Jasmonate-Induced Lipid Peroxidation in Barley Leaves Initiated by Distinct 13-LOX Forms of Chloroplasts
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A. Bachmann
Abstract
In addition to a previously characterized 13-lipoxygenase of 100 kDa encoded by LOX2:Hv:1 [Vörös et al., Eur. J. Biochem. 251 (1998), 36 44], two fulllength cDNAs (LOX2:Hv:2, LOX2:Hv:3) were isolated from barley leaves (Hordeum vulgare cv. Salome) and characterized. Both of them encode 13-lipoxygenases with putative target sequences for chloroplast import. Immunogold labeling revealed preferential, if not exclusive, localization of lipoxygenase proteins in the stroma. The ultrastructure of the chloroplast was dramatically altered following methyl jasmonate treatment, indicated by a loss of thylakoid membranes, decreased number of stacks and appearance of numerous osmiophilic globuli. The three 13-lipoxygenases are differentially expressed during treatment with jasmonate, salicylate, glucose or sorbitol. Metabolite profiling of free linolenic acid and free linoleic acid, the substrates of lipoxygenases, in water floated or jasmonatetreated leaves revealed preferential accumulation of linolenic acid. Remarkable amounts of free 9- as well as 13-hydroperoxy linolenic acid were found. In addition, metabolites of these hydroperoxides, such as the hydroxy derivatives and the respective aldehydes, appeared following methyl jasmonate treatment. These findings were substantiated by metabolite profiling of isolated chloroplasts, and subfractions including the envelope, the stroma and the thylakoids, indicating a preferential occurrence of lipoxygenasederived products in the stroma and in the envelope. These data revealed jasmonateinduced activation of the hydroperoxide lyase and reductase branch within the lipoxygenase pathway and suggest differential activity of the three 13-lipoxygenases under different stress conditions.
Copyright © 2002 by Walter de Gruyter GmbH & Co. KG
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Artikel in diesem Heft
- Biological Chemistry 125th Anniversary
- Kai Simons Felix Hoppe-Seyler Lecturer 2002
- Lipid Rafts in Protein Sorting and Cell Polarity in Budding Yeast Saccharomyces cerevisiae
- Paper of the Year 2001
- RNA-Mediated RNA Degradation in Transgene- and Virus-Induced Gene Silencing
- Immunopharmacology of CpG DNA
- Induction and Regulation of Endogenous Granulocyte Colony-Stimulating Factor Formation
- In the Cellular Garden of Forking Paths: How p38 MAPKs Signal for Downstream Assistance
- The Human Steroid Hydroxylases CYP11B1 and CYP11B2
- Rate-Limiting Steps in Cytochrome P450 Catalysis
- YidC, a Newly Defined Evolutionarily Conserved Protein, Mediates Membrane Protein Assembly in Bacteria
- Vascular Endothelial Growth Factor Receptor Family Genes: When Did the Three Genes Phylogenetically Segregate?
- The TNFTNF Receptor System
- Fast Regulation of Cytochrome P450 Activities by Phosphorylation and Consequences for Drug Metabolism and Toxicity
- Protein Transduction: A Novel Tool for Tissue Regeneration
- NF-κB A Potential Therapeutic Target for Inhibition of Human Cytomegalovirus (Re)activation?
- Three-State Equilibrium of Escherichia coli Trigger Factor
- Binding of a C-Terminal Fragment (Residues 369 to 435) of Vitamin D-Binding Protein to Actin
- The Hyaluronan-Binding Serine Protease from Human Plasma Cleaves HMW and LMW Kininogen and Releases Bradykinin
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- Real-Time Determination of Telomerase Activity in Cell Extracts Using an Optical Biosensor
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