK) family mRNA variants and protein isoforms in hormone-related cancers: do they have a function?
  9. The role of kallikrein-related peptidases in prostate cancer: potential involvement in an epithelial to mesenchymal transition
  10. Human kallikrein 10, a predictive marker for breast cancer
  11. Activation and enzymatic characterization of recombinant human kallikrein 8
  12. Human tissue kallikrein 9: production of recombinant proteins and specific antibodies
  13. The human kallikrein 10 promoter contains a functional retinoid response element
  14. Human kallikrein 4: enzymatic activity, inhibition, and degradation of extracellular matrix proteins
  15. Kallikrein-related peptidase 14 may be a major contributor to trypsin-like proteolytic activity in human stratum corneum
  16. A sensitive proximity ligation assay for active PSA
  17. Multiple mechanisms underlie the aberrant expression of the human kallikrein 6 gene in breast cancer
  18. Expression of the human kallikrein genes 10 (KLK10) and 11 (KLK11) in cancerous and non-cancerous lung tissues
  19. mRNA expression analysis of human kallikrein 11 (KLK11) may be useful in the discrimination of benign prostatic hyperplasia from prostate cancer after needle prostate biopsy
  20. The epigenetic basis for the aberrant expression of kallikreins in human cancers
  21. Improved prostate cancer detection with a human kallikrein 11 and percentage free PSA-based artificial neural network
  22. Overexpression of the human tissue kallikrein genes KLK4, 5, 6, and 7 increases the malignant phenotype of ovarian cancer cells
  23. Inhibition profiles of human tissue kallikreins by serine protease inhibitors
  24. Kallikrein-mediated cell signalling: targeting proteinase-activated receptors (PARs)
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