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Muscle Phosphorylase Kinase Is Not a Substrate of AMP-Activated Protein Kinase

  • A. Beyer , A. Kitzerow , B. Crute , B.E. Kemp , L.A. Witters and L.M.G. Heilmeyer jr
Published/Copyright: July 5, 2005
Biological Chemistry
From the journal Volume 381 Issue 5-6

Abstract

AMP-activated protein kinase (AMPK) and cAMP-dependent protein kinase (cAMPK) have been reported to phosphorylate sites on phosphorylase kinase (PhK). Their target residues Ser 1018 and Ser 1020, respectively, are located in the so-called multi-phosphorylation domain in the PhK α subunit. In PhK preparations, only one of these serines is phosphorylated, but never both of them. The aim of this study was to determine whether phosphorylation by cAMPK or AMPK would influence subsequent phosphorylation by the other kinase. Surprisingly, employing four different PhK substrates, it could be demonstrated that, in contradiction to previous reports, PhK is not phosphorylated by AMPK.

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Published Online: 2005-07-05
Published in Print: 2000-06-21

Copyright © 2000 by Walter de Gruyter GmbH & Co. KG

Articles in the same Issue

  1. Highlight: GTP Binding Proteins Central Regulators in Cell Biology
  2. Signal Transduction and Post-Transcriptional Gene Expression
  3. Regulation of GTPases in the Bacterial Translation Machinery
  4. GTPase Mechanisms and Functions of Translation Factors on the Ribosome
  5. The Role of Heterotrimeric G Proteins in Platelet Activation
  6. Upstream and Downstream of Ran GTPase
  7. Nogo-A, a Potent Inhibitor of Neurite Outgrowth and Regeneration
  8. Rho GTPases as Targets of Bacterial Protein Toxins
  9. A Conserved Gβ Binding (GBB) Sequence Motif in Ste20p/PAK Family Protein Kinases
  10. Identification of a CpG Island in the Human LRP-2 Gene and Analysis of Its Methylation Status in Parathyroid Adenomas
  11. Theoretical Description of the Direct Exponential Amplification and Sequencing (DEXAS) Method
  12. Adenine Nucleotide N-Glycosidase Activity of the A-Chain of Cinnamomin Characterized by 1H-Nuclear Magnetic Resonance
  13. Msb4p, a Protein Involved in Cdc42p-Dependent Organization of the Actin Cytoskeleton, Is a Ypt/Rab-Specific GAP
  14. Muscle Phosphorylase Kinase Is Not a Substrate of AMP-Activated Protein Kinase
  15. Cationic Lipopolyamines Induce Degradation of PrPSc in Scrapie-Infected Mouse Neuroblastoma Cells
  16. Glycosylphosphatidylinositol-Specific Phospholipase D of Human Serum Activity Modulation by Naturally Occurring Amphiphiles
  17. Retrieval of the mrp2 Gene Encoded Conjugate Export Pump from the Canalicular Membrane Contributes to Cholestasis Induced by tert-Butyl Hydroperoxide and Chloro-Dinitrobenzene
  18. Matrix Metalloproteinases-2, -3, -7, -9 and -10, But Not MMP-11, Are Differentially Expressed in Normal, Benign Tumorigenic and Malignant Human Keratinocyte Cell Lines
  19. Human Keratinocyte Cell Lines Differ in the Expression of the Collagenolytic Matrix Metallo-proteinases-1, -8, and -13 and of TIMP-1
  20. Inducibility of the Streptomyces traRts107-Ptra Expression Cassette in Mycobacterium smegmatis
  21. Shortest Known Prion Protein Allele in Highly BSE-Susceptible Lemurs
  22. Prostaglandin Deficiency Promotes Sensitization of Adenylyl Cyclase
https://doi.org/10.1515/BC.2000.060

Articles in the same Issue

  1. Highlight: GTP Binding Proteins Central Regulators in Cell Biology
  2. Signal Transduction and Post-Transcriptional Gene Expression
  3. Regulation of GTPases in the Bacterial Translation Machinery
  4. GTPase Mechanisms and Functions of Translation Factors on the Ribosome
  5. The Role of Heterotrimeric G Proteins in Platelet Activation
  6. Upstream and Downstream of Ran GTPase
  7. Nogo-A, a Potent Inhibitor of Neurite Outgrowth and Regeneration
  8. Rho GTPases as Targets of Bacterial Protein Toxins
  9. A Conserved Gβ Binding (GBB) Sequence Motif in Ste20p/PAK Family Protein Kinases
  10. Identification of a CpG Island in the Human LRP-2 Gene and Analysis of Its Methylation Status in Parathyroid Adenomas
  11. Theoretical Description of the Direct Exponential Amplification and Sequencing (DEXAS) Method
  12. Adenine Nucleotide N-Glycosidase Activity of the A-Chain of Cinnamomin Characterized by 1H-Nuclear Magnetic Resonance
  13. Msb4p, a Protein Involved in Cdc42p-Dependent Organization of the Actin Cytoskeleton, Is a Ypt/Rab-Specific GAP
  14. Muscle Phosphorylase Kinase Is Not a Substrate of AMP-Activated Protein Kinase
  15. Cationic Lipopolyamines Induce Degradation of PrPSc in Scrapie-Infected Mouse Neuroblastoma Cells
  16. Glycosylphosphatidylinositol-Specific Phospholipase D of Human Serum Activity Modulation by Naturally Occurring Amphiphiles
  17. Retrieval of the mrp2 Gene Encoded Conjugate Export Pump from the Canalicular Membrane Contributes to Cholestasis Induced by tert-Butyl Hydroperoxide and Chloro-Dinitrobenzene
  18. Matrix Metalloproteinases-2, -3, -7, -9 and -10, But Not MMP-11, Are Differentially Expressed in Normal, Benign Tumorigenic and Malignant Human Keratinocyte Cell Lines
  19. Human Keratinocyte Cell Lines Differ in the Expression of the Collagenolytic Matrix Metallo-proteinases-1, -8, and -13 and of TIMP-1
  20. Inducibility of the Streptomyces traRts107-Ptra Expression Cassette in Mycobacterium smegmatis
  21. Shortest Known Prion Protein Allele in Highly BSE-Susceptible Lemurs
  22. Prostaglandin Deficiency Promotes Sensitization of Adenylyl Cyclase
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